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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/03/2016 |
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Data da última atualização: |
18/03/2016 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ASSUNÇÃO, C. M.; MENDES, V. R. A.; PEREIRA, J. V. T. do N.; SOUZA, E. D.; GOMES, E. T.; CARVALHO, B. C. de; VIANA, J. H. M.; CAMARGO, L. S. de A. |
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Afiliação: |
CAROLINA MARINHO DE ASSUNÇÃO, UFJF; VIVIAN RACHEL ARAÚJO MENDES, UFV; JHONATA VIEIRA TAVARES DO NASCIMENTO PEREIRA, UFES; ELIZA DINIZ DE SOUZA, UFES; ELIZABETE TEIXEIRA GOMES, UFRPE; BRUNO CAMPOS DE CARVALHO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Comparação da eficiência de diferentes diluentes na criopreservação de sêmen de touros jovens da raça Holandesa. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO INTERNACIONAL DO LEITE, 13., 2015, Porto Alegre. Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. |
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Páginas: |
4 p. |
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Idioma: |
Português |
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Palavras-Chave: |
Botu-bov; Citrato-gema de ovo; Congelação; Touros; Tris-gema de ovo. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 00846naa a2200265 a 4500
001 2040814
005 2016-03-18
008 2015 bl uuuu u00u1 u #d
100 1 $aASSUNÇÃO, C. M.
245 $aComparação da eficiência de diferentes diluentes na criopreservação de sêmen de touros jovens da raça Holandesa.
260 $c2015
300 $a4 p.
653 $aBotu-bov
653 $aCitrato-gema de ovo
653 $aCongelação
653 $aTouros
653 $aTris-gema de ovo
700 1 $aMENDES, V. R. A.
700 1 $aPEREIRA, J. V. T. do N.
700 1 $aSOUZA, E. D.
700 1 $aGOMES, E. T.
700 1 $aCARVALHO, B. C. de
700 1 $aVIANA, J. H. M.
700 1 $aCAMARGO, L. S. de A.
773 $tIn: CONGRESSO INTERNACIONAL DO LEITE, 13., 2015, Porto Alegre. Anais... Juiz de Fora: Embrapa Gado de Leite, 2015.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
14/11/2022 |
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Data da última atualização: |
14/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; TASSI, A. D.; CALEGARIO, R. F.; HARAKAVA, R.; NOME, C F.; KITAJIMA, E. W.; ASTUA, J. de F. |
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Afiliação: |
PEDRO L. RAMOS-GONZÁLEZ, Instituto Biológico de São Paulo; CAMILA CHABI-JESUS, Instituto Biológico de São Paulo; ALINE D. TASSI, Instituto Biológico de São Paulo; RENATA FAIER CALEGARIO, Universidade Federal do Paraná; RICARDO HARAKAVA, Instituto Biológico de São Paulo; CLAUDIA F. NOME, Instituto Nacional de Tecnología Agropecuaria; ELLIOT W. KITAJIMA, Escola Superior de Agricultura Luiz de Queiroz; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
A Novel Lineage of Cile-Like Viruses Discloses the Phylogenetic Continuum Across the Family Kitaviridae. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Frontiers in Microbiology, v.28, n.13, 836076, March 2022. |
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DOI: |
https://doi.org/10.3389/fmicb.2022.836076 |
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Idioma: |
Francês |
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Conteúdo: |
An increasing number of plant species have been recognized or considered likely reservoirs of viruses transmitted by Brevipalpus mites. A tiny fraction of these viruses, primarily those causing severe economic burden to prominent crops, have been fully characterized. In this study, based on high-throughput sequencing, transmission electron microscopy analyses of virions in plant-infected tissues, viral transmission experiments, and the morphoanatomical identification of the involved Brevipalpus mites, we describe molecular and biological features of viruses representing three new tentative species of the family Kitaviridae. The genomes of Solanum violifolium ringspot virus (SvRSV, previously partially characterized), Ligustrum chlorotic spot virus (LigCSV), and Ligustrum leprosis virus (LigLV) have five open reading frames (ORFs) > 500 nts, two distributed in RNA1 and three in RNA2. RNA1 of these three viruses display the same genomic organization found in RNA1 of typical cileviruses, while their RNA2 are shorter, possessing only orthologs of genes p61, p32, and p24. LigCSV and LigLV are more closely related to each other than to SvRSV, but the identities between their genomic RNAs were lower than 70%. In gene-by-gene comparisons, ORFs from LigCSV and LigLV had the highest sequence identity values (nt sequences: 70?76% and deduced amino acid sequences: 74?83%). The next higher identity values were with ORFs from typical cileviruses, with values below 66%. Virions of LigLV (? 40 nm × 55 nm) and LigCSV (? 54 nm × 66 nm) appear almost spherical, contrasting with the bacilliform shape of SvRSV virions (? 