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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
23/04/2019 |
Data da última atualização: |
06/12/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
DORTA, S. de O.; BALBINOTTE, J.; MONNERAT, R.; LOPES, J. R. S.; CUNHA, T. da; ZANARDI, O. Z.; MIRANDA, M. P. de; MACHADO, M. A.; ASTUA, J. de F. |
Afiliação: |
SILVIA DE OLIVEIRA DORTA, ESALQ; JULIANA BALBINOTTE, Centro de Citricultura Sylvio Moreira; ROSE MONNERAT, ESALQ; JOAO ROBERTO SPOTTI LOPES, Centro de Citricultura Sylvio Moreira; TATIANE DA CUNHA, ESALQ; ODIMAR ZANUZO ZANARDI, Fundecitrus; MARCELO PEDREIRA DE MIRANDA, Fundecitrus; MARCOS ANTONIO MACHADO, Centro de Citricultura Sylvio Moreira; JULIANA DE FREITAS ASTUA, CNPMF. |
Título: |
Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Insect Science, p.1-12, 2019. |
ISSN: |
1744-7917 |
Idioma: |
Inglês |
Conteúdo: |
Bacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus. MenosBacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for... Mostrar Tudo |
Thesagro: |
Doença de Planta; Fruta Cítrica. |
Thesaurus Nal: |
Citrus; Diaphorina citri; Plant diseases and disorders. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/196193/1/Dorta-et-al-2019-Insect-Science.pdf
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Marc: |
LEADER 02385naa a2200289 a 4500 001 2108421 005 2019-12-06 008 2019 bl uuuu u00u1 u #d 022 $a1744-7917 100 1 $aDORTA, S. de O. 245 $aSelection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera$bLiviidae) nymphs.$h[electronic resource] 260 $c2019 520 $aBacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus. 650 $aCitrus 650 $aDiaphorina citri 650 $aPlant diseases and disorders 650 $aDoença de Planta 650 $aFruta Cítrica 700 1 $aBALBINOTTE, J. 700 1 $aMONNERAT, R. 700 1 $aLOPES, J. R. S. 700 1 $aCUNHA, T. da 700 1 $aZANARDI, O. Z. 700 1 $aMIRANDA, M. P. de 700 1 $aMACHADO, M. A. 700 1 $aASTUA, J. de F. 773 $tInsect Science, p.1-12, 2019.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
Biblioteca(s): |
Embrapa Trigo. |
Data corrente: |
01/07/2019 |
Data da última atualização: |
01/07/2019 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
BAYER, V. M. L.; SCHEUER, P. M.; MATTIONI, B.; MIRANDA, M. Z. de; DE FRANCISCO, A. |
Afiliação: |
VALÉRIA MARIA LIMBERGER BAYER; PATRÍCIA MATOS SCHEUER; BRUNA MATTIONI; MARTHA ZAVARIZ DE MIRANDA, CNPT; ALICIA DE FRANCISCO. |
Título: |
Treinamento para perfil de textura sensorial em pães. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Higiene Alimentar, v. 33, n. 288/289, p. 1521-1525, abr./maio 2019. |
Idioma: |
Português |
Notas: |
Edição dos Anais do IX Congresso Latino Americano, XV Congresso Brasileiro de Higienistas de Alimentos e VII Encontro do Sistema Brasileiro de Inspeção de Produtos de Origem Animal, Maceió, abr./maio 2019. |
Thesagro: |
Farinha de Trigo; Panificação. |
Thesaurus NAL: |
Bakery industry; Breads; Wheat flour. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198951/1/ID44596-2019v33n288p1521HigieneAlimentar.pdf
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Marc: |
LEADER 00838nam a2200217 a 4500 001 2110217 005 2019-07-01 008 2019 bl uuuu u00u1 u #d 100 1 $aBAYER, V. M. L. 245 $aTreinamento para perfil de textura sensorial em pães.$h[electronic resource] 260 $aHigiene Alimentar, v. 33, n. 288/289, p. 1521-1525, abr./maio 2019.$c2019 500 $aEdição dos Anais do IX Congresso Latino Americano, XV Congresso Brasileiro de Higienistas de Alimentos e VII Encontro do Sistema Brasileiro de Inspeção de Produtos de Origem Animal, Maceió, abr./maio 2019. 650 $aBakery industry 650 $aBreads 650 $aWheat flour 650 $aFarinha de Trigo 650 $aPanificação 700 1 $aSCHEUER, P. M. 700 1 $aMATTIONI, B. 700 1 $aMIRANDA, M. Z. de 700 1 $aDE FRANCISCO, A.
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