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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Caprinos e Ovinos. |
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Data corrente: |
27/09/2019 |
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Data da última atualização: |
07/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AZEVEDO, D. A. A. de; MONTEIRO, J. P.; PINHEIRO, R. R.; MUDADU, M. de A.; ANDRIOLI, A.; ARAÚJO, J. F.; SOUSA, A. L. M. de; SIDER, L. H.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S. |
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Afiliação: |
DALVA ALANA ARAGÃO DE AZEVEDO, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil; JOMAR PATRICIO MONTEIRO, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; MAURICIO DE ALVARENGA MUDADU, CNPTIA; ALICE ANDRIOLI, CNPC; JUSCILÂNIA FURTADO ARAÚJO, Northeast Biotechnology Network, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil; ANA LÍDIA MADEIRA DE SOUSA, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil; LUCIA HELENA SIDER, CNPC; RENATO MESQUITA PEIXOTO, Universidade Federal do Acre (UFAC) - Rio Branco, AC, Brazil; MARIA FÁTIMA DA SILVA TEIXEIRA, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil. |
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Título: |
Molecular characterization of circulating strains of small ruminant lentiviruses in Brazil based on complete gag and pol genes. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Small Ruminant Research, v. 177, p. 160-166, 2019. |
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DOI: |
https://doi.org/10.1016/j.smallrumres.2019.06.011 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Small ruminant lentiviruses (SRLV) are widely distributed in the world and cause productive and economic losses. These pathogens have high genetic variability, which hinders diagnosis by molecular methods. Molecular characterization studies are good strategies for elaborating programs aimed to control or eradicate diseases. Hence, this study aimed to sequence, characterize and compare strains of SRLV, which were isolated from naturally infected goats from the states of Ceará, Rio Grande do Norte and Minas Gerais, Brazil, based on total nucleotide sequences of gag and pol genes. SRLV isolates from the microbiological collection of Sheep and Goat of Embrapa were cultured in goat synovial membrane. Supernatants containing cells were stored and viral particles were purified with sucrose density gradient centrifugation for RNA extraction. Then, sequencing in HiSeq 2500 platform (Illumina) was performed to obtain the total genome of the SRLV strains. The software MEGA version 7.0 was used for sequence editing and phylogeny analysis. A total of 17 isolates were multiplied in cellular culture and six were viable, which were two from Minas Gerais (MG), one from Rio Grande do Norte (RN) and three from Ceará (CE). Two sequences were obtained from the sequencing by synthesis, one from MG (BRMG CNPC) and one from RN (BRRN CNPC). From the total SRLV genome (9,200 bases), sample BRMG CNPC generated a consensus of 6,378 bases, approximately 69,34% of the viral genome and sample BRRN CNPC generated a consensus of 5,351 bases, which comprised 58.16% of the total genome. Samples in this study demonstrated closer phylogenetic relation with genotype B (B1 group). The remaining strains sequenced in Brazil are also present in the same group with the exception of three samples from genotype A. Immunodominant regions of gag gene and motifs of pol gene were similar to the standard CAEV Cork strain. For the first time, the complete gag and pol genes were sequenced in Brazil from the strain BRMG CNPC isolated in Minas Gerais state. In addition, 2490 bases from the total of 3316 bases were sequenced from the pol gene of the BRRN CNPC sample from Rio Grande do Norte. MenosAbstract: Small ruminant lentiviruses (SRLV) are widely distributed in the world and cause productive and economic losses. These pathogens have high genetic variability, which hinders diagnosis by molecular methods. Molecular characterization studies are good strategies for elaborating programs aimed to control or eradicate diseases. Hence, this study aimed to sequence, characterize and compare strains of SRLV, which were isolated from naturally infected goats from the states of Ceará, Rio Grande do Norte and Minas Gerais, Brazil, based on total nucleotide sequences of gag and pol genes. SRLV isolates from the microbiological collection of Sheep and Goat of Embrapa were cultured in goat synovial membrane. Supernatants containing cells were stored and viral particles were purified with sucrose density gradient centrifugation for RNA extraction. Then, sequencing in HiSeq 2500 platform (Illumina) was performed to obtain the total genome of the SRLV strains. The software MEGA version 7.0 was used for sequence editing and phylogeny analysis. A total of 17 isolates were multiplied in cellular culture and six were viable, which were two from Minas Gerais (MG), one from Rio Grande do Norte (RN) and three from Ceará (CE). Two sequences were obtained from the sequencing by synthesis, one from MG (BRMG CNPC) and one from RN (BRRN CNPC). From the total SRLV genome (9,200 bases), sample BRMG CNPC generated a consensus of 6,378 bases, approximately 69,34% of the viral genome and sample BR... Mostrar Tudo |
