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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria Tropical; Embrapa Gado de Leite. |
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Data corrente: |
09/12/2019 |
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Data da última atualização: |
09/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ANDRADE, A. P. C. DE; BORGES, M. DE F.; EVÂNIA ALTINA TEIXEIRA DE FIGUEIREDO; ARCURI, E. F. |
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Afiliação: |
ANA PAULA COLARES DE ANDRADE, Universidade Federal do Ceará, Departamento de Engenharia de Alimentos; MARIA DE FÁTIMA BORGES, Embrapa Agroindústria Tropical, Doutora, Pesquisadora aposentada.; EVÂNIA ALTINA TEIXEIRA DE FIGUEIREDO, Universidade Federal do Ceará, Departamento de Engenharia de Alimentos; EDNA FROEDER ARCURI, CNPGL. |
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Título: |
Diversity of Staphylococcus coagulase: positive and negative strains of coalho cheese and detection of enterotoxin encoding genes. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Boletim Centro de Pesquisa de Processamento de Alimentos, Curitiba, v. 36, n. 1, p. 1-9, jan./jun. 2019. |
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DOI: |
doi.org/10.5380/bceppa.v36i1.57553 |
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Idioma: |
Inglês |
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Conteúdo: |
Three hundred samples of coalho cheese, from 15 diff erent brands, seven handmade and eight industrialized, were evaluated in relation to the contamination profi le by Staphylococcus coagulase-positive and negative and the occurrence of staphylococcal enterotoxin encoding genes. Two hundred and eight isolates of Staphylococcus sp. were subjected to phenotypic identifi cation and 95 were subjected to genotypic identifi cation through femA gene research and detection of genes (sea, seb, sec, sed, see, seg, seh, sei and sej) encoding enterotoxins, using the polymerase chain reaction technique (PCR). A total of 14 species of Staphylococcus were identifi ed, of which three were coagulase-positive and eleven negative, especially: S. aureus, S. xylosus, S. cohnni spp. cohnii, S. saprophyticus, S. epidermidis, S. hyicus, S. lentus, S. sciuri, S. cohnii spp. urealyticus, S. haemolyticus, S. chromogenes, S. lugdunensis, S. hominis e S. intermedius. In all the samples of handmade coalho cheese there was a prevalence of S. aureus; while at industrial samples S. xylosus (87.5%) and S. cohnii spp cohnii (50%) were predominant. The presence of the femA gene was detected in 95% (38/40) isolates of positive Staphylococcus coagulase and 16.4% (9/55) of coagulasenegative isolates. Among the enterotoxin encoding genes evaluated, there was prevalence of the seh gene (53.2%) in coagulase-positive strains and of the sec gene (46.8%) in coagulase-negative strains. The results suggest a re-evaluation of the Brazilian microbiological standards in relation to the genus Staphylococcus in foods. MenosThree hundred samples of coalho cheese, from 15 diff erent brands, seven handmade and eight industrialized, were evaluated in relation to the contamination profi le by Staphylococcus coagulase-positive and negative and the occurrence of staphylococcal enterotoxin encoding genes. Two hundred and eight isolates of Staphylococcus sp. were subjected to phenotypic identifi cation and 95 were subjected to genotypic identifi cation through femA gene research and detection of genes (sea, seb, sec, sed, see, seg, seh, sei and sej) encoding enterotoxins, using the polymerase chain reaction technique (PCR). A total of 14 species of Staphylococcus were identifi ed, of which three were coagulase-positive and eleven negative, especially: S. aureus, S. xylosus, S. cohnni spp. cohnii, S. saprophyticus, S. epidermidis, S. hyicus, S. lentus, S. sciuri, S. cohnii spp. urealyticus, S. haemolyticus, S. chromogenes, S. lugdunensis, S. hominis e S. intermedius. In all the samples of handmade coalho cheese there was a prevalence of S. aureus; while at industrial samples S. xylosus (87.5%) and S. cohnii spp cohnii (50%) were predominant. The presence of the femA gene was detected in 95% (38/40) isolates of positive Staphylococcus coagulase and 16.4% (9/55) of coagulasenegative isolates. Among the enterotoxin encoding genes evaluated, there was prevalence of the seh gene (53.2%) in coagulase-positive strains and of the sec gene (46.8%) in coagulase-negative strains. The results suggest a re-evaluatio... Mostrar Tudo |
