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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
17/11/2021 |
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Data da última atualização: |
17/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BEZERRA, Y. C. dos A.; MARQUES, J. P. R.; STIPP, L. C. L.; ATTÍLIO, L. B.; ASTUA, J. de F.; MOURÃO FILHO, F. de A. A. |
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Afiliação: |
YANE CAROLINE DOS ANJOS BEZERRA, USP; JOÃO PAULO RODRIGUES MARQUES, USP; LILIANE CRISTINA LIBORIO STIPP, USP; LÍSIA BORGES ATTÍLIO, USP; JULIANA DE FREITAS ASTUA, CNPMF; FRANCISCO DE ASSIS ALVES MOURÃO FILHO, USP. |
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Título: |
How to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Revista Brasileira de Fruticultura, Jaboticabal, v. 43, n. 4,e-005, 2021. |
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ISSN: |
1806-9967 |
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Idioma: |
Português |
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Conteúdo: |
New approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ?Carrizo? citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (?-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ?Rangpur? lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissuespecific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance. |
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Thesagro: |
Fruta Cítrica. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227820/1/How-to-drive-phloem-gene-expression.pdf
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Marc: |
LEADER 02150naa a2200205 a 4500
001 2136203
005 2021-11-17
008 2021 bl uuuu u00u1 u #d
022 $a1806-9967
100 1 $aBEZERRA, Y. C. dos A.
245 $aHow to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters.$h[electronic resource]
260 $c2021
520 $aNew approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ?Carrizo? citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (?-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ?Rangpur? lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissuespecific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance.
650 $aFruta Cítrica
700 1 $aMARQUES, J. P. R.
700 1 $aSTIPP, L. C. L.
700 1 $aATTÍLIO, L. B.
700 1 $aASTUA, J. de F.
700 1 $aMOURÃO FILHO, F. de A. A.
773 $tRevista Brasileira de Fruticultura, Jaboticabal$gv. 43, n. 4,e-005, 2021.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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Tipo/Formato |
Classificação |
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Registro |
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| Registros recuperados : 179 | |
| 101. |  | GARITA, L. C.; TASSI, A. D.; CALEGARIO, R. F.; ASTUA, J. de F.; SALAROLI, R. B.; ROMÃO, G. O.; KITAJIMA, E. W. Experimental host range of Citrus leprosis virus C (CiLV-C). Tropical Plant Pathology, v. 39, n.1, p.043-055, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 102. | | PEREIRA, F. M. V.; MILORI, D. M. B. P.; VENANCIO, A. L.; RUSSO, M. de S. T.; MARTINS, P. K.; ASTUA, J. de F. Evaluation of the effects of Candidatus Liberibacter asiaticus on inoculated citrus plants using laser-induced breakdown spectroscopy (LIBS) and chemometrics tools. Talanta, Oxford, v. 83, n. 2, p. 351-356, dez. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 103. | | FERREIRA, D. N. M.; NOVELLI, V. M.; POLEZ, V. L. P.; CAMPOS, E.; NUNES, M. A.; MENDONÇA, R. S.; PIMENTA, L.; ASTUA, J. de F. The flat mite Brevipalpus phoenicis (Acari: Tenuipalpidae) genome project: update and perspectives. In: SYMPOSIUM OF THE EUROPEAN ASSOCIATION OF ACAROLOGISTS, 7., 2012, Viena. Program, abstracts, participantes... Viena: University of Natural Resources and life Sicences, 2012. p. 55 1 CD-ROM Anais Web.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 104. | | FERREIRA, D. N. M.; NOVELLI, V. M.; POLEZ, V. L. P.; CAMPOS, E.; NUNES, M. A.; MENDONÇA, R. S.; PIMENTA, L.; ASTUA, J. de F. The flat mite Brevipalpus phoenicis (Acari: Tenuipalpidae) genome project: update and perspectives. In: SYMPOSIUM OF THE EUROPEAN ASSOCIATION OF ACAROLOGISTS, 7., 2012, Viena. Abstracts... Vienna, Austria, 2012. Anais Web.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 106. | | NUNES, M. A.; MINEIRO, L. de C.; ROGERIO, L. A.; FERREIRA, L. M.; TASSI, A.; NOVELLI, V. M.; KITAJIMA, E. W.; ASTUA, J. de F. First Report of Brevipalpus papayensis as Vector of Coffee ringspot virus and Citrus leprosis virus C Plant Disease, v.102, n. 5, p.1046, May 2018. Disease Note.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 107. | | CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; TASSI, A. D.; BARGUIL, B. M.; BESERRA JUNIOR, J. E. A.; HARAKAVA, R.; KITAJIMA, E. W.; ASTUA, J. de F. First Report of Citrus Chlorotic Spot Virus Infecting the Succulent Plant Agave desmettiana. Plant Disease, v.103, n. 6, June 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 108. | | SAUVÊTRE, P.; VENIANT, E.; CROQ, G.; TASSI, A. D.; KITAJIMA, E. W.; CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; ASTUA, J. de F.; FERREIRA, D. N. M. First report of orchid fleck virus in the orchid collection of Jardin du Luxembourg, Paris, France. Plant Disease, v. 102, n. 12, p. 2670, 2018. Na publicação: J. Freitas-Astúa, D. Navia.