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Palavras-Chave: |
Caracterização molecular; Molecular characterization; SRLV. |
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Thesaurus Nal: |
Genome; Goats; Lentivirus; Phylogeny; Sheep. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 03206naa a2200337 a 4500
001 2112529
005 2019-11-07
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.smallrumres.2019.06.011$2DOI
100 1 $aAZEVEDO, D. A. A. de
245 $aMolecular characterization of circulating strains of small ruminant lentiviruses in Brazil based on complete gag and pol genes.$h[electronic resource]
260 $c2019
520 $aAbstract: Small ruminant lentiviruses (SRLV) are widely distributed in the world and cause productive and economic losses. These pathogens have high genetic variability, which hinders diagnosis by molecular methods. Molecular characterization studies are good strategies for elaborating programs aimed to control or eradicate diseases. Hence, this study aimed to sequence, characterize and compare strains of SRLV, which were isolated from naturally infected goats from the states of Ceará, Rio Grande do Norte and Minas Gerais, Brazil, based on total nucleotide sequences of gag and pol genes. SRLV isolates from the microbiological collection of Sheep and Goat of Embrapa were cultured in goat synovial membrane. Supernatants containing cells were stored and viral particles were purified with sucrose density gradient centrifugation for RNA extraction. Then, sequencing in HiSeq 2500 platform (Illumina) was performed to obtain the total genome of the SRLV strains. The software MEGA version 7.0 was used for sequence editing and phylogeny analysis. A total of 17 isolates were multiplied in cellular culture and six were viable, which were two from Minas Gerais (MG), one from Rio Grande do Norte (RN) and three from Ceará (CE). Two sequences were obtained from the sequencing by synthesis, one from MG (BRMG CNPC) and one from RN (BRRN CNPC). From the total SRLV genome (9,200 bases), sample BRMG CNPC generated a consensus of 6,378 bases, approximately 69,34% of the viral genome and sample BRRN CNPC generated a consensus of 5,351 bases, which comprised 58.16% of the total genome. Samples in this study demonstrated closer phylogenetic relation with genotype B (B1 group). The remaining strains sequenced in Brazil are also present in the same group with the exception of three samples from genotype A. Immunodominant regions of gag gene and motifs of pol gene were similar to the standard CAEV Cork strain. For the first time, the complete gag and pol genes were sequenced in Brazil from the strain BRMG CNPC isolated in Minas Gerais state. In addition, 2490 bases from the total of 3316 bases were sequenced from the pol gene of the BRRN CNPC sample from Rio Grande do Norte.
650 $aGenome
650 $aGoats
650 $aLentivirus
650 $aPhylogeny
650 $aSheep
653 $aCaracterização molecular
653 $aMolecular characterization
653 $aSRLV
700 1 $aMONTEIRO, J. P.
700 1 $aPINHEIRO, R. R.
700 1 $aMUDADU, M. de A.
700 1 $aANDRIOLI, A.
700 1 $aARAÚJO, J. F.
700 1 $aSOUSA, A. L. M. de
700 1 $aSIDER, L. H.
700 1 $aPEIXOTO, R. M.
700 1 $aTEIXEIRA, M. F. da S.
773 $tSmall Ruminant Research$gv. 177, p. 160-166, 2019.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca |
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Tipo/Formato |
Classificação |
Cutter |
Registro |
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URL |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
20/03/2009 |
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Data da última atualização: |
26/03/2009 |
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Tipo da produção científica: |
Monitoramento/Zoneamento |
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Autoria: |
OLIVEIRA NETO, M. B. de; LEITE, A. P.; SANTOS, J. C. P. dos; PARAHYBA, R. da B. V.; SILVA, M. S. L. da; LUCENA, A. M. A. de. |
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Afiliação: |
Manoel Batista de Oliveira neto, Embrapa Solos UEP; Aldo Pereira Leite, Embrapa Solos UEP; José Carlos Pereira dos Santos, Embrapa Solos UEP; Roberto da Boa Viagem Parahyba, Embrapa Solos UEP; Maria Sonia Lopes da Silva, Embrapa Solos UEP; Andréa Maria Alves de Lucena, Embrapa Solos UEP. |
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Título: |
Mapa de solos do município de Acauã, Piauí. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Recife: Embrapa Solos, 2008. |
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Páginas: |
1 mapa. |
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Descrição Física: |
mapa color.; escala 1:100.000. |
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Idioma: |
Português |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00500nem a2200169 a 4500
001 1340028
005 2009-03-26
008 2008 bl uuuu u0uu1 u #d
100 1 $aOLIVEIRA NETO, M. B. de
245 $aMapa de solos do município de Acauã, Piauí.
260 $aRecife: Embrapa Solos$c2008
300 $a1 mapa.$cmapa color.; escala 1:100.000.
700 1 $aLEITE, A. P.
700 1 $aSANTOS, J. C. P. dos
700 1 $aPARAHYBA, R. da B. V.
700 1 $aSILVA, M. S. L. da
700 1 $aLUCENA, A. M. A. de
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Embrapa Solos (CNPS) |
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