| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
22/02/2010 |
|
Data da última atualização: |
11/08/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SIQUEIRA, L. G. B.; TORRES, C. A. A.; AMORIM, L. S.; SOUZA, E. D.; CAMARGO, L. S. de A.; FERNANDES, C. A. C.; VIANA, J. H. M. |
|
Afiliação: |
LUIZ GUSTAVO B. SIQUEIRA, UFV; CIRO A. A. TORRES, UFV; LINCOLN S. AMORIM, UFV; ELIZA D. SOUZA; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; CARLOS A. C. FERNANDES, Universidade de Alfenas; JOAO HENRIQUE MOREIRA VIANA, CNPGL. |
|
Título: |
Interrelationships among morphology, echotexture, and function of the bovine corpus luteum during the estrous cycle. |
|
Ano de publicação: |
2009 |
|
Fonte/Imprenta: |
Animal Reproduction Science, v. 115, n. 1/4, p. 18-28, 2009. |
|
DOI: |
https://doi.org/10.1016/j.anireprosci.2008.11.009 |
|
Idioma: |
Inglês |
|
Conteúdo: |
It has been suggested that ultrasound image attributes are a potential indicator of physiological and functional status of the corpus luteum (CL) in several species, including cattle. The aims of this study were to evaluate CL morphological, functional and echotextural characteristics, and also to investigate the hypothesis that those attributes are correlated and change similarly throughout an estrous cycle. Ovaries of crossbred (Bos taurus taurus x Bos taurus indicus) heifers were evaluated using ultrasonography daily throughout an interestrus interval using a B-mode, real-time ultrasound machine equipped with a 5 MHz linear-array rectal transducer, during a natural estrous cycle (Experiment 1; n=12) or during a shortened cycle, with luteolysis induction 10d after estrus (Experiment 2; n=6). Blood samples were collected for assay of plasma progesterone concentrations. Corpora lutea areas were measured and daily images of each CL were videotaped and digitized for computer-assisted analysis using custom-developed software. In Experiment 1, area of luteal tissue increased until a maximum value 10d after estrus (P<0.001), followed by a plateau phase, and then a decline beginning 14 d after estrus. Luteal tissue area was highly correlated to plasma progesterone concentrations (r=0.86; P<0.001). When luteolysis was induced in Experiment 2, loss of CL function (decrease in plasma progesterone concentrations to metestrous values) preceded tissue regression by 48 h (24h compared with 72 h; P<0.001). The mean pixel value of ultrasound images did not change in Experiment 1 (P>0.70), but a day effect on this attribute was observed in Experiment 2 (P=0.052). In contrast, mean pixel value was correlated to plasma progesterone concentrations in Experiment 1 (r=-0.63; P<0.05), but not in Experiment 2 (r=-0.28; P>0.10). In regard to CL heterogeneity, defined as the standard deviation of the mean pixel value of the luteal tissue, a time effect was observed following both natural (Experiment 1; P<0.009) and luteolysis-induced (Experiment 2; P<0.05) estrous cycles (P<0.05). Moreover, this variable was correlated with plasma progesterone concentrations (r=-0.71 and -0.58 in Experiments 1 and 2, respectively; P<0.01), indicating that CL images were more heterogeneous during metestrus and after luteolysis (functional regression). In summary, morphological and echotextural attributes were correlated with CL function and underwent similar changes during the estrous cycle. Luteal tissue heterogeneity, assessed by ultrasonography, is considered a potential indicator of CL functional status, because it is correlated to circulating progesterone concentrations. MenosIt has been suggested that ultrasound image attributes are a potential indicator of physiological and functional status of the corpus luteum (CL) in several species, including cattle. The aims of this study were to evaluate CL morphological, functional and echotextural characteristics, and also to investigate the hypothesis that those attributes are correlated and change similarly throughout an estrous cycle. Ovaries of crossbred (Bos taurus taurus x Bos taurus indicus) heifers were evaluated using ultrasonography daily throughout an interestrus interval using a B-mode, real-time ultrasound machine equipped with a 5 MHz linear-array rectal transducer, during a natural estrous cycle (Experiment 1; n=12) or during a shortened cycle, with luteolysis induction 10d after estrus (Experiment 2; n=6). Blood samples were collected for assay of plasma progesterone concentrations. Corpora lutea areas were measured and daily images of each CL were videotaped and digitized for computer-assisted analysis using custom-developed software. In Experiment 1, area of luteal tissue increased until a maximum value 10d after estrus (P<0.001), followed by a plateau phase, and then a decline beginning 14 d after estrus. Luteal tissue area was highly correlated to plasma progesterone concentrations (r=0.86; P<0.001). When luteolysis was induced in Experiment 2, loss of CL function (decrease in plasma progesterone concentrations to metestrous values) preceded tissue regression by 48 h (24h compared wi... Mostrar Tudo |
|
Palavras-Chave: |
Cattle-ultrasonography; Echotexture; Heterogeneity; Pixel. |
|
Thesaurus Nal: |
animal reproduction; corpus luteum. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 03582naa a2200277 a 4500
001 1658399
005 2022-08-11
008 2009 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.anireprosci.2008.11.009$2DOI
100 1 $aSIQUEIRA, L. G. B.
