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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
23/03/2022 |
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Data da última atualização: |
24/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DELABONA, P. S.; CODIMA, C. A.; RAMONI, J.; ZUBIETA, M. P.; ARAÚJO, B. M. de; FARINAS, C. S.; PRADELLA, J. G. C.; SEIBOTH, B. |
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Afiliação: |
CRISTIANE SANCHEZ FARINAS, CNPDIA. |
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Título: |
The impact of putative methyltransferase overexpression on the Trichoderma harzianum cellulolytic system for biomass conversion. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Bioresource Technology, v. 313, 12361, 2020. |
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Páginas: |
1 - 7 |
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ISSN: |
0960-8524 |
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DOI: |
https://doi.org/10.1016/j.biortech.2020.123616 |
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Idioma: |
Inglês |
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Conteúdo: |
Trichoderma harzianum has attracting attention for its potential alternative use in biofuel production, due to a recognized competence for high diversity glycoside hydrolases (GH) enzyme complex, including higher β-glucosidases and auxiliary proteins, using low-cost carbon sources. This strain constitutively overexpressed the global regulator putative methyltransferase - LAE1, in order to improve the GHs production. The recombinant strain achieved 79-fold increase in lae1 expression and high GHs productivity. The evaluation of the LAE1 impact to induce the GHs used soluble and lignocellulose inexpensive carbon sources in a stirred-tank bioreactor. Using sugarcane bagasse with sucrose, the overexpression of lae1 resulted in significantly increment of gh61b (31x), cel7a (25x), bgl1(20x) and xyn3 (20x) genes expression. Reducing sugar released from pretreated sugarcane bagasse, which hydrolyzed by recombinant crude enzyme cocktail, achieved 41% improvement. Therefore, lae1 overexpression effectively is a promising improving GHs target for biomass degradation by T. harzianum. |
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Palavras-Chave: |
Glycoside hydrolases; LAE1; Overexpression. |
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Categoria do assunto: |
-- |
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Marc: |
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001 2141187
005 2022-06-24
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022 $a0960-8524
024 7 $ahttps://doi.org/10.1016/j.biortech.2020.123616$2DOI
100 1 $aDELABONA, P. S.
245 $aThe impact of putative methyltransferase overexpression on the Trichoderma harzianum cellulolytic system for biomass conversion.$h[electronic resource]
260 $c2022
300 $a1 - 7
520 $aTrichoderma harzianum has attracting attention for its potential alternative use in biofuel production, due to a recognized competence for high diversity glycoside hydrolases (GH) enzyme complex, including higher β-glucosidases and auxiliary proteins, using low-cost carbon sources. This strain constitutively overexpressed the global regulator putative methyltransferase - LAE1, in order to improve the GHs production. The recombinant strain achieved 79-fold increase in lae1 expression and high GHs productivity. The evaluation of the LAE1 impact to induce the GHs used soluble and lignocellulose inexpensive carbon sources in a stirred-tank bioreactor. Using sugarcane bagasse with sucrose, the overexpression of lae1 resulted in significantly increment of gh61b (31x), cel7a (25x), bgl1(20x) and xyn3 (20x) genes expression. Reducing sugar released from pretreated sugarcane bagasse, which hydrolyzed by recombinant crude enzyme cocktail, achieved 41% improvement. Therefore, lae1 overexpression effectively is a promising improving GHs target for biomass degradation by T. harzianum.
653 $aGlycoside hydrolases
653 $aLAE1
653 $aOverexpression
700 1 $aCODIMA, C. A.
700 1 $aRAMONI, J.
700 1 $aZUBIETA, M. P.
700 1 $aARAÚJO, B. M. de
700 1 $aFARINAS, C. S.
700 1 $aPRADELLA, J. G. C.
700 1 $aSEIBOTH, B.
773 $tBioresource Technology$gv. 313, 12361, 2020.
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| 1. |  | DELABONA, P. S.; CODIMA, C. A.; RAMONI, J.; ZUBIETA, M. P.; ARAÚJO, B. M. de; FARINAS, C. S.; PRADELLA, J. G. C.; SEIBOTH, B. The impact of putative methyltransferase overexpression on the Trichoderma harzianum cellulolytic system for biomass conversion. Bioresource Technology, v. 313, 12361, 2020. 1 - 7| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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