| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Instrumentação. |
|
Data corrente: |
09/09/2003 |
|
Data da última atualização: |
01/03/2010 |
|
Autoria: |
ASSIS, O. B. G.; CLARO, L. C. |
|
Afiliação: |
Embrapa Instrumentação Agropecuária, São Carlos, SP. |
|
Título: |
Immobilized lysozyme protein on fibrous medium: preliminary results for microfiltration applications. |
|
Ano de publicação: |
2003 |
|
Fonte/Imprenta: |
Electronic Journal of Biotechnology, Valparaiso, v. 6, n. 2, p. 14-20, 2003. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The protein lysozyme was deposited onto a permeable support comprising chemically functionalized glass fiber. The main objective of this study was to set a stable organic net with no effect on the medium bed permeability and a preliminary test the activity of this enzyme under immobilized conditions. The film formation is followed by atomic force microscopy (AFM) surface imaging. The effect on the bacteria Escherichia coli was tested using a simple microfiltration column. The filtration results pointed around 75% removal of bacteria in the effluent when compared to the influent concretation. The removal mechanism is assumed as being essentially due biointeraction. The surface polarity characteristics of the formed film were also considered as playing an important role, suggesting an electrostatic interaction mechanism in the microorganism removal. |
|
Palavras-Chave: |
AFM; Enzyme immobilization; Microscopia de força atômica; Packed bed column; Proteína lysozyme; Surface analysis. |
|
Thesagro: |
Escherichia Coli. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01531naa a2200217 a 4500
001 1024779
005 2010-03-01
008 2003 bl --- 0-- u #d
100 1 $aASSIS, O. B. G.
245 $aImmobilized lysozyme protein on fibrous medium$bpreliminary results for microfiltration applications.
260 $c2003
520 $aThe protein lysozyme was deposited onto a permeable support comprising chemically functionalized glass fiber. The main objective of this study was to set a stable organic net with no effect on the medium bed permeability and a preliminary test the activity of this enzyme under immobilized conditions. The film formation is followed by atomic force microscopy (AFM) surface imaging. The effect on the bacteria Escherichia coli was tested using a simple microfiltration column. The filtration results pointed around 75% removal of bacteria in the effluent when compared to the influent concretation. The removal mechanism is assumed as being essentially due biointeraction. The surface polarity characteristics of the formed film were also considered as playing an important role, suggesting an electrostatic interaction mechanism in the microorganism removal.
650 $aEscherichia Coli
653 $aAFM
653 $aEnzyme immobilization
653 $aMicroscopia de força atômica
653 $aPacked bed column
653 $aProteína lysozyme
653 $aSurface analysis
700 1 $aCLARO, L. C.
773 $tElectronic Journal of Biotechnology, Valparaiso$gv. 6, n. 2, p. 14-20, 2003.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Instrumentação (CNPDIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 5 | |
| Registros recuperados : 5 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
