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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
08/05/2015 |
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Data da última atualização: |
17/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DANTAS, B. F.; SILVA, R. de C. B. da; RIBEIRO, R. C.; ARAGÃO, C. A. |
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Afiliação: |
BARBARA FRANCA DANTAS, CPATSA; RITA DE CÁSSIA BARBOSA DA SILVA, UFRPE; RENATA CONDURU RIBEIRO; CARLOS ALBERTO ARAGÃO, DTCS/UNEB, Juazeiro-BA. |
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Título: |
Respiration and antioxidant enzymes activity in watermelon seeds and seedlings subjected to salt and temperature stresses. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
American Journal of Experimental Agriculture, v. 7, n. 2, p. 70-77, 2015. |
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ISSN: |
2231-0606 |
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DOI: |
10.9734/AJEA/2015/15749 |
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Idioma: |
Inglês |
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Conteúdo: |
This research aimed to evaluate the effect of salt and temperature stress on water uptake and respiration of watermelon seeds during germination process and to quantify changes in the activity of the antioxidant enzymes ascorbate peroxidase (APX), catalase (CAT) and glutathione-S transferase (GST) involved in protection against reactive oxygen species. The research was performed at the Seed Analysis Laboratory (LASESA) of Embrapa Semi-Arid, Petrolina, Pernambuco State, Brazil, from september to december 2011. The experimental design was completely randomized in a factorial 2x3 (cultivars x stress conditions) for respiration evaluation, 3x4 (cultivars x electrical conductivities) for salt stress assays and 3x3 (cultivars x temperature) for temperature stress assays. The data were submitted to the mean test and evaluated using the standard errors of means. Respiration was measured by CO2 releases by watermelon seeds cv. cv. Crimson Sweet and Charleston Gray evaluated by an infrared gas analyzer, from 0-120 hours of seed imbibition in different environmental conditions (0 dSm-1/25°C, 0 dSm-1/30°C, 4 dSm-1/25°C). The antioxidant enzymes ascorbate peroxidase (APX), catalase (CAT) and glutathione-S transferase (GST) were evaluated in cvs. Crimson Sweet, Charleston Gray and Fairfax seeds and seedlings after five days imbibition in different electrical conductivities (0, 4 and 6 dSm-1) or temperatures (20, 25, 30°C). Crimson Sweet seed respiration rate was increased with increasing temperature, salinity however did not influence the respiration of seeds until the radicle protrusion. The activities of APX and CAT enzymes were antagonistically influenced stresses. The activity of GST was not altered with increased electrical conductivity, however high temperatures led to increase of its activity in watermelon seedlings. The antioxidant detoxification system was activated when imposing temperature and salt stress in all studied watermelon cultivars. Different cultivars of watermelon show different adaptation to salt and temperature stress. MenosThis research aimed to evaluate the effect of salt and temperature stress on water uptake and respiration of watermelon seeds during germination process and to quantify changes in the activity of the antioxidant enzymes ascorbate peroxidase (APX), catalase (CAT) and glutathione-S transferase (GST) involved in protection against reactive oxygen species. The research was performed at the Seed Analysis Laboratory (LASESA) of Embrapa Semi-Arid, Petrolina, Pernambuco State, Brazil, from september to december 2011. The experimental design was completely randomized in a factorial 2x3 (cultivars x stress conditions) for respiration evaluation, 3x4 (cultivars x electrical conductivities) for salt stress assays and 3x3 (cultivars x temperature) for temperature stress assays. The data were submitted to the mean test and evaluated using the standard errors of means. Respiration was measured by CO2 releases by watermelon seeds cv. cv. Crimson Sweet and Charleston Gray evaluated by an infrared gas analyzer, from 0-120 hours of seed imbibition in different environmental conditions (0 dSm-1/25°C, 0 dSm-1/30°C, 4 dSm-1/25°C). The antioxidant enzymes ascorbate peroxidase (APX), catalase (CAT) and glutathione-S transferase (GST) were evaluated in cvs. Crimson Sweet, Charleston Gray and Fairfax seeds and seedlings after five days imbibition in different electrical conductivities (0, 4 and 6 dSm-1) or temperatures (20, 25, 30°C). Crimson Sweet seed respiration rate was increased with increasing ... Mostrar Tudo |
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Palavras-Chave: |
Cucurbitáceas; Watermelon. |
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Thesagro: |
Germinação; Melancia; Semente. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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Marc: |
LEADER 02817naa a2200241 a 4500
001 2015067
005 2016-03-17
008 2015 bl uuuu u00u1 u #d
022 $a2231-0606
024 7 $a10.9734/AJEA/2015/15749$2DOI
100 1 $aDANTAS, B. F.
