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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
01/10/1998 |
Data da última atualização: |
01/10/1998 |
Autoria: |
SOARES FILHO, W. dos S.; PELACANI, C. R.; SOUZA, A. da S.; CUNHA SOBRINHO, A. P. da; ARAUJO, E. F. de. |
Afiliação: |
EMBRAPA-CNPMF. |
Título: |
Influencia dos tegumentos externo e interno na germinacao de sementes de citros: implicacoes na sobrevivencia de "seedlings" hibridos |
Ano de publicação: |
1994 |
Fonte/Imprenta: |
Cruz das Almas: EMBRAPA-CNPMF, 1994 |
Volume: |
v.2 |
Páginas: |
p.475-476 |
Idioma: |
Português |
Notas: |
In: CONGRESSO BRASILEIRO DE FRUTICULTURA. Resumos... Salvador: SBF, 1994. |
Conteúdo: |
O presente trabalho teve como objetivo verificar a possibilidade de promover a germinacao de sementes de citros sem a necessidade de realizar o cultivo in vitro de seus embrioes de modo a garantir a sobrevivencia de eventuais "seedlings" hibridos, obtidos a partir de cruzamentos controlados. Foi conduzido no Centro Nacional de Pesquisa de Mandioca e Fruticultura Tropical - CNPMF, pertencente a Empresa Brasileira de Pesquisa Agropecuaria - EMBRAPA, localizado no municipio de Cruz das Almas, Reconcavo Baiano, durante o periodo de janeiro a maio de 1994. As variedades estudadas compreenderam: tangerina `Clementina' selecao Palazelli (Citrus clementina), tangerina-tangelo `Lee' [Clementina' x tangelo `Orlando' (pomelo `Duncan' C. paradisi x tangerina `Dancy' C. tangerina)], tangerina `Sunki' (C. sunki), limao `Cravo' selecao Santa Barbara (C. limonia) e tangor `Dweet' [laranja `Mediterranea' (C. sinensis) x tangerina `Dancy']. As duas primeiras sao monoembrionicas, a terceira possui baixo grau de poliembrionia e as ultimas apresentam grau moderado de poliembrionia. |
Palavras-Chave: |
Melhoramento genetico; Tangerina Clementina; Tangerina Sunki; Tangerina-tangelo Lee; Tangor Dweet. |
Thesagro: |
Hibridação; Limão Cravo. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01989naa a2200289 a 4500 001 1645690 005 1998-10-01 008 1994 bl uuuu u00u1 u #d 100 1 $aSOARES FILHO, W. dos S. 245 $aInfluencia dos tegumentos externo e interno na germinacao de sementes de citros$bimplicacoes na sobrevivencia de "seedlings" hibridos 260 $c1994 300 $ap.475-476 v.2 490 $vv.2 500 $aIn: CONGRESSO BRASILEIRO DE FRUTICULTURA. Resumos... Salvador: SBF, 1994. 520 $aO presente trabalho teve como objetivo verificar a possibilidade de promover a germinacao de sementes de citros sem a necessidade de realizar o cultivo in vitro de seus embrioes de modo a garantir a sobrevivencia de eventuais "seedlings" hibridos, obtidos a partir de cruzamentos controlados. Foi conduzido no Centro Nacional de Pesquisa de Mandioca e Fruticultura Tropical - CNPMF, pertencente a Empresa Brasileira de Pesquisa Agropecuaria - EMBRAPA, localizado no municipio de Cruz das Almas, Reconcavo Baiano, durante o periodo de janeiro a maio de 1994. As variedades estudadas compreenderam: tangerina `Clementina' selecao Palazelli (Citrus clementina), tangerina-tangelo `Lee' [Clementina' x tangelo `Orlando' (pomelo `Duncan' C. paradisi x tangerina `Dancy' C. tangerina)], tangerina `Sunki' (C. sunki), limao `Cravo' selecao Santa Barbara (C. limonia) e tangor `Dweet' [laranja `Mediterranea' (C. sinensis) x tangerina `Dancy']. As duas primeiras sao monoembrionicas, a terceira possui baixo grau de poliembrionia e as ultimas apresentam grau moderado de poliembrionia. 650 $aHibridação 650 $aLimão Cravo 653 $aMelhoramento genetico 653 $aTangerina Clementina 653 $aTangerina Sunki 653 $aTangerina-tangelo Lee 653 $aTangor Dweet 700 1 $aPELACANI, C. R. 700 1 $aSOUZA, A. da S. 700 1 $aCUNHA SOBRINHO, A. P. da 700 1 $aARAUJO, E. F. de 773 $tCruz das Almas: EMBRAPA-CNPMF, 1994
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
23/08/2017 |
Data da última atualização: |
23/09/2019 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA FABJAN, J. M. G. de; ALFRADIQUE, V. A. P.; CORTÊS, L. R.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. |
Afiliação: |
Gláucia Mota Bragança, Universidade Federal Fluminense (UFF) - Rio de Janeiro, RJ; Ribrio Ivan Tavares Pereira Batista; Joanna Maria Gonçalves de Souza Fabjan; Vivian Angélico Pereira Alfradique; Luana Rangel Côrtes; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Zandonadi Brandao. |
Título: |
