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Registro Completo |
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
18/03/2016 |
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Data da última atualização: |
21/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOMES, G. L. B.; SANTOS, R. C. dos; ALBUQUERQUE, F. A. de; ARRIEL, N. H. C.; LIMA, L. M. de. |
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Afiliação: |
GÉSSICA LAIZE BERTO GOMES, UEPB; ROSEANE CAVALCANTI DOS SANTOS, CNPA; FABIO AQUINO DE ALBUQUERQUE, CNPA; NAIR HELENA CASTRO ARRIEL, CNPA; LIZIANE MARIA DE LIMA, CNPA. |
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Título: |
Bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae (Hübner) (Lepidoptera: Pyralidae). |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
African Journal of Agricultural Research, v. 10. n. 35, p. 3517-3524, Aug. 2015. |
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ISSN: |
1991-637X |
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Idioma: |
Inglês |
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Conteúdo: |
Biochemical and feeding assays were performed in order to assess the bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae. The biochemical assays were based on the ability of protease inhibitors (PI) present in sesame seeds to inhibit bovine pancreatic trypsin and digestive enzymes of insect larvae. The stability of the inhibitors was also assessed at different pH and temperature. The feeding bioassays were carried out in plates containing second instar larvae fed on diet with sesame protein extract. It was observed that all genotypes presented inhibition when tested with BPT, ranging from 51 to 90%, among them BRS Seda, CNPA G3 and CNPA G4 showed average of 69% of inhibitory activity for intestinal homogenate. The FII-fractions in these three genotypes showed thermal and pH stabilities at 40°C and 8.5, respectively. In feeding bioassay, P. interpunctella larvae were susceptible to SPCE of all three genotypes, revealing average mortality of 74% to SPCE and 45% to FII-fractions. Among all genotypes, BRS Seda was more effective to inhibition of digestive enzymes of insects and considered the more suitable genotype for further using in sesame breeding program to resistance to P. interpunctella. |
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Palavras-Chave: |
Seed; Sesamum indicum L; Tripisina. |
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Thesagro: |
Genótipo; Gergelim; Plodia interpunctella; Semente. |
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Thesaurus Nal: |
Lepidoptera. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141430/1/Bioactivity-of-trypsin-inhibitors-....pdf
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Marc: |
LEADER 02090naa a2200277 a 4500
001 2041438
005 2016-03-21
008 2015 bl uuuu u00u1 u #d
022 $a1991-637X
100 1 $aGOMES, G. L. B.
245 $aBioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae (Hübner) (Lepidoptera$bPyralidae).$h[electronic resource]
260 $c2015
520 $aBiochemical and feeding assays were performed in order to assess the bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae. The biochemical assays were based on the ability of protease inhibitors (PI) present in sesame seeds to inhibit bovine pancreatic trypsin and digestive enzymes of insect larvae. The stability of the inhibitors was also assessed at different pH and temperature. The feeding bioassays were carried out in plates containing second instar larvae fed on diet with sesame protein extract. It was observed that all genotypes presented inhibition when tested with BPT, ranging from 51 to 90%, among them BRS Seda, CNPA G3 and CNPA G4 showed average of 69% of inhibitory activity for intestinal homogenate. The FII-fractions in these three genotypes showed thermal and pH stabilities at 40°C and 8.5, respectively. In feeding bioassay, P. interpunctella larvae were susceptible to SPCE of all three genotypes, revealing average mortality of 74% to SPCE and 45% to FII-fractions. Among all genotypes, BRS Seda was more effective to inhibition of digestive enzymes of insects and considered the more suitable genotype for further using in sesame breeding program to resistance to P. interpunctella.
650 $aLepidoptera
650 $aGenótipo
650 $aGergelim
650 $aPlodia interpunctella
650 $aSemente
653 $aSeed
653 $aSesamum indicum L
653 $aTripisina
700 1 $aSANTOS, R. C. dos
700 1 $aALBUQUERQUE, F. A. de
700 1 $aARRIEL, N. H. C.