245 $aInterrelationships among morphology, echotexture, and function of the bovine corpus luteum during the estrous cycle.$h[electronic resource]
260 $c2009
520 $aIt has been suggested that ultrasound image attributes are a potential indicator of physiological and functional status of the corpus luteum (CL) in several species, including cattle. The aims of this study were to evaluate CL morphological, functional and echotextural characteristics, and also to investigate the hypothesis that those attributes are correlated and change similarly throughout an estrous cycle. Ovaries of crossbred (Bos taurus taurus x Bos taurus indicus) heifers were evaluated using ultrasonography daily throughout an interestrus interval using a B-mode, real-time ultrasound machine equipped with a 5 MHz linear-array rectal transducer, during a natural estrous cycle (Experiment 1; n=12) or during a shortened cycle, with luteolysis induction 10d after estrus (Experiment 2; n=6). Blood samples were collected for assay of plasma progesterone concentrations. Corpora lutea areas were measured and daily images of each CL were videotaped and digitized for computer-assisted analysis using custom-developed software. In Experiment 1, area of luteal tissue increased until a maximum value 10d after estrus (P<0.001), followed by a plateau phase, and then a decline beginning 14 d after estrus. Luteal tissue area was highly correlated to plasma progesterone concentrations (r=0.86; P<0.001). When luteolysis was induced in Experiment 2, loss of CL function (decrease in plasma progesterone concentrations to metestrous values) preceded tissue regression by 48 h (24h compared with 72 h; P<0.001). The mean pixel value of ultrasound images did not change in Experiment 1 (P>0.70), but a day effect on this attribute was observed in Experiment 2 (P=0.052). In contrast, mean pixel value was correlated to plasma progesterone concentrations in Experiment 1 (r=-0.63; P<0.05), but not in Experiment 2 (r=-0.28; P>0.10). In regard to CL heterogeneity, defined as the standard deviation of the mean pixel value of the luteal tissue, a time effect was observed following both natural (Experiment 1; P<0.009) and luteolysis-induced (Experiment 2; P<0.05) estrous cycles (P<0.05). Moreover, this variable was correlated with plasma progesterone concentrations (r=-0.71 and -0.58 in Experiments 1 and 2, respectively; P<0.01), indicating that CL images were more heterogeneous during metestrus and after luteolysis (functional regression). In summary, morphological and echotextural attributes were correlated with CL function and underwent similar changes during the estrous cycle. Luteal tissue heterogeneity, assessed by ultrasonography, is considered a potential indicator of CL functional status, because it is correlated to circulating progesterone concentrations.
650 $aanimal reproduction
650 $acorpus luteum
653 $aCattle-ultrasonography
653 $aEchotexture
653 $aHeterogeneity
653 $aPixel
700 1 $aTORRES, C. A. A.
700 1 $aAMORIM, L. S.
700 1 $aSOUZA, E. D.
700 1 $aCAMARGO, L. S. de A.
700 1 $aFERNANDES, C. A. C.
700 1 $aVIANA, J. H. M.
