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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
18/07/2022 |
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Data da última atualização: |
31/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MUDO, L. M. D.; QUEIROZ, A. F. S.; MELO, N. F. de; BARBOSA, M. A. G.; ANDRADE, E. C. de; BRITTO, D. de. |
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Afiliação: |
LAISLANE MIRELA DESMONDE MUDO, UNIVASF; AGDA FREIRE SILVA QUEIROZ, IF SERTÃO de Pernambuco; NATONIEL FRANKLIN DE MELO, CPATSA; MARIA ANGELICA GUIMARAES BARBOSA, CPATSA; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; DOUGLAS DE BRITTO, CPATSA. |
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Título: |
Stability evaluation of dsRNA and DNA encapsulated in chitosan nanoparticles; |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
BioNanoScience, v. 12, p. 774-784, 2022. |
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DOI: |
https://doi.org/10.1007/s12668-022-01003-y |
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Idioma: |
Inglês |
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Conteúdo: |
The polyelectrolyte efect of polysaccharides can be exploited in biotechnological systems for the encapsulation and transport of nucleic acids (NA). The main target applications could be non-viral vaccines and gene silencing. As general application models, this study used plasmid DNA from Xanthomonas citri pv. viticola and dsRNA molecules. Encapsulation occurred by ionotropic gelation based on the pre- and post-addition of NA to the nanoparticle. The suspensions were characterized by UV?visible spectroscopy, electrophoresis, particle size and zeta potential (dynamic light scattering, DLS), and scanning electronic microscopy (SEM). Size distribution was around 173 nm and 587 nm, respectively, for dsRNA and DNA, according to the DLS technique. The post-addition process resulted in small nanoparticles and a more stable suspension, with high zeta potential. The SEM analysis showed spherical nanoparticle and homogeneity in distribution. The degradation study showed that the encapsulation of both dsRNA and DNA resulted in stable nanoparticles when subjected to 80 °C, ultraviolet light, and the action of the RNase enzyme (in the case of dsRNA). Furthermore, stability was still efective in the solid state through the freeze-drying and casting processes. Therefore, dsRNA and DNA nanoencapsulated by ionotropic gelation are feasible, with a high potential for phytopathological application in disease treatment and pest control. |
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Palavras-Chave: |
Cancro bacteriano da videira; Degradação de RNA e DNA; Efeito elicitor; Encapsulamento; Nanoparticula; Nanotecnologia; Quitosana. |
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Thesagro: |
Cancro Bacteriano; Doença; Uva; Xanthomonas Campestris. |
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Thesaurus Nal: |
Encapsulation; Grapes. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02439naa a2200349 a 4500
001 2144770
005 2023-01-31
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s12668-022-01003-y$2DOI
100 1 $aMUDO, L. M. D.
245 $aStability evaluation of dsRNA and DNA encapsulated in chitosan nanoparticles;$h[electronic resource]
260 $c2022
520 $aThe polyelectrolyte efect of polysaccharides can be exploited in biotechnological systems for the encapsulation and transport of nucleic acids (NA). The main target applications could be non-viral vaccines and gene silencing. As general application models, this study used plasmid DNA from Xanthomonas citri pv. viticola and dsRNA molecules. Encapsulation occurred by ionotropic gelation based on the pre- and post-addition of NA to the nanoparticle. The suspensions were characterized by UV?visible spectroscopy, electrophoresis, particle size and zeta potential (dynamic light scattering, DLS), and scanning electronic microscopy (SEM). Size distribution was around 173 nm and 587 nm, respectively, for dsRNA and DNA, according to the DLS technique. The post-addition process resulted in small nanoparticles and a more stable suspension, with high zeta potential. The SEM analysis showed spherical nanoparticle and homogeneity in distribution. The degradation study showed that the encapsulation of both dsRNA and DNA resulted in stable nanoparticles when subjected to 80 °C, ultraviolet light, and the action of the RNase enzyme (in the case of dsRNA). Furthermore, stability was still efective in the solid state through the freeze-drying and casting processes. Therefore, dsRNA and DNA nanoencapsulated by ionotropic gelation are feasible, with a high potential for phytopathological application in disease treatment and pest control.
650 $aEncapsulation
650 $aGrapes
650 $aCancro Bacteriano
650 $aDoença
650 $aUva
650 $aXanthomonas Campestris
653 $aCancro bacteriano da videira
653 $aDegradação de RNA e DNA
653 $aEfeito elicitor
653 $aEncapsulamento
653 $aNanoparticula
653 $aNanotecnologia
653 $aQuitosana
700 1 $aQUEIROZ, A. F. S.
700 1 $aMELO, N. F. de
700 1 $aBARBOSA, M. A. G.
700 1 $aANDRADE, E. C. de
700 1 $aBRITTO, D. de
773 $tBioNanoScience$gv. 12, p. 774-784, 2022.
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| 7. |  | ANDRADE, E. B. de. Indução de mutação. Belém, PA: IPEAN, 1973. 10 p. Apresentado no Seminário Técnico realizado em marco de 1973. Publicação não convencional. Mimeografado.| Biblioteca(s): Embrapa Amazônia Oriental. |
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