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 109. | | COSTA-RODRIGUES, M. da; PEREIRA, L. R.; RAMOS-GONZÁLEZ, P. L.; TASSI, D.; LEÃO, I. G.; HARAKAVA, R.; KITAJIMA, E. W.; ASTUA, J. de F. First report of Passion fruit green spot virusinfecting hibiscus plants. New Disease Report, v.45, n.2, e12080, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 110. | | NOVELLI, V. M.; NUNES, M. A.; LATADO, R. R.; FERREIRA, D. N. M.; MENDONÇA, R. S.; BERGAMINI, M. P.; BASTIANEL, M.; ASTUA, J. de F. The genome size of Brevipalpus phoenicis estimated by flow cytometry: preliminary results. In: SYMPOSIUM OF THE EUROPEAN ASSOCIATION OF ACAROLOGISTS, 7., 2012, Viena. Abstracts... Vienna, Austria, 2012. Anais Web.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 111. | | NOVELLI, V. M.; NUNES, M. A.; LATADO, R. R.; FERREIRA, D. N. M.; R. S. MENDONÇA; BERGAMINI, M. P.; BASTIANEL, M.; ASTUA, J. de F. The genome size of Brevipalpus phoenicis estimated by flow cytometry: preliminary results. In: SYMPOSIUM OF THE EUROPEAN ASSOCIATION OF ACAROLOGISTS, 7., 2012, Viena. Program, abstracts, participantes... Viena: University of Natural Resources and life Sciences, 2012. p. 100-101 1 CD-ROM Anais Web.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 112. | | DORTA, S. DE O.; ATTÍLIO, L. B.; ZANARDI, O. Z.; LOPES, J. R. S.; MACHADO, M. A.; ASTUA, J. de F. Genetic transformation of 'Hamlin' and 'Valencia' sweet orange plants expressing the cry11A gene of Bacillus thuringiensis as an additional tool for the management of Diaphorina citri (Hemiptera: Liviidae). Journal of Biotechnolog, v.68, May, p.60-70, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 113. | | NUNES, M. A; NOVELLI, V. M.; CUNHA, B. A. da; SOARES, A. J.; MINEIRO, J. L. C. de; ASTUA, J. de F.; BASTIANEL, M. Survey of the citrus leprosis vector (Brevipalpus yothersi) and phytoseiids in spontaneous plants of an organic citrus orchard. Experimental and Applied Acarology, v. 82, p.199?209, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 114. | | NUNES, M. A.; LAMEIRO, P.; CUNHA, B. A. da; SOARES, A. J.; MINEIR0, J. L. DE C.; ASTUA, J. de F.; NOVELLI, V. M.; BASTIANEL, M. Survey of phytoseiids and mite-transmitted viruses in weeds from an organic sweet orange orchard in Brazil. In: SYMPOSIUM OF THE EUROPEAN ASSOCIATION OF ACAROLOGISTS, 7., 2012, Viena. Abstracts... Vienna, Austria, 2012. Anais Web.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 115. | | ARENA, G. D.; RAMOS-GONZÁLEZ, P. L.; ROGÉRIO, L.; CASTEEL, C.; FALK, B. W.; ASTUA, J. de F.; MACHADO, M. A. Uncovering the global plant gene expression and the role oh the viral proteins during citrus leprosis virus C infection. In: INTERNATIONAL CONFERENCE ON BIOTIC PLANT INTERACTION,5., 2017, XIAMEN, CHINA, 2017. Program & abstracts, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 116. | | ASTUA, J. de F.; DOCEMA, M. L.; STIPP, L. C. L.; HARAKAVA, R.; TAVANO, E. C. R.; ATTILIO, T. S. M. Transgenic "Hamlin" sweet orange expressing csd1 or d4e1 genes exhibits decreased susceptibility to huanglongbing and greater callose accumulation in the leaves. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 51., 2019, Recife. Os avanços da Fitopatologia na Era Genômica: anais. Recife: SBF: UFPRE/PPGF, 2019. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 117. | | NUNES, M. A.; OLIVEIRA, C. A. L. de; OLIVEIRA, M. L. de; KITAJIMA, E. W.; HILF, M. E.; GOTTWALD, T. R.; ASTUA, J. de F. Transmission of Citrus leprosis virus C by Brevipalpus phoenicis (Geijskes) to Alternative Host Plants Found in Citrus Orchards. Plant Disease, v. 96, n. 7., 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 118. | | NUNES, M. A.; LAMEIRO, P.; CALEGARIO, R. F.; BERGAMINI, M. P.; COERINI, L. F.; KITAJIMA, E. W.; BASTIANEL, M.; NOVELLI, V. M.; ASTUA, J. de F. Trapoeraba (Commelina benghalensis L.) como fonte de inóculo do vírus da leprose dos citros. Citrus Research & Technology, Cordeirópolis, v.33, n.1, p.1-9, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 119. | | RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; BANGUELA-CASTILLO, E.; TASSI, A. D.; RODRIGUES, M. da C.; KITAJIMA, E. W.; HARAKAVA, R.; ASTUA, J. de F. Unveiling the complete genome sequence of clerodendrum chlorotic spot virus, a putative dichorhavirus infecting ornamental plants. Archives of Virology, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 120. | | ASTUA, J. de F.; NOVELLI, V. M.; NUNES, M. A.; BASTIANEL, M.; ARENA, G. D.; RAMOS-GONZÁLEZ, P. L.; KITAJIMA, E. W.; MACHADO, M. A. Updates on leprosis research: what have we learned in the last decade? In: Conference of the International Organization of Citrus Virologists 19. Mpumalanga, South Africa, 2013. Program & Abstracts... Mpumalanga, South, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 179 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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