Data/hora: 10/06/2024 18:24:22

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 02/02/2017
Data da última atualização: | 30/01/2023
Autoria: | GOLO, P. S.; SANTOS, A. S. de O. dos; MONTEIRO, C. M. O.; PERINOTTO, W. M. de S.; QUINELATO, S.; CAMARGO, M. G.; SÁ, F. A. de; ANGELO, I. da C.; MARTINS, M. F.; PRATA, M. C. de A.; BITTENCOURT, V. R. E. P.
Afiliação: | Patrícia Silva Golo, UFRRJ; Alessa Siqueira de Oliveira dos Santos; Caio Marcio Oliveira Monteiro, UFRRJ; Wendell Marcelo de Souza Perinotto, Universidade de Cuiabá; Simone Quinelato, Fundação Osvaldo Cruz; Mariana Guedes Camargo, UFRRJ; Fillipe Araujo de Sá, UFRJ; Isabele da Costa Angelo, UFRRJ; MARTA FONSECA MARTINS, CNPGL; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; Vânia Rita Elias Pinheiro Bittencourt, UFRRJ.
Título: | Lab-on-a-chip and SDS-PAGE analysis of hemolymph protein profile from Rhipicephalus microplus (Acari: Ixodidae) infected with entomopathogenic nematode and fungus.
Ano de publicação: | 2016
Fonte/Imprenta: | Parasitology Research, n. 115, p. 3459-3468, 2016.
Idioma: | Português
Conteúdo: | Abstract In the present study, lab-on-a-chip electrophoresis (LoaC) was suggested as an alternative method to the conventional polyacrylamide gel electrophoresis under denaturing conditions (SDS-PAGE) to analyze raw cell-free tick hemolymph. Rhipicephalus microplus females were exposed to the entomopathogenic fungus Metarhizium anisopliae senso latu IBCB 116 strain and/or to the entomopathogenic nematode Heterorhabditis indica LPP1 strain. Hemolymph from not exposed or exposed ticks was collected 16 and 24 h after exposure and analyze by SDS-PAGE or LoaC. SDS-PAGE yielded 15 bands and LoaC electrophoresis 17 bands. Despite the differences in the number of bands, when the hemolymph protein profiles of exposed or unexposed ticks were compared in the same method, no suppressing or additional bandswere detected among the treatments regardless themethod (i.e., SDS-PAGE or chip electrophoresis using the Protein 230 Kit®). The potential of LoaC electrophoresis to detect protein bands from tick hemolymph was considered more efficient in comparison to the detection obtained using the traditional SDS-PAGE method, especially when it comes to protein subunits heavier than 100 KDa. LoaC electrophoresis provided a very good reproducibility, and is much faster than the conventional SDS-PAGE method, which requires several hours for one analysis. Despite both methods can be used to analyze tick hemolymph composition, LoaC was consideredmore suitable for cell-free hemolymph protein separation and detection. LoaC hemolymph band percent data reported changes in key proteins (i.e., HeLp and vitellogenin) exceptionally important for tick embryogenesis. This study reported, for the first time, tick hemolymph protein profile using LoaC.
Palavras-Chave: | Cattle tick; Protein bands.
Thesaurus Nal: | biological control; gel electrophoresis; Heterorhabditis; Metarhizium.
Categoria do assunto: | O Insetos e Entomologia
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (23081-1) - UPC - AP - DD -  -  -  - DIS