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Palavras-Chave: |
ENTEROTOXINAS ESTAFILOCÓCICAS; ENVENENAMENTO ALIMENTAR; LACTICÍNIOS. |
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Thesagro: |
Intoxicação Alimentar. |
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Thesaurus Nal: |
Dairy products; Enterotoxins; Staphylococcal intoxication. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/206483/1/ART19040.pdf
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Marc: |
LEADER 02514naa a2200253 a 4500
001 2116347
005 2019-12-09
008 2019 bl uuuu u00u1 u #d
024 7 $adoi.org/10.5380/bceppa.v36i1.57553$2DOI
100 1 $aANDRADE, A. P. C. DE
245 $aDiversity of Staphylococcus coagulase$bpositive and negative strains of coalho cheese and detection of enterotoxin encoding genes.$h[electronic resource]
260 $c2019
520 $aThree hundred samples of coalho cheese, from 15 diff erent brands, seven handmade and eight industrialized, were evaluated in relation to the contamination profi le by Staphylococcus coagulase-positive and negative and the occurrence of staphylococcal enterotoxin encoding genes. Two hundred and eight isolates of Staphylococcus sp. were subjected to phenotypic identifi cation and 95 were subjected to genotypic identifi cation through femA gene research and detection of genes (sea, seb, sec, sed, see, seg, seh, sei and sej) encoding enterotoxins, using the polymerase chain reaction technique (PCR). A total of 14 species of Staphylococcus were identifi ed, of which three were coagulase-positive and eleven negative, especially: S. aureus, S. xylosus, S. cohnni spp. cohnii, S. saprophyticus, S. epidermidis, S. hyicus, S. lentus, S. sciuri, S. cohnii spp. urealyticus, S. haemolyticus, S. chromogenes, S. lugdunensis, S. hominis e S. intermedius. In all the samples of handmade coalho cheese there was a prevalence of S. aureus; while at industrial samples S. xylosus (87.5%) and S. cohnii spp cohnii (50%) were predominant. The presence of the femA gene was detected in 95% (38/40) isolates of positive Staphylococcus coagulase and 16.4% (9/55) of coagulasenegative isolates. Among the enterotoxin encoding genes evaluated, there was prevalence of the seh gene (53.2%) in coagulase-positive strains and of the sec gene (46.8%) in coagulase-negative strains. The results suggest a re-evaluation of the Brazilian microbiological standards in relation to the genus Staphylococcus in foods.
650 $aDairy products
650 $aEnterotoxins
650 $aStaphylococcal intoxication
650 $aIntoxicação Alimentar
653 $aENTEROTOXINAS ESTAFILOCÓCICAS
653 $aENVENENAMENTO ALIMENTAR
653 $aLACTICÍNIOS
700 1 $aBORGES, M. DE F.
700 1 $aEVÂNIA ALTINA TEIXEIRA DE FIGUEIREDO
700 1 $aARCURI, E. F.
773 $tBoletim Centro de Pesquisa de Processamento de Alimentos, Curitiba$gv. 36, n. 1, p. 1-9, jan./jun. 2019.
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Registro original: |
Embrapa Agroindústria Tropical (CNPAT) |
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| 1. |  | MEINKE, H.; BASTIAANS, L.; BOUMAN, B.; DINGKUHN, M.; GAYDON, D.; HASEGAWA, T.; HEINEMANN, A. B.; KIEPE, P.; LAFARGE, T.; LUQUET, D.; MASOOD, A.; OORT, P. van; RODENBURG, J.; YAN, J.; YIN, X. An international collaborative research network helps to design climate robust rice systems. In: MARCO SYMPOSIUM 2009: WORKSHOP 2: CROP PRODUCTION UNDER HEAT STRESS: MONITORING, IMPACT ASSESSMENT AND ADAPTATION, 2009, Tsukuba. Challenges for agro-environmental research in Monsoon Asia. Tsukuba: National Institute for AGr-Environmental Sciences, 2009. 11 p.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
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