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Registro Completo |
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Biblioteca(s): |
Embrapa Amapá. |
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Data corrente: |
21/02/2014 |
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Data da última atualização: |
20/03/2017 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
FERREIRA, D. S. da S.; ARAÚJO, B. H. P. de; RIBEIRO, G. G.; FARIAS, J. E. dos S.; GUEDES, M. C. |
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Afiliação: |
MARCELINO CARNEIRO GUEDES, CPAF-AP. |
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Título: |
Caracterização de clareiras em floresta de várzea no Estuário Amazônico. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: CONGRESSO INTERNACIONAL DE ECOLOGIA, 1.; CONGRESSO INTERNACIONAL DE ECOLOGIA DO BRASIL, 11., 2013, Porto Seguro. Ecologia e Gestão Ambiental: anais. Porto Seguro: SEB, 2013. |
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Idioma: |
Português |
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Conteúdo: |
Atribui-se a abertura de clareiras formadas pela queda de uma ou mais árvores do dossel como o principal mecanismo de manutenção da diversidade de árvores e arbustos em florestas tropicais (TABARELLI e MONTOVANI, 1999). A formação de clareiras pode ser causada pela queda de galhos, que gera menor abertura no dossel e destruição do sub-bosque; pela morte de árvores em pé, que têm suas copas fragmentadas aos poucos; e pelo desenraizamento das árvores, que além de gerarem destruição no sub-bosque, causam revolvimento do solo na região da raiz (LIMA, 2005). Embora seja evidente a importância do conhecimento sobre a queda de árvores e formação de clareiras para a dinâmica florestal, é notória a carência de estudos referentes a estes distúrbios em florestas de várzea, pois poucos estudos sobre o tema foram realizados nesses ecossistemas, além disso, os estudos sobre clareiras realizados no Brasil são escassos e pontuais (LIMA, 2005). O objetivo foi caracterizar clareiras recentes quanto a origem (natural ou antrópica) e verificar e a abertura da clareira e a altura do solo é diretamente proporcional a abertura do dossel. |
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Palavras-Chave: |
Dossel. |
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Thesagro: |
Aspecto fisiográfico; Vegetação. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97944/1/CPAF-AP-2013-Clareiras-florestas.pdf
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Marc: |
LEADER 01874nam a2200193 a 4500
001 1980883
005 2017-03-20
008 2013 bl uuuu u00u1 u #d
100 1 $aFERREIRA, D. S. da S.
245 $aCaracterização de clareiras em floresta de várzea no Estuário Amazônico.$h[electronic resource]
260 $aIn: CONGRESSO INTERNACIONAL DE ECOLOGIA, 1.; CONGRESSO INTERNACIONAL DE ECOLOGIA DO BRASIL, 11., 2013, Porto Seguro. Ecologia e Gestão Ambiental: anais. Porto Seguro: SEB$c2013
520 $aAtribui-se a abertura de clareiras formadas pela queda de uma ou mais árvores do dossel como o principal mecanismo de manutenção da diversidade de árvores e arbustos em florestas tropicais (TABARELLI e MONTOVANI, 1999). A formação de clareiras pode ser causada pela queda de galhos, que gera menor abertura no dossel e destruição do sub-bosque; pela morte de árvores em pé, que têm suas copas fragmentadas aos poucos; e pelo desenraizamento das árvores, que além de gerarem destruição no sub-bosque, causam revolvimento do solo na região da raiz (LIMA, 2005). Embora seja evidente a importância do conhecimento sobre a queda de árvores e formação de clareiras para a dinâmica florestal, é notória a carência de estudos referentes a estes distúrbios em florestas de várzea, pois poucos estudos sobre o tema foram realizados nesses ecossistemas, além disso, os estudos sobre clareiras realizados no Brasil são escassos e pontuais (LIMA, 2005). O objetivo foi caracterizar clareiras recentes quanto a origem (natural ou antrópica) e verificar e a abertura da clareira e a altura do solo é diretamente proporcional a abertura do dossel.
650 $aAspecto fisiográfico
650 $aVegetação
653 $aDossel
700 1 $aARAÚJO, B. H. P. de
700 1 $aRIBEIRO, G. G.
700 1 $aFARIAS, J. E. dos S.
700 1 $aGUEDES, M. C.
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Registro original: |
Embrapa Amapá (CPAF-AP) |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
19/04/2023 |
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Data da última atualização: |
19/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
RIEGER, J. S. G.; MANTOVANI, C.; SUNIGA, P. A. P.; PINTO, I. B.; SOUSA, G. A. A.; GAVA, D.; CANTAO, M. E.; SANTOS, L. R.; ARAÚJO, F. R.; ZANELLA, J. R. C. |
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Afiliação: |
DANIELLE GAVA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; JANICE REIS CIACCI ZANELLA, CNPSA. |
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Título: |
Standardization of ELISA with senecavirus A recombinant VP2 protein and its use in swine herds in Brazil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 22, n. 1, ed. gmr19118, 2023. |
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DOI: |
http://dx.doi.org/10.4238/gmr19118 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here was able to detect specific SVA antibodies in acute disease and recovered pigs, and no cross-reactivity with VSV was observed. This assay has potential as a useful tool for monitoring SVA infection and could help to improve disease diagnosis. MenosAbstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here wa... Mostrar Tudo |
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Thesagro: |
Diagnostico. |
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Thesaurus NAL: |
Senecavirus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1153239/1/final10100.pdf
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Marc: |
LEADER 02545naa a2200265 a 4500
001 2153239
005 2023-04-19
008 2023 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/gmr19118$2DOI
100 1 $aRIEGER, J. S. G.
245 $aStandardization of ELISA with senecavirus A recombinant VP2 protein and its use in swine herds in Brazil.$h[electronic resource]
260 $c2023
520 $aAbstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here was able to detect specific SVA antibodies in acute disease and recovered pigs, and no cross-reactivity with VSV was observed. This assay has potential as a useful tool for monitoring SVA infection and could help to improve disease diagnosis.
650 $aSenecavirus
650 $aDiagnostico
700 1 $aMANTOVANI, C.
700 1 $aSUNIGA, P. A. P.
700 1 $aPINTO, I. B.
700 1 $aSOUSA, G. A. A.
700 1 $aGAVA, D.
700 1 $aCANTAO, M. E.
700 1 $aSANTOS, L. R.
700 1 $aARAÚJO, F. R.
700 1 $aZANELLA, J. R. C.
773 $tGenetics and Molecular Research$gv. 22, n. 1, ed. gmr19118, 2023.
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Embrapa Suínos e Aves (CNPSA) |
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