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Registro Completo |
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Biblioteca(s): |
Embrapa Agrossilvipastoril; Embrapa Meio Ambiente. |
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Data corrente: |
20/02/2017 |
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Data da última atualização: |
02/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROMÃO-DUMARESQ, A. S.; DOURADO, M. N.; FAVARO, L. C. de L.; MENDES, R.; FERREIRA, A.; ARAÚJO, W. L. |
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Afiliação: |
ALINE SILVA ROMÃO-DUMARESQ; MANUELLA NÓBREGA DOURADO; LEIA CECILIA DE LIMA FAVARO, CNPAE; RODRIGO MENDES, CNPMA; ANDERSON FERREIRA, ESALQ-USP; WELINGTON LUIZ ARAÚJO, ICB-USP. |
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Título: |
Diversity of cultivated fungi associated with conventional and transgenic sugarcane and the interaction between endophytic trichoderma virens and host plant. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Plos One, v. 11, n. 7, p. e0158974, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Plant-associated fungi are considered a vast source for biotechnological processes whose potential has been poorly explored. The interactions and diversity of sugarcane, one of the most important crops in Brazil, have been rarely studied, mainly concerning fungal communities and their interactions with transgenic plants. Taking this into consideration, the purpose of this study was, based on culture dependent strategy, to determine the structure and diversity of the fungal community (root endophytes and rhizosphere) associated with two varieties of sugarcane, a non-genetically modified (SP80-1842) variety and its genetically modified counterpart (IMI-1, expressing imazapyr herbicide resistance). For this, the sugarcane varieties were evaluated in three sampling times (3, 10 and 17 months after planting) under two crop management (weeding and herbicide treatments). In addition, a strain of Trichoderma virens, an endophyte isolated from sugarcane with great potential as a biological control, growth promotion and enzyme production agent, was selected for the fungal-plant interaction assays. The results of the isolation, characterization and evaluation of fungal community changes showed that the sugarcane fungal community is composed of at least 35 different genera, mostly in the phylum Ascomycota. Many genera are observed at very low frequencies among a few most abundant genera, some of which were isolated from specific plant sites (e.g., the roots or the rhizosphere). An assessment of the possible effects upon the fungal community showed that the plant growth stage was the only factor that significantly affected the community?s structure. Moreover, if transgenic effects are present, they may be minor compared to other natural sources of variation. The results of interaction studies using the Green fluorescent protein (GFP)-expressing T. virens strain T.v.223 revealed that this fungus did not promote any phenotypic changes in the host plant and was found mostly in the roots where it formed a dense mycelial cover and was able to penetrate the intercellular spaces of the root epidermis upper layers. The ability of T. virens to colonize plant roots suggests a potential for protecting plant health, inhibiting pathogens or inducing systemic resistance. MenosAbstract: Plant-associated fungi are considered a vast source for biotechnological processes whose potential has been poorly explored. The interactions and diversity of sugarcane, one of the most important crops in Brazil, have been rarely studied, mainly concerning fungal communities and their interactions with transgenic plants. Taking this into consideration, the purpose of this study was, based on culture dependent strategy, to determine the structure and diversity of the fungal community (root endophytes and rhizosphere) associated with two varieties of sugarcane, a non-genetically modified (SP80-1842) variety and its genetically modified counterpart (IMI-1, expressing imazapyr herbicide resistance). For this, the sugarcane varieties were evaluated in three sampling times (3, 10 and 17 months after planting) under two crop management (weeding and herbicide treatments). In addition, a strain of Trichoderma virens, an endophyte isolated from sugarcane with great potential as a biological control, growth promotion and enzyme production agent, was selected for the fungal-plant interaction assays. The results of the isolation, characterization and evaluation of fungal community changes showed that the sugarcane fungal community is composed of at least 35 different genera, mostly in the phylum Ascomycota. Many genera are observed at very low frequencies among a few most abundant genera, some of which were isolated from specific plant sites (e.g., the roots or the rhizosphere)... Mostrar Tudo |
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Palavras-Chave: |
Cana-de-açúcar convencional; Cana-de-açúcar transgênica; Diversidade de fungos cultivados; Trichoderma virens endofítico. |
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Thesagro: |
Planta Hospedeira. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03124naa a2200241 a 4500
001 2064860
005 2017-03-02
008 2016 bl --- 0-- u #d
100 1 $aROMÃO-DUMARESQ, A. S.