FSH dose and strategy of administration during ovarian stimulation alter the gene expression profile in ovine cumulus-oocyte complexes. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Animal Reprodroduction, v. 14, n. 3, p. 746, Jul./Sept. 2017. |
Idioma: |
Inglês |
Notas: |
Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Cabo de Santo Agostinho, PE, Brazil, August 17 to 19, 2017. |
Conteúdo: |
Ovarian stimulation is an important tool to increase the number of oocytes obtained by laparoscopy for the in vitro production of embryos (IVP). In sheep, different concentrations of FSH administered in single dose (SD) or multiple doses (MD) have been adopted. In parallel, the oocyte quality is fundamental for IVP success, so strategies to produce more competent oocytes have been evaluated. The aim of this study was to evaluate the gene expression profile of BCB+ COC from different hormonal protocols of ovarian stimulation in Santa Inês ewes. To achieve that, a cross-over design was used, where 12 pluriparous ewes had their follicular wave synchronized (Balaro et al., Domest Anim Endocrinol, 54: 10-14, 2016). At 80 h after progestogen implant removal, all ewes received a new vaginal sponge and it started the stimulation by administration of: 80 (Group 1 - 80-SD) or 120 (Group 2 - 120-SD) mg FSH (Folltropin-V®, Bioniche Animal Health, Ontario, Canada) and 300 IU of eCG both in single dose, or 80 (Group 3 - 80-MD) or 120 (Group 4 - 120-MD) in decreasing doses (50/30/20%) every 12 h. The COCs were recovered by laparoscopy and classified morphologically in grade I / II (homogeneous ooplasm and more than 3 cumulus cells layers), III (homogeneous ooplasm and less than 3 cumulus cells layers or partially denuded) and IV (heterogeneous ooplasm or degenerate). For inference of the development competence GI, II and III COCs were stained with bright cresyl blue (BCB) and classified into: BCB+ (competent) and BCB- (non-competent). These variables were compared by ANOVA followed by Tukey test. The abundance of mRNA that encodes proteins associated with steroidogenesis (STAR, FSHr, LHr and ER?), oocyte quality (MATER, BMP15, GDF9 and ZAR1) and apoptosis (BAX and Bcl-2) was assessed by real-time qPCR normalized with GAPDH in BCB+ COCs. The abundance of gene transcripts associated with steroidogenesis was down-regulated (P <0.05) with increasing FSH concentration, when administration was performed in a single dose (80-SD and 120-SD). On the other hand, when the administration was performed in MD, only the LHr was down-regulated (80-MD and 120-MD). In the 80-MD group, FSHr and Er? were down-regulated (P <0.05) in comparison with 80-SD. For genes related with oocyte quality, 80-MD showed up-regulation (P <0.05) to MATER (when compared to 80-SD), ZAR1 and MATER (compared to 120-SD). Nonetheless, apoptosis genes were not affected. These data demonstrate that the FSH dose and strategy of administration affect the gene expression profile in ovine COCs. Subsequent studies are necessary to assess the effect of this change on maturation rate and developmental competence. MenosOvarian stimulation is an important tool to increase the number of oocytes obtained by laparoscopy for the in vitro production of embryos (IVP). In sheep, different concentrations of FSH administered in single dose (SD) or multiple doses (MD) have been adopted. In parallel, the oocyte quality is fundamental for IVP success, so strategies to produce more competent oocytes have been evaluated. The aim of this study was to evaluate the gene expression profile of BCB+ COC from different hormonal protocols of ovarian stimulation in Santa Inês ewes. To achieve that, a cross-over design was used, where 12 pluriparous ewes had their follicular wave synchronized (Balaro et al., Domest Anim Endocrinol, 54: 10-14, 2016). At 80 h after progestogen implant removal, all ewes received a new vaginal sponge and it started the stimulation by administration of: 80 (Group 1 - 80-SD) or 120 (Group 2 - 120-SD) mg FSH (Folltropin-V®, Bioniche Animal Health, Ontario, Canada) and 300 IU of eCG both in single dose, or 80 (Group 3 - 80-MD) or 120 (Group 4 - 120-MD) in decreasing doses (50/30/20%) every 12 h. The COCs were recovered by laparoscopy and classified morphologically in grade I / II (homogeneous ooplasm and more than 3 cumulus cells layers), III (homogeneous ooplasm and less than 3 cumulus cells layers or partially denuded) and IV (heterogeneous ooplasm or degenerate). For inference of the development competence GI, II and III COCs were stained with bright cresyl blue (BCB) and classified in... Mostrar Tudo |
