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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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Data corrente: |
07/07/2022 |
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Data da última atualização: |
08/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TÁVORA, F. T. P. K.; DINIZ, F. de A. dos S.; RÊGO-MACHADO, C. de M.; FREITAS, N. C.; ARRAES, F. B. M.; ANDRADE, E. C. de; FURTADO, L. L.; OSIRO, K. O.; SOUSA, N. L. de; CARDOSO, T. B.; MERTZ-HENNING, L. M.; OLIVEIRA, P. A. de; FEINGOLD, S. E.; HUNTER, W. B.; SA, M. F. G. de; KOBAYASHI, A. K.; NEPOMUCENO, A. L.; SANTIAGO, T. R.; MOLINARI, H. B. C. |
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Afiliação: |
FABIANO TOUZDJIAN PINHEIRO KOHLRAUSCH TÁVORA, UNB; FRANCISCO DE ASSIS DOS SANTOS DINIZ, UNB; CAMILA DE MORAES RÊGO-MACHADO, UNB; NATÁLIA CHAGAS FREITAS; FABRÍCIO BARBOSA MONTEIRO ARRAES; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; LEILA LOURENÇO FURTADO, UNB; KAREN OFUJI OSIRO, UNB; NATÁLIA LIMA DE SOUSA, IPADS-Balcarce (UEDD INTA-CONICET), Argentina; THIAGO BÉRGAMO CARDOSO, SEMPRE AgTech; LILIANE MARCIA MERTZ HENNING, CNPSO; PATRICIA ABRAO DE OLIVEIRA MOLINARI, CNPAE; SÉRGIO ENRIQUE FEINGOLD, IPADS-Balcarce (UEDD INTA-CONICET), Argentina; WAYNE B. HUNTER, USDA-ARS, United States; MARIA FATIMA GROSSI DE SA, Cenargen; ADILSON KENJI KOBAYASHI, CNPAE; ALEXANDRE LIMA NEPOMUCENO, CNPSO; THAÍS RIBEIRO SANTIAGO, UNB; HUGO BRUNO CORREA MOLINARI, SEMPRE AgTech. |
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Título: |
CRISPR/Cas- and Topical RNAi-Based Technologies for crop management and improvement: reviewing the risk assessment and challenges towards a more sustainable agriculture. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Frontiers in Bioengineering and Biotechnology, v. 10, 2022. Article 913728. |
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DOI: |
https://doi.org/10.3389/fbioe.2022.913728 |
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Idioma: |
Inglês |
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Conteúdo: |
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture, introducing or improving important agronomic traits in plant crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, the limitations of each technique, public perception, and regulatory aspects are hindering its wide adoption for the development of new crop varieties or products. In an attempt to reverse these mishaps, scientists have been researching alternatives to increase the specificity, uptake, and stability of the CRISPR and RNAi system components in the target organism, as well as to reduce the chance of toxicity in nontarget organisms to minimize environmental risk, health problems, and regulatory issues. In this review, we discuss several aspects related to risk assessment, toxicity, and advances in the use of CRISPR/Cas and topical RNAi-based technologies in crop management and breeding. The present study also highlights the advantages and possible drawbacks of each technology, provides a brief overview of how to circumvent the off-target occurrence, the strategies to increase on-target specificity, the harm/benefits of association with nanotechnology, the public perception of the available techniques, worldwide regulatory frameworks regarding topical RNAi and CRISPR technologies, and, lastly, presents successful case studies of biotechnological solutions derived from both technologies, raising potential challenges to reach the market and being social and environmentally safe. MenosClustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture, introducing or improving important agronomic traits in plant crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, the limitations of each technique, public perception, and regulatory aspects are hindering its wide adoption for the development of new crop varieties or products. In an attempt to reverse these mishaps, scientists have been researching alternatives to increase the specificity, uptake, and stability of the CRISPR and RNAi system components in the target organism, as well as to reduce the chance of toxicity in nontarget organisms to minimize environmental risk, health problems, and regulatory issues. In this review, we discuss several aspects related to risk assessment, toxicity, and advances in the use of CRISPR/Cas and topical RNAi-based technologies in crop management and breeding. The present study also highlights the advantages and possible drawbacks of each technology, provides a brief overview of how to circumvent the off-target occurrence, the strategies to increase on-target specificity, the harm/benefits of association with nanotechnology, the public pe... Mostrar Tudo |
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Palavras-Chave: |
Exogenous dsRNA; Genome editin; Genome editing; Offtargets; Public acceptance; Regulatory aspects. |
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Thesagro: |
Genoma. |
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Thesaurus Nal: |
Gene silencing; Nanotechnology; Toxicity. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03276naa a2200469 a 4500
001 2144523
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008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fbioe.2022.913728$2DOI
100 1 $aTÁVORA, F. T. P. K.