245 $aDiversity of cultivated fungi associated with conventional and transgenic sugarcane and the interaction between endophytic trichoderma virens and host plant.$h[electronic resource]
260 $c2016
520 $aAbstract: Plant-associated fungi are considered a vast source for biotechnological processes whose potential has been poorly explored. The interactions and diversity of sugarcane, one of the most important crops in Brazil, have been rarely studied, mainly concerning fungal communities and their interactions with transgenic plants. Taking this into consideration, the purpose of this study was, based on culture dependent strategy, to determine the structure and diversity of the fungal community (root endophytes and rhizosphere) associated with two varieties of sugarcane, a non-genetically modified (SP80-1842) variety and its genetically modified counterpart (IMI-1, expressing imazapyr herbicide resistance). For this, the sugarcane varieties were evaluated in three sampling times (3, 10 and 17 months after planting) under two crop management (weeding and herbicide treatments). In addition, a strain of Trichoderma virens, an endophyte isolated from sugarcane with great potential as a biological control, growth promotion and enzyme production agent, was selected for the fungal-plant interaction assays. The results of the isolation, characterization and evaluation of fungal community changes showed that the sugarcane fungal community is composed of at least 35 different genera, mostly in the phylum Ascomycota. Many genera are observed at very low frequencies among a few most abundant genera, some of which were isolated from specific plant sites (e.g., the roots or the rhizosphere). An assessment of the possible effects upon the fungal community showed that the plant growth stage was the only factor that significantly affected the community?s structure. Moreover, if transgenic effects are present, they may be minor compared to other natural sources of variation. The results of interaction studies using the Green fluorescent protein (GFP)-expressing T. virens strain T.v.223 revealed that this fungus did not promote any phenotypic changes in the host plant and was found mostly in the roots where it formed a dense mycelial cover and was able to penetrate the intercellular spaces of the root epidermis upper layers. The ability of T. virens to colonize plant roots suggests a potential for protecting plant health, inhibiting pathogens or inducing systemic resistance.
650 $aPlanta Hospedeira
653 $aCana-de-açúcar convencional
653 $aCana-de-açúcar transgênica
653 $aDiversidade de fungos cultivados
653 $aTrichoderma virens endofítico
700 1 $aDOURADO, M. N.
700 1 $aFAVARO, L. C. de L.
700 1 $aMENDES, R.
700 1 $aFERREIRA, A.
700 1 $aARAÚJO, W. L.
773 $tPlos One$gv. 11, n. 7, p. e0158974, 2016.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Biblioteca |
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| Registros recuperados : 56 | |
| 4. |  | LACAVA, P. T.; ANDREOTE, F. D.; ARAÚJO, W. L.; AZEVEDO, J. L. Caracterização da comunidade bacteriana endofítica de citros por isolamento, PCR específico e DGGE. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 41, n. 4, p. 637-642, abr. 2006 Título em inglês: Characterization of the endophytic bacterial community from citrus by isolation, specific PCR and DGGE.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 6. | | FAVARO, L. C. de L.; SEBASTIANES, F. L. DE S.; ARAUJO, W. L. Epicoccum nigrumP16, a Sugarcane Endophyte, Produces Antifungal Compounds and Induces Root Growth. PLoS ONE, v. 7, n. 6, e36826, p. 1-10, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 12. | | TEIXEIRA, M. A.; MELO, I. S. de; ARAUJO, W. L.; PROCOPIO, R. E. L. Isolamento e caracterização de bactérias endofíticas em mandioca (Manihot esculenta Crantz). In: SIMPÓSIO, 4., REUNIÃO DE AVALIAÇÃO DO PROGRAMA BIOTA/FAPESP, 4., Águas de Lindóia, 2003. Anais... Águas de Lindóia: FAPESP, 2003.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 15. | | PROCOPIO, A. R. L.; ARAÚJO, W. L.; MELO, I. S. de. Diversidade da comunidade bacteriana da rizosfera de arroz e mata sob influência do herbicida propanil, utilizando DGGE. In: ENCONTRO NACIONAL DE MICROBIOLOGIA AMBIENTAL, 10., 2006, Goiânia, GO. [Anais...]. Goiânia, GO: UFG, 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 16. | | CARAVIERI, F. A.; FERREIRA, A. J.; FERREIRA, A.; CLIVATI, D.; MIRANDA, V. F. O. de; ARAÚJO, W. L. Bacterial community associated with traps of the carnivorous plants Utricularia hydrocarpa and Genlisea filiformis Aquatic Botany, Amsterdam, v. 116, p. 8-12, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 17. | | DIAS, A. C. F.; ANDREOTE, F. D.; RIGONATO, J.; FIORE, M. F.; MELO, I. S. de; ARAUJO, W. L. The bacterial diversity in a Brazilian non-disturbed mangrove sediment. Antonie van Leeuwenhoek, v. 98, n. 4, p. 541-551, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 19. | | TORRES, A. R.; ARAÚJO, W. L.; CURSINO, L.; ROSSETTO, P. de B.; MONDIN, M.; HUNGRIA, M.; AZEVEDO, J. L. Colonization of Madagascar periwinkle (Catharanthus roseus), by endophytes encoding gfp marker. Archives of Microbiology, Berlin, v. 195, n. 7, p. 483-489, Jul. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 20. | | ROMÃO-DUMARESQ, A. S.; DOURADO, M. N.; FAVARO, L. C. de L.; MENDES, R.; FERREIRA, A.; ARAÚJO, W. L. Diversity of cultivated fungi associated with conventional and transgenic sugarcane and the interaction between endophytic trichoderma virens and host plant. Plos One, v. 11, n. 7, p. e0158974, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 56 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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