| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agrobiologia. |
|
Data corrente: |
09/06/2021 |
|
Data da última atualização: |
09/11/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
PESSOA, D. D. V.; SANTOS, C. M. dos; VIDAL, M. S.; BALDANI, J. I.; TADRA-SFEIR, M. Z.; SOUZA, E. M. de; ARAUJO, J. L. S. de. |
|
Afiliação: |
DANIELLA DUARTE VILLARINHO PESSOA, UFRRJ; CARLOS MAGNO DOS SANTOS, UFRRJ; MARCIA SOARES VIDAL, CNPAB; JOSE IVO BALDANI, CNPAB; MICHELLE ZIBETTI TADRA-SFEIR, UFPR; EMANUEL MALTEMPI DE SOUZA, UFPR; JEAN LUIZ SIMOES DE ARAUJO, CNPAB. |
|
Título: |
Herbaspirillum seropedicae strain HRC54 expression profile in response to sugarcane apoplastic fluid. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
3 Biotech, v. 11, Article number 292, 2021. |
|
ISSN: |
2190-5738 |
|
DOI: |
10.1007/s13205-021-02848-y |
|
Idioma: |
Inglês |
|
Conteúdo: |
Bacterial transcriptome profiling in the presence of plant fluids or extracts during microbial growth may provide relevant information on plant?bacteria interactions. Here, RNA sequencing (RNA-Seq) was used to determine the transcriptomic profile of Herbaspirillum seropedicae strain HRC54 at the early stages of response to sugarcane apoplastic fluid. Differentially expressed gene (DEG) analysis was performed using the DESeq2 and edgeR packages, followed by functional annotation using Blast2GO and gene ontology enrichment analysis using the COG and KEGG databases. After 2 h of sugarcane apoplastic fluid addition to the H. seropedicae HRC54 culture, respectively, 44 and 45 genes were upregulated and downregulated. These genes were enriched in bacterial metabolism (e.g., oxidoreductase and transferase), ABC transporters, motility, secretion systems, and signal transduction. RNA-Seq expression profiles of 12 genes identified in data analyses were verified by RT-qPCR. The results suggested that H. seropedicae HRC54 recognized sugarcane apoplastic fluid as the host signal, and some DEGs were closely involved at the early stages of the establishment of plant?bacteria interactions. |
|
Palavras-Chave: |
Plant bacteria interactions. |
|
Thesaurus Nal: |
Sugarcane. |
|
Categoria do assunto: |
S Ciências Biológicas |
|
Marc: |
LEADER 01927naa a2200241 a 4500
001 2132254
005 2021-11-09
008 2021 bl uuuu u00u1 u #d
022 $a2190-5738
024 7 $a10.1007/s13205-021-02848-y$2DOI
100 1 $aPESSOA, D. D. V.
245 $aHerbaspirillum seropedicae strain HRC54 expression profile in response to sugarcane apoplastic fluid.$h[electronic resource]
260 $c2021
520 $aBacterial transcriptome profiling in the presence of plant fluids or extracts during microbial growth may provide relevant information on plant?bacteria interactions. Here, RNA sequencing (RNA-Seq) was used to determine the transcriptomic profile of Herbaspirillum seropedicae strain HRC54 at the early stages of response to sugarcane apoplastic fluid. Differentially expressed gene (DEG) analysis was performed using the DESeq2 and edgeR packages, followed by functional annotation using Blast2GO and gene ontology enrichment analysis using the COG and KEGG databases. After 2 h of sugarcane apoplastic fluid addition to the H. seropedicae HRC54 culture, respectively, 44 and 45 genes were upregulated and downregulated. These genes were enriched in bacterial metabolism (e.g., oxidoreductase and transferase), ABC transporters, motility, secretion systems, and signal transduction. RNA-Seq expression profiles of 12 genes identified in data analyses were verified by RT-qPCR. The results suggested that H. seropedicae HRC54 recognized sugarcane apoplastic fluid as the host signal, and some DEGs were closely involved at the early stages of the establishment of plant?bacteria interactions.
650 $aSugarcane
653 $aPlant bacteria interactions
700 1 $aSANTOS, C. M. dos
700 1 $aVIDAL, M. S.
700 1 $aBALDANI, J. I.
700 1 $aTADRA-SFEIR, M. Z.
700 1 $aSOUZA, E. M. de
700 1 $aARAUJO, J. L. S. de
773 $t3 Biotech$gv. 11, Article number 292, 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 163 | |
| 9. |  | SILVA, F. V.; ARAÚJO, J. L. S. de; RUMJANEK, N. G.; SILVA JÚNIOR, J. P. Caracterização de Bradyrhizobium da região amazônica por ardra e sequenciamento do 16S rDNA. In: ENCONTRO NACIONAL DE MICROBIOLOGIA AMBIENTAL, 11., SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 10., 2008, Fortaleza. Resumos... Fortaleza, 2008.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Agrobiologia. |
|   |
| 13. | | BAHIA, P. P.; ARAUJO, J. L. S. de; SOARES, L. H. de B.; REIS, V. M. Desenvolvimento de bioprocesso para produção de inoculante de cana-de-açúcar através do co-cultivo de duas espécies de herbaspirillum In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., FERTBIO 2016. Goiânia. Rumo aos novos desafios: anais. Goiânia: UFG, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
| |
| 20. | | PESSOA, D. D. V.; VIDAL, M. S.; BALDANI, J. I.; ARAUJO, J. L. S. de. The effect of sugarcane apoplastic fluidon the global gene expression pattern of strain HRC54 of Herbaspirillum seropedicae. In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16., LATINAMERICAN WORKSHOP OF PGPR, 4., RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Emprapa, 2018. p. 55 Session 5, Short Note V.III| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
| |
| Registros recuperados : 163 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