245 $aRespiration and antioxidant enzymes activity in watermelon seeds and seedlings subjected to salt and temperature stresses.$h[electronic resource]
260 $c2015
520 $aThis research aimed to evaluate the effect of salt and temperature stress on water uptake and respiration of watermelon seeds during germination process and to quantify changes in the activity of the antioxidant enzymes ascorbate peroxidase (APX), catalase (CAT) and glutathione-S transferase (GST) involved in protection against reactive oxygen species. The research was performed at the Seed Analysis Laboratory (LASESA) of Embrapa Semi-Arid, Petrolina, Pernambuco State, Brazil, from september to december 2011. The experimental design was completely randomized in a factorial 2x3 (cultivars x stress conditions) for respiration evaluation, 3x4 (cultivars x electrical conductivities) for salt stress assays and 3x3 (cultivars x temperature) for temperature stress assays. The data were submitted to the mean test and evaluated using the standard errors of means. Respiration was measured by CO2 releases by watermelon seeds cv. cv. Crimson Sweet and Charleston Gray evaluated by an infrared gas analyzer, from 0-120 hours of seed imbibition in different environmental conditions (0 dSm-1/25°C, 0 dSm-1/30°C, 4 dSm-1/25°C). The antioxidant enzymes ascorbate peroxidase (APX), catalase (CAT) and glutathione-S transferase (GST) were evaluated in cvs. Crimson Sweet, Charleston Gray and Fairfax seeds and seedlings after five days imbibition in different electrical conductivities (0, 4 and 6 dSm-1) or temperatures (20, 25, 30°C). Crimson Sweet seed respiration rate was increased with increasing temperature, salinity however did not influence the respiration of seeds until the radicle protrusion. The activities of APX and CAT enzymes were antagonistically influenced stresses. The activity of GST was not altered with increased electrical conductivity, however high temperatures led to increase of its activity in watermelon seedlings. The antioxidant detoxification system was activated when imposing temperature and salt stress in all studied watermelon cultivars. Different cultivars of watermelon show different adaptation to salt and temperature stress.
650 $aGerminação
650 $aMelancia
650 $aSemente
653 $aCucurbitáceas
653 $aWatermelon
700 1 $aSILVA, R. de C. B. da
700 1 $aRIBEIRO, R. C.
700 1 $aARAGÃO, C. A.
773 $tAmerican Journal of Experimental Agriculture$gv. 7, n. 2, p. 70-77, 2015.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Origem |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
05/03/2013 |
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Data da última atualização: |
01/06/2017 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
SOUZA, T. S.; COSTA, J. N.; MARTINEZ, P. M.; LIMA, C. C. V.; ARAÚJO, B. R.; COSTA NETO, A. O.; ANUNCIAÇÃO, A. V. M.; ALMEIDA, M. G. A. R.; PINHEIRO, R. R. |
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Afiliação: |
Universidade Federal da Bahia (UFBA) - Salvador, BA; UFBA - Salvador, BA; Companhia de Desenvolvimento dos Vales do São Francisco e Parnaíba - Juazeiro, BA; Universidade Estadual de Feira de Santana - Feira de Santana, BA.; UFBA - Salvador, BA; UFBA - Salvador, BA; RAYMUNDO RIZALDO PINHEIRO, CNPC. |
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Título: |
Inquérito soro-epidemiológico de Brucella ovis em rebanhos ovinos no semiárido baiano. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Arquivo do Instituto Biológico, São Paulo, v. 79, n. 2, p. 277-281, abr./jun., 2012. |
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Idioma: |
Português |
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Conteúdo: |