Palavras-Chave: |
Embryo Technology; Expressão gênica; FSH; In vitro embryo production; Produção de embriões in vitro. |
Thesagro: |
Ovino; Reprodução animal; Transferência de embrião. |
Thesaurus NAL: |
Gene expression; Ova; Reproduction; Sheep. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162972/1/cnpc-2017-FSH.pdf
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Marc: |
LEADER 03935nam a2200349 a 4500 001 2074359 005 2019-09-23 008 2017 bl uuuu u00u1 u #d 100 1 $aBRAGANÇA, G. M. 245 $aFSH dose and strategy of administration during ovarian stimulation alter the gene expression profile in ovine cumulus-oocyte complexes.$h[electronic resource] 260 $aAnimal Reprodroduction, v. 14, n. 3, p. 746, Jul./Sept. 2017.$c2017 500 $aProceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Cabo de Santo Agostinho, PE, Brazil, August 17 to 19, 2017. 520 $aOvarian stimulation is an important tool to increase the number of oocytes obtained by laparoscopy for the in vitro production of embryos (IVP). In sheep, different concentrations of FSH administered in single dose (SD) or multiple doses (MD) have been adopted. In parallel, the oocyte quality is fundamental for IVP success, so strategies to produce more competent oocytes have been evaluated. The aim of this study was to evaluate the gene expression profile of BCB+ COC from different hormonal protocols of ovarian stimulation in Santa Inês ewes. To achieve that, a cross-over design was used, where 12 pluriparous ewes had their follicular wave synchronized (Balaro et al., Domest Anim Endocrinol, 54: 10-14, 2016). At 80 h after progestogen implant removal, all ewes received a new vaginal sponge and it started the stimulation by administration of: 80 (Group 1 - 80-SD) or 120 (Group 2 - 120-SD) mg FSH (Folltropin-V®, Bioniche Animal Health, Ontario, Canada) and 300 IU of eCG both in single dose, or 80 (Group 3 - 80-MD) or 120 (Group 4 - 120-MD) in decreasing doses (50/30/20%) every 12 h. The COCs were recovered by laparoscopy and classified morphologically in grade I / II (homogeneous ooplasm and more than 3 cumulus cells layers), III (homogeneous ooplasm and less than 3 cumulus cells layers or partially denuded) and IV (heterogeneous ooplasm or degenerate). For inference of the development competence GI, II and III COCs were stained with bright cresyl blue (BCB) and classified into: BCB+ (competent) and BCB- (non-competent). These variables were compared by ANOVA followed by Tukey test. The abundance of mRNA that encodes proteins associated with steroidogenesis (STAR, FSHr, LHr and ER?), oocyte quality (MATER, BMP15, GDF9 and ZAR1) and apoptosis (BAX and Bcl-2) was assessed by real-time qPCR normalized with GAPDH in BCB+ COCs. The abundance of gene transcripts associated with steroidogenesis was down-regulated (P <0.05) with increasing FSH concentration, when administration was performed in a single dose (80-SD and 120-SD). On the other hand, when the administration was performed in MD, only the LHr was down-regulated (80-MD and 120-MD). In the 80-MD group, FSHr and Er? were down-regulated (P <0.05) in comparison with 80-SD. For genes related with oocyte quality, 80-MD showed up-regulation (P <0.05) to MATER (when compared to 80-SD), ZAR1 and MATER (compared to 120-SD). Nonetheless, apoptosis genes were not affected. These data demonstrate that the FSH dose and strategy of administration affect the gene expression profile in ovine COCs. Subsequent studies are necessary to assess the effect of this change on maturation rate and developmental competence. 650 $aGene expression 650 $aOva 650 $aReproduction 650 $aSheep 650 $aOvino 650 $aReprodução animal 650 $aTransferência de embrião 653 $aEmbryo Technology 653 $aExpressão gênica 653 $aFSH 653 $aIn vitro embryo production 653 $aProdução de embriões in vitro 700 1 $aBATISTA, R. I. T. P. 700 1 $aSOUZA FABJAN, J. M. G. de 700 1 $aALFRADIQUE, V. A. P. 700 1 $aCORTÊS, L. R. 700 1 $aCAMARGO, L. S. de A. 700 1 $aFONSECA, J. F. da 700 1 $aBRANDÃO, F. Z.
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