700 1 $aLIMA, L. M. de
773 $tAfrican Journal of Agricultural Research$gv. 10. n. 35, p. 3517-3524, Aug. 2015.
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Registro original: |
Embrapa Algodão (CNPA) |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
18/08/2011 |
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Data da última atualização: |
18/08/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SANTOS, S. N.; OLIVEIRA, L. K. X.; MELO, I. S. de; VELOSO, E. da S.; ROQUE, M. R. de A. |
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Afiliação: |
SUIKINAI NOBRE SANTOS, ESALQ; LIDIANE KARLA XISTO OLIVEIRA, INSTITUTO FEDERAL BAIANO; ITAMAR SOARES DE MELO, CNPMA; EUDES DA SILVA VELOZO, UFBA; MILTON RICARDO DE ABREU ROQUE, UFBA. |
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Título: |
Antifungal activity of bacterial strains from the rhizosphere of Stachytarpheta crassifolia. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
African Journal of Biotechnology, Nairobi, v. 10, n. 25, p. 4996-5000, jun. 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
This study evaluated the antifungal action of biomolecules produced from the secondary metabolism of bacterial strains found in the rhizosphere of semi arid plants against human pathogenic Candida albicans. Crude extracts were obtained using ethyl acetate as an organic solvent and the bioactivity was assessed with a bioautography technique. The results showed that bacterial strains, Alcaligenes faecalis MRbS12 and Bacillus cereus MRbS26, had compounds with antifungal bioactivity. The largest inhibition zones for both compounds were located on spots with Rf values below 0.500, indicating that the molecules possibly had polar characteristics. These results suggested that microorganisms found in the environment could have bioprospecting potential. |
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Palavras-Chave: |
Antifungal activity; Bioautoghaphy; Biomolecules. |
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Thesagro: |
Bactéria; Candida albicans; Controle biológico; Rizosfera. |
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Thesaurus NAL: |
Alcaligenes faecalis; Antifungal agents; Bacillus cereus; Biological control; Rhizobiales. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/40033/1/2011AP16.pdf
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Marc: |
LEADER 01690naa a2200313 a 4500
001 1898255
005 2011-08-18
008 2011 bl uuuu u00u1 u #d
100 1 $aSANTOS, S. N.
245 $aAntifungal activity of bacterial strains from the rhizosphere of Stachytarpheta crassifolia.$h[electronic resource]
260 $c2011
520 $aThis study evaluated the antifungal action of biomolecules produced from the secondary metabolism of bacterial strains found in the rhizosphere of semi arid plants against human pathogenic Candida albicans. Crude extracts were obtained using ethyl acetate as an organic solvent and the bioactivity was assessed with a bioautography technique. The results showed that bacterial strains, Alcaligenes faecalis MRbS12 and Bacillus cereus MRbS26, had compounds with antifungal bioactivity. The largest inhibition zones for both compounds were located on spots with Rf values below 0.500, indicating that the molecules possibly had polar characteristics. These results suggested that microorganisms found in the environment could have bioprospecting potential.
650 $aAlcaligenes faecalis
650 $aAntifungal agents
650 $aBacillus cereus
650 $aBiological control
650 $aRhizobiales
650 $aBactéria
650 $aCandida albicans
650 $aControle biológico
650 $aRizosfera
653 $aAntifungal activity
653 $aBioautoghaphy
653 $aBiomolecules
700 1 $aOLIVEIRA, L. K. X.
700 1 $aMELO, I. S. de
700 1 $aVELOSO, E. da S.
700 1 $aROQUE, M. R. de A.
773 $tAfrican Journal of Biotechnology, Nairobi$gv. 10, n. 25, p. 4996-5000, jun. 2011.
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Embrapa Meio Ambiente (CNPMA) |
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