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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
15/12/2017 |
Data da última atualização: |
27/12/2017 |
Autoria: |
SALLES, M. G. F.; ARAÚJO, A. A. de. |
Título: |
Efeito macho na sincronização do estro de cabras Saanen no Nordeste do Brasil. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
In: SIMPÓSIO INTERNACIONAL SOBRE CAPRINOS E OVINOS DE CORTE, 3., 2007, João Pessoa. Anais... João Pessoa: SEBRAE-PB: EMEPA-PB, 2007. 3 f. 1 CD-ROM. |
Idioma: |
Português |
Conteúdo: |
Neste trabalho objetivou-se avaliar o uso do efeito macho na indução e sincronização do estro em 547 cabras leiteiras Saanen durante sete anos consecutivos, criadas em Pacatuba-CE. Os resultados mostram uma evolução no emprego deste método com 72,21%, 72,91%,74,02%, 77,46%, 83,42%, 87,36%, 86,66% de parições nos sete anos consecutivos, mostrando ser um método eficiente e de baixo para a indução e sincronização do estro em cabras leiteiras. Abstract: This work aimed to evaluate the use of male effect for seven consecutives years on the induction and synchronization of estrus in Five hundred and forty-seven Saanen goats raised in the Pacatuba-CE. The results shows an evolution of the use of male effect with 72,21%, 72,91%,74,02%, 77,46%, 83,42%, 87,36%, 86,66% of kidding rates during the seven successive years. The efficiency and the low cost presented by the male effect show its viability for the induction and synchronization of estrus in milk goats. |
Palavras-Chave: |
Efeito macho. |
Thesagro: |
Cabra leiteira; Caprino; Ciclo estral; Sincronização do cio. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 01620naa a2200193 a 4500 001 2082693 005 2017-12-27 008 2007 bl uuuu u00u1 u #d 100 1 $aSALLES, M. G. F. 245 $aEfeito macho na sincronização do estro de cabras Saanen no Nordeste do Brasil. 260 $c2007 520 $aNeste trabalho objetivou-se avaliar o uso do efeito macho na indução e sincronização do estro em 547 cabras leiteiras Saanen durante sete anos consecutivos, criadas em Pacatuba-CE. Os resultados mostram uma evolução no emprego deste método com 72,21%, 72,91%,74,02%, 77,46%, 83,42%, 87,36%, 86,66% de parições nos sete anos consecutivos, mostrando ser um método eficiente e de baixo para a indução e sincronização do estro em cabras leiteiras. Abstract: This work aimed to evaluate the use of male effect for seven consecutives years on the induction and synchronization of estrus in Five hundred and forty-seven Saanen goats raised in the Pacatuba-CE. The results shows an evolution of the use of male effect with 72,21%, 72,91%,74,02%, 77,46%, 83,42%, 87,36%, 86,66% of kidding rates during the seven successive years. The efficiency and the low cost presented by the male effect show its viability for the induction and synchronization of estrus in milk goats. 650 $aCabra leiteira 650 $aCaprino 650 $aCiclo estral 650 $aSincronização do cio 653 $aEfeito macho 700 1 $aARAÚJO, A. A. de 773 $tIn: SIMPÓSIO INTERNACIONAL SOBRE CAPRINOS E OVINOS DE CORTE, 3., 2007, João Pessoa. Anais... João Pessoa: SEBRAE-PB: EMEPA-PB, 2007. 3 f. 1 CD-ROM.
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Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca(s): |
Embrapa Agrossilvipastoril. |
Data corrente: |
03/02/2014 |
Data da última atualização: |
10/08/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
RODRIGUES, M. B. C.; FAVARO, L. C. de L.; PALLU, A. P. de S.; FERREIRA, A.; SEBASTIANES, F. de S.; RODRIGUES, M. J. C.; SPÓSITO, M. B.; ARAÚJO, W. L. de; PIZZIRANI-KLEINER, A. A. |
Afiliação: |
Maria Beatriz Calderan Rodrigues, USP-ESALQ; LEIA CECILIA DE LIMA FAVARO, CNPAE; Ana Paula de Souza Pallu, USP-ESALQ; ANDERSON FERREIRA, CPAMT; Fernanda de Souza Sebastianes, USP-ESALQ; Maria Juliana Calderan Rodrigues, USP-ESALQ; Marcel Bellato Spósito, USP-ESALQ; Welington Luiz DE Araújo, UMC; Aline Aparecida Pizzirani-Kleiner, USP-ESALQ. |
Título: |
Agrobacterium-mediated transformation of Guignardia citricarpa: an efficient tool to gene transfer and random mutagenesis. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Fungal Biology, Oxford, v. 117, n. 7/8, p. 556-568, jul./aug., 2013. |
ISSN: |
1878-6146 |
DOI: |
http://dx.doi.org/10.1016/j.funbio.2013.06.005 |
Idioma: |
Inglês |
Conteúdo: |
Guignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control CBS. This work describes for the first time an efficient and successful methodology for genetic transformation of G. citricarpa mycelia, which generated transformants expressing the gene encoding for the gfp (green fluorescent protein) and also their interaction with citrus plant. Mycelia of G. citricarpa were transformed via Agrobacterium tumefaciens, which carried the plasmid pFAT-gfp, contains the genes for hygromycin resistance (hph) as well as gfp. The optimization of the agrotransformation protocol was performed testing different conditions (type of membrane; inductor agent concentration [acetosyringonee - AS] and cocultivation time). Results demonstrated that the best condition occurred with the utilization of cellulose?s ester membrane; 200µM of AS and 96 h as cocultivation time. High mitotic stability (82 %) was displayed by transformants using Polymerase Chain Reaction (PCR) technique to confirm the hph gene insertion. In addition, the presence of gfp was observed inside mycelia by epifluorescence optical microscopy. This technique easy visualization of the behaviour of the pathogen interacting with the plant for the first time, allowing future studies on the pathogenesis of this fungus. The establishment of a transformation method for G. citricarpa opens a range of possibilities and facilitates the study of insertional mutagenesis and genetic knockouts, in order to identify the most important genes involved in the pathogenesis mechanisms and plant-pathogen interaction. MenosGuignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control CBS. This work describes for the first time an efficient and successful methodology for genetic transformation of G. citricarpa mycelia, which generated transformants expressing the gene encoding for the gfp (green fluorescent protein) and also their interaction with citrus plant. Mycelia of G. citricarpa were transformed via Agrobacterium tumefaciens, which carried the plasmid pFAT-gfp, contains the genes for hygromycin resistance (hph) as well as gfp. The optimization of the agrotransformation protocol was performed testing different conditions (type of membrane; inductor agent concentration [acetosyringonee - AS] and cocultivation time). Results demonstrated that the best condition occurred with the utilization of cellulose?s ester membrane; 200µM of AS and 96 h as cocultivation time. High mitotic stability (82 %) was displayed by transformants using Polymerase Chain Reaction (PCR) technique to confirm the hph gene insertion. In addition, the presence of gfp was observed inside mycelia by epifluorescence optical microscopy. This technique easy visualization of the behaviour of the pathogen interacting with the plant for ... Mostrar Tudo |
Palavras-Chave: |
Agrobacterium-Mediated; Plant-Pathogen Interaction. |
Thesaurus NAL: |
citrus black spot; genetic transformation; Guignardia citricarpa. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/96268/1/cpamt-Ferreira-1878-6146-2013.pdf
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Marc: |
LEADER 02874naa a2200301 a 4500 001 1978312 005 2017-08-10 008 2013 bl uuuu u00u1 u #d 022 $a1878-6146 024 7 $ahttp://dx.doi.org/10.1016/j.funbio.2013.06.005$2DOI 100 1 $aRODRIGUES, M. B. C. 245 $aAgrobacterium-mediated transformation of Guignardia citricarpa$ban efficient tool to gene transfer and random mutagenesis.$h[electronic resource] 260 $c2013 520 $aGuignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control CBS. This work describes for the first time an efficient and successful methodology for genetic transformation of G. citricarpa mycelia, which generated transformants expressing the gene encoding for the gfp (green fluorescent protein) and also their interaction with citrus plant. Mycelia of G. citricarpa were transformed via Agrobacterium tumefaciens, which carried the plasmid pFAT-gfp, contains the genes for hygromycin resistance (hph) as well as gfp. The optimization of the agrotransformation protocol was performed testing different conditions (type of membrane; inductor agent concentration [acetosyringonee - AS] and cocultivation time). Results demonstrated that the best condition occurred with the utilization of cellulose?s ester membrane; 200µM of AS and 96 h as cocultivation time. High mitotic stability (82 %) was displayed by transformants using Polymerase Chain Reaction (PCR) technique to confirm the hph gene insertion. In addition, the presence of gfp was observed inside mycelia by epifluorescence optical microscopy. This technique easy visualization of the behaviour of the pathogen interacting with the plant for the first time, allowing future studies on the pathogenesis of this fungus. The establishment of a transformation method for G. citricarpa opens a range of possibilities and facilitates the study of insertional mutagenesis and genetic knockouts, in order to identify the most important genes involved in the pathogenesis mechanisms and plant-pathogen interaction. 650 $acitrus black spot 650 $agenetic transformation 650 $aGuignardia citricarpa 653 $aAgrobacterium-Mediated 653 $aPlant-Pathogen Interaction 700 1 $aFAVARO, L. C. de L. 700 1 $aPALLU, A. P. de S. 700 1 $aFERREIRA, A. 700 1 $aSEBASTIANES, F. de S. 700 1 $aRODRIGUES, M. J. C. 700 1 $aSPÓSITO, M. B. 700 1 $aARAÚJO, W. L. de 700 1 $aPIZZIRANI-KLEINER, A. A. 773 $tFungal Biology, Oxford$gv. 117, n. 7/8, p. 556-568, jul./aug., 2013.
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