47 nm × 101 nm). Mites collected from the virus-infected plants were identified as Brevipalpus papayensis, B. tucuman, and B. obovatus. Viruliferous B. papayensis mites successfully transmitted LigCSV to Arabidopsis thaliana. SvRSV, LigCSV, and LigLV seem to represent novel sub-lineages of kitaviruses that descent on parallel evolutionary branches from a common ancestor shared with the tentative cile-like virus hibiscus yellow blotch virus and typical cileviruses. Biological and molecular data, notably, the phylogenetic reconstruction based on the RdRp proteins in which strong support for monophyly of the family Kitaviridae is observed, mark an advance in the understanding of kitavirids. MenosAn increasing number of plant species have been recognized or considered likely reservoirs of viruses transmitted by Brevipalpus mites. A tiny fraction of these viruses, primarily those causing severe economic burden to prominent crops, have been fully characterized. In this study, based on high-throughput sequencing, transmission electron microscopy analyses of virions in plant-infected tissues, viral transmission experiments, and the morphoanatomical identification of the involved Brevipalpus mites, we describe molecular and biological features of viruses representing three new tentative species of the family Kitaviridae. The genomes of Solanum violifolium ringspot virus (SvRSV, previously partially characterized), Ligustrum chlorotic spot virus (LigCSV), and Ligustrum leprosis virus (LigLV) have five open reading frames (ORFs) > 500 nts, two distributed in RNA1 and three in RNA2. RNA1 of these three viruses display the same genomic organization found in RNA1 of typical cileviruses, while their RNA2 are shorter, possessing only orthologs of genes p61, p32, and p24. LigCSV and LigLV are more closely related to each other than to SvRSV, but the identities between their genomic RNAs were lower than 70%. In gene-by-gene comparisons, ORFs from LigCSV and LigLV had the highest sequence identity values (nt sequences: 70?76% and deduced amino acid sequences: 74?83%). The next higher identity values were with ORFs from typical cileviruses, with values below 66%. Virions of LigLV (?... Mostrar Tudo |
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Thesagro: |
Planta Ornamental; Vírus. |
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Thesaurus NAL: |
Brevipalpus; Ornamental plants; Virion. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148258/1/fmicb-13-836076.pdf
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Marc: |
LEADER 03166naa a2200277 a 4500
001 2148258
005 2022-11-14
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fmicb.2022.836076$2DOI
100 1 $aRAMOS-GONZÁLEZ, P. L.
245 $aA Novel Lineage of Cile-Like Viruses Discloses the Phylogenetic Continuum Across the Family Kitaviridae.$h[electronic resource]
260 $c2022
520 $aAn increasing number of plant species have been recognized or considered likely reservoirs of viruses transmitted by Brevipalpus mites. A tiny fraction of these viruses, primarily those causing severe economic burden to prominent crops, have been fully characterized. In this study, based on high-throughput sequencing, transmission electron microscopy analyses of virions in plant-infected tissues, viral transmission experiments, and the morphoanatomical identification of the involved Brevipalpus mites, we describe molecular and biological features of viruses representing three new tentative species of the family Kitaviridae. The genomes of Solanum violifolium ringspot virus (SvRSV, previously partially characterized), Ligustrum chlorotic spot virus (LigCSV), and Ligustrum leprosis virus (LigLV) have five open reading frames (ORFs) > 500 nts, two distributed in RNA1 and three in RNA2. RNA1 of these three viruses display the same genomic organization found in RNA1 of typical cileviruses, while their RNA2 are shorter, possessing only orthologs of genes p61, p32, and p24. LigCSV and LigLV are more closely related to each other than to SvRSV, but the identities between their genomic RNAs were lower than 70%. In gene-by-gene comparisons, ORFs from LigCSV and LigLV had the highest sequence identity values (nt sequences: 70?76% and deduced amino acid sequences: 74?83%). The next higher identity values were with ORFs from typical cileviruses, with values below 66%. Virions of LigLV (? 40 nm × 55 nm) and LigCSV (? 54 nm × 66 nm) appear almost spherical, contrasting with the bacilliform shape of SvRSV virions (? 47 nm × 101 nm). Mites collected from the virus-infected plants were identified as Brevipalpus papayensis, B. tucuman, and B. obovatus. Viruliferous B. papayensis mites successfully transmitted LigCSV to Arabidopsis thaliana. SvRSV, LigCSV, and LigLV seem to represent novel sub-lineages of kitaviruses that descent on parallel evolutionary branches from a common ancestor shared with the tentative cile-like virus hibiscus yellow blotch virus and typical cileviruses. Biological and molecular data, notably, the phylogenetic reconstruction based on the RdRp proteins in which strong support for monophyly of the family Kitaviridae is observed, mark an advance in the understanding of kitavirids.
650 $aBrevipalpus
650 $aOrnamental plants
650 $aVirion
650 $aPlanta Ornamental
650 $aVírus
700 1 $aCHABI-JESUS, C.
700 1 $aTASSI, A. D.
700 1 $aCALEGARIO, R. F.
700 1 $aHARAKAVA, R.
700 1 $aNOME, C F.
700 1 $aKITAJIMA, E. W.
700 1 $aASTUA, J. de F.
773 $tFrontiers in Microbiology$gv.28, n.13, 836076, March 2022.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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