Resumo: Com o objetivo de analisar a ocorrência de anticorpos contra Brucella ovis em ovinos no semiárido baiano, este inquérito foi conduzido em rebanhos da microrregião de Juazeiro, Bahia. O teste de imunodifusão em gel de ágar (IDGA) foi utilizado para examinar 694 amostras de soro de 58 rebanhos. Anticorpos para B. ovis foram observados em cinco (0,72%) dos animais investigados. Acredita-se que o baixo número de soropositivos deva-se às características dos sistemas de produção, com predominância do tipo de exploração extensiva de animais localmente adaptados, mestiços e sem raça definida para a produção de carne e pele, com baixa produtividade e tecnificação. [Sero-epidemiological survey for Brucella ovis infection in sheep flocks of Bahia state, Brazil]. Abstract: In order to analyze the occurrence of antibodies to Brucella ovis in sheep in a semi-arid region, this survey was conducted in the micro-region of Juazeiro, Bahia State, Brazil. The agar gel immunodifusion test (AGID) was used to examine 694 serum samples of 58 flocks. Antibodies to B. ovis were found in 5 (0.72%) of the investigated animals. It is believed that this low number of positive cases is related to the characteristics of the production. The predominant system is the extensive one, with presence of locally adapted breeds and crossbred animals, aiming at the production of meat and skin, with low productivity rates and technical development. |
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Palavras-Chave: |
Animal diseaes; Bahia; Brasil; Brucella ovis; Brucelose ovina; IDGA; Ocorrência; Sorologia. |
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Thesagro: |
Doença animal; ELISA; Epidemiologia; Ovino. |
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Thesaurus NAL: |
Brazil; Epidemiology; Sheep. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/77974/1/nt-Inquerito-soroepidemiologico.pdf
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Marc: |
LEADER 02535naa a2200397 a 4500
001 1952227
005 2017-06-01
008 2012 bl uuuu u00u1 u #d
100 1 $aSOUZA, T. S.
245 $aInquérito soro-epidemiológico de Brucella ovis em rebanhos ovinos no semiárido baiano.$h[electronic resource]
260 $c2012
520 $aResumo: Com o objetivo de analisar a ocorrência de anticorpos contra Brucella ovis em ovinos no semiárido baiano, este inquérito foi conduzido em rebanhos da microrregião de Juazeiro, Bahia. O teste de imunodifusão em gel de ágar (IDGA) foi utilizado para examinar 694 amostras de soro de 58 rebanhos. Anticorpos para B. ovis foram observados em cinco (0,72%) dos animais investigados. Acredita-se que o baixo número de soropositivos deva-se às características dos sistemas de produção, com predominância do tipo de exploração extensiva de animais localmente adaptados, mestiços e sem raça definida para a produção de carne e pele, com baixa produtividade e tecnificação. [Sero-epidemiological survey for Brucella ovis infection in sheep flocks of Bahia state, Brazil]. Abstract: In order to analyze the occurrence of antibodies to Brucella ovis in sheep in a semi-arid region, this survey was conducted in the micro-region of Juazeiro, Bahia State, Brazil. The agar gel immunodifusion test (AGID) was used to examine 694 serum samples of 58 flocks. Antibodies to B. ovis were found in 5 (0.72%) of the investigated animals. It is believed that this low number of positive cases is related to the characteristics of the production. The predominant system is the extensive one, with presence of locally adapted breeds and crossbred animals, aiming at the production of meat and skin, with low productivity rates and technical development.
650 $aBrazil
650 $aEpidemiology
650 $aSheep
650 $aDoença animal
650 $aELISA
650 $aEpidemiologia
650 $aOvino
653 $aAnimal diseaes
653 $aBahia
653 $aBrasil
653 $aBrucella ovis
653 $aBrucelose ovina
653 $aIDGA
653 $aOcorrência
653 $aSorologia
700 1 $aCOSTA, J. N.
700 1 $aMARTINEZ, P. M.
700 1 $aLIMA, C. C. V.
700 1 $aARAÚJO, B. R.
700 1 $aCOSTA NETO, A. O.
700 1 $aANUNCIAÇÃO, A. V. M.
700 1 $aALMEIDA, M. G. A. R.
700 1 $aPINHEIRO, R. R.
773 $tArquivo do Instituto Biológico, São Paulo$gv. 79, n. 2, p. 277-281, abr./jun., 2012.
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Embrapa Caprinos e Ovinos (CNPC) |
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