245 $aCRISPR/Cas- and Topical RNAi-Based Technologies for crop management and improvement$breviewing the risk assessment and challenges towards a more sustainable agriculture.$h[electronic resource]
260 $c2022
520 $aClustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture, introducing or improving important agronomic traits in plant crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, the limitations of each technique, public perception, and regulatory aspects are hindering its wide adoption for the development of new crop varieties or products. In an attempt to reverse these mishaps, scientists have been researching alternatives to increase the specificity, uptake, and stability of the CRISPR and RNAi system components in the target organism, as well as to reduce the chance of toxicity in nontarget organisms to minimize environmental risk, health problems, and regulatory issues. In this review, we discuss several aspects related to risk assessment, toxicity, and advances in the use of CRISPR/Cas and topical RNAi-based technologies in crop management and breeding. The present study also highlights the advantages and possible drawbacks of each technology, provides a brief overview of how to circumvent the off-target occurrence, the strategies to increase on-target specificity, the harm/benefits of association with nanotechnology, the public perception of the available techniques, worldwide regulatory frameworks regarding topical RNAi and CRISPR technologies, and, lastly, presents successful case studies of biotechnological solutions derived from both technologies, raising potential challenges to reach the market and being social and environmentally safe.
650 $aGene silencing
650 $aNanotechnology
650 $aToxicity
650 $aGenoma
653 $aExogenous dsRNA
653 $aGenome editin
653 $aGenome editing
653 $aOfftargets
653 $aPublic acceptance
653 $aRegulatory aspects
700 1 $aDINIZ, F. de A. dos S.
700 1 $aRÊGO-MACHADO, C. de M.
700 1 $aFREITAS, N. C.
700 1 $aARRAES, F. B. M.
700 1 $aANDRADE, E. C. de
700 1 $aFURTADO, L. L.
700 1 $aOSIRO, K. O.
700 1 $aSOUSA, N. L. de
700 1 $aCARDOSO, T. B.
700 1 $aMERTZ-HENNING, L. M.
700 1 $aOLIVEIRA, P. A. de
700 1 $aFEINGOLD, S. E.
700 1 $aHUNTER, W. B.
700 1 $aSA, M. F. G. de
700 1 $aKOBAYASHI, A. K.
700 1 $aNEPOMUCENO, A. L.
700 1 $aSANTIAGO, T. R.
700 1 $aMOLINARI, H. B. C.
773 $tFrontiers in Bioengineering and Biotechnology$gv. 10, 2022. Article 913728.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Biblioteca |
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Registro |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
24/09/2015 |
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Data da última atualização: |
07/03/2016 |
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Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
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Autoria: |
ALMEIDA, C. O. de; SOUZA, C. da C. |
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Afiliação: |
CLOVIS OLIVEIRA DE ALMEIDA, CNPMF; CARINE DA CONCEIÇÃO SOUZA. |
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Título: |
Custo-Embrapa de seleção avançada de variedades de mandioca resistentes à podridão radicular. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2015. |
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Páginas: |
15 p. |
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Série: |
(Embrapa Mandioca e Fruticultura. Boletim de Pesquisa e Desenvolvimento, 70). |
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ISSN: |
1809-5003 |
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Idioma: |
Português |
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Conteúdo: |
Nesta publicação, foi calculado o custo da Embrapa com ensaios avançados de seleção de variedades de mandioca resistentes à podridão radicular. Os testes agronômicos em campo foram realizados em 17 municípios do Estado de Sergipe, no período de 1997 a 2002. De um total de 4.000 híbridos avaliados, apenas dois foram selecionados. Os custos com pessoal responderam pela maior parcela dos custos totais. |
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Palavras-Chave: |
Plant disease. |
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Thesagro: |
Doença de planta; Mandioca. |
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Thesaurus NAL: |
Cassava. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/130288/1/BoletimPesquisa70-261-14Publica-Clovis.pdf
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Marc: |
LEADER 01085nam a2200205 a 4500
001 2024976
005 2016-03-07
008 2015 bl uuuu u0uu1 u #d
022 $a1809-5003
100 1 $aALMEIDA, C. O. de
245 $aCusto-Embrapa de seleção avançada de variedades de mandioca resistentes à podridão radicular.$h[electronic resource]
260 $aCruz das Almas, BA: Embrapa Mandioca e Fruticultura$c2015
300 $a15 p.
490 $a(Embrapa Mandioca e Fruticultura. Boletim de Pesquisa e Desenvolvimento, 70).
520 $aNesta publicação, foi calculado o custo da Embrapa com ensaios avançados de seleção de variedades de mandioca resistentes à podridão radicular. Os testes agronômicos em campo foram realizados em 17 municípios do Estado de Sergipe, no período de 1997 a 2002. De um total de 4.000 híbridos avaliados, apenas dois foram selecionados. Os custos com pessoal responderam pela maior parcela dos custos totais.
650 $aCassava
650 $aDoença de planta
650 $aMandioca
653 $aPlant disease
700 1 $aSOUZA, C. da C.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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