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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
20/12/2004 |
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Data da última atualização: |
30/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOMES, E. A.; KASUYA, M. C. M.; BARROS, E. G. de; BORGES, A. C.; ARAUJO, E. F. |
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Afiliação: |
ELIANE APARECIDA GOMES, CNPMS. |
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Título: |
Polymorphism in the internal transcribed spacer (ITS) of the ribosomal DNA of 26 isolates of ectomycorrhizal fungi. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 25, n. 4, p. 477-483, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
Inter- and intraspecific variation among 26 isolates of ectomycorrhizal fungi belonging to 8 genera and 19 species were evaluated by analysis of the internal transcribed sequence (ITS) of the rDNA region using restriction fragment length polymorphism (RFLP). The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and then cleaved with different restriction enzymes. Amplification products, which ranged between 560 and 750 base pairs (bp), were obtained for all the isolates analyzed. The degree of polymorphism observed did not allow proper identification of most of the isolates. Cleavage of amplified fragments with the restriction enzymes Alu I, Hae III, Hinf I, and Hpa II revealed extensive polymorphism. All eight genera and most species presented specific restriction patterns. Species not identifiable by a specific pattern belonged to two genera: Rhizopogon (R. nigrescens, R. reaii, R. roseolus, R. rubescens and Rhizopogon sp.), and Laccaria (L. bicolor and L. amethystea). Our data confirm the potential of ITS region PCR-RFLP for the molecular characterization of ectomycorrhizal fungi and their identification and monitoring in artificial inoculation programs. |
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Palavras-Chave: |
Ectomycorrhizal fungi; Internal transcribed spacer (ITS); PCR-RFLP; Ribosomal DNA (rDNA). |
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Thesaurus Nal: |
DNA fingerprinting. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32594/1/Polymorphism-internal.pdf
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Marc: |
LEADER 01964naa a2200229 a 4500
001 1488489
005 2018-05-30
008 2002 bl uuuu u00u1 u #d
100 1 $aGOMES, E. A.
245 $aPolymorphism in the internal transcribed spacer (ITS) of the ribosomal DNA of 26 isolates of ectomycorrhizal fungi.$h[electronic resource]
260 $c2002
520 $aInter- and intraspecific variation among 26 isolates of ectomycorrhizal fungi belonging to 8 genera and 19 species were evaluated by analysis of the internal transcribed sequence (ITS) of the rDNA region using restriction fragment length polymorphism (RFLP). The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and then cleaved with different restriction enzymes. Amplification products, which ranged between 560 and 750 base pairs (bp), were obtained for all the isolates analyzed. The degree of polymorphism observed did not allow proper identification of most of the isolates. Cleavage of amplified fragments with the restriction enzymes Alu I, Hae III, Hinf I, and Hpa II revealed extensive polymorphism. All eight genera and most species presented specific restriction patterns. Species not identifiable by a specific pattern belonged to two genera: Rhizopogon (R. nigrescens, R. reaii, R. roseolus, R. rubescens and Rhizopogon sp.), and Laccaria (L. bicolor and L. amethystea). Our data confirm the potential of ITS region PCR-RFLP for the molecular characterization of ectomycorrhizal fungi and their identification and monitoring in artificial inoculation programs.
650 $aDNA fingerprinting
653 $aEctomycorrhizal fungi
653 $aInternal transcribed spacer (ITS)
653 $aPCR-RFLP
653 $aRibosomal DNA (rDNA)
700 1 $aKASUYA, M. C. M.
700 1 $aBARROS, E. G. de
700 1 $aBORGES, A. C.
700 1 $aARAUJO, E. F.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 25, n. 4, p. 477-483, 2002.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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