773 $tAnimal Reproduction Science$gv. 115, n. 1/4, p. 18-28, 2009.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
|
Data corrente: |
30/06/2010 |
|
Data da última atualização: |
18/11/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
FERREIRA, C. R.; BURGSTALLER, J. P.; PERECIN, F.; GARCIA, J. M.; CHIARATTI, M. R.; MÉO, S. C.; MULLER, M.; SMITH, L. C.; MEIRELLES, F. V.; STEINBORN, R. |
|
Afiliação: |
CHRISTINA RAMIRES FERREIRA, UNESP-JABOTICABAL; JORG PATRICK BURGSTALLER, UNIVERSTIY OF VETERINARY MEDICINE - VIENNA; FELIPE PERECIN, UNESP - JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP - JABOTICABAL; MARCOS ROBERTO CHIARATTI, USP - PIRASSUNUNGA; SIMONE CRISTINA MÉO, CPPSE; MATHIAS MULLER, UNIVERSITY OF VETERINARY MEDICINE - VIENNA; LAWRENCE CHARLES SMITH, UNIVERSITE DE MONTREAL - QUEBEC; FLÁVIO VIEIRA MEIRELLES, USP - PIRASSUNUNGA; RALF STEINBORN, UNIVERSITY OF VETERINARY MEDICINE - VIENNA. |
|
Título: |
Pronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line. |
|
Ano de publicação: |
2010 |
|
Fonte/Imprenta: |
Biology of Reproduction, v. 82, n. 03, p. 563-571, mar. 2010. |
|
DOI: |
https://doi.org/10.1095/biolreprod.109.080564 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Ooplasmic transfer (01) has been used in basic mouse research for studying lhe segregation of mtDNA, as well as in human assisted reproduction for improving embryo development in cases of persistent developmental failure. Using cattle as a large-animal model, we demonstrate that lhe moderate amount of mitochondria introduced by OT is transmitted to lhe offspring's oocytes; e.g., modifies lhe germ fine. The danar mtDNA was detectable in 25% and 65% of oocytes collected from two females. Its high variation in heteroplasmic oocytes, ranging from 1.1% to 33.5% and from 0.4% to 15.5%, can be explained by random genetic drift in lhe female germ line. Centrifugation-mediated enrichment of mitochondria il1 lhe pole zone of lhe recipient zygote's ooplasm and its substitution by danar ooplasm led to elevated proportions of danar mtDNA in reconstructed zygotes compared with zygotes produced by standard OT (23.6% :!: 9.6% versus 12.1% :!: 4.5%; P <0.0001). We also characterized lhe proliferation of mitochondria from lhe OT parents-the recipient zygote (Bos primigenius taurus type) and lhe danar ooplasm (B. primigenius indicus type). Regression analysis performed for 57 tissue samples collected from lhe seven OT fetuses at different points during fetal development found a decreasing proportion of donor mt DNA (r2=0.78). This indicates a preferred proliferation of recipient of the OT recipeint expressinf a B. primigenius indicus phenotype. |
|
Palavras-Chave: |
Bovine; Developmental biology; Embryo; Gamete biology; Ooplasmic. |
|
Thesagro: |
DNA. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/28805/1/PROCISCMN2010.00002.pdf
|
|
Marc: |
LEADER 02370naa a2200313 a 4500
001 1856388
005 2022-11-18
008 2010 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1095/biolreprod.109.080564$2DOI
100 1 $aFERREIRA, C. R.
245 $aPronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line.$h[electronic resource]
260 $c2010
520 $aOoplasmic transfer (01) has been used in basic mouse research for studying lhe segregation of mtDNA, as well as in human assisted reproduction for improving embryo development in cases of persistent developmental failure. Using cattle as a large-animal model, we demonstrate that lhe moderate amount of mitochondria introduced by OT is transmitted to lhe offspring's oocytes; e.g., modifies lhe germ fine. The danar mtDNA was detectable in 25% and 65% of oocytes collected from two females. Its high variation in heteroplasmic oocytes, ranging from 1.1% to 33.5% and from 0.4% to 15.5%, can be explained by random genetic drift in lhe female germ line. Centrifugation-mediated enrichment of mitochondria il1 lhe pole zone of lhe recipient zygote's ooplasm and its substitution by danar ooplasm led to elevated proportions of danar mtDNA in reconstructed zygotes compared with zygotes produced by standard OT (23.6% :!: 9.6% versus 12.1% :!: 4.5%; P <0.0001). We also characterized lhe proliferation of mitochondria from lhe OT parents-the recipient zygote (Bos primigenius taurus type) and lhe danar ooplasm (B. primigenius indicus type). Regression analysis performed for 57 tissue samples collected from lhe seven OT fetuses at different points during fetal development found a decreasing proportion of donor mt DNA (r2=0.78). This indicates a preferred proliferation of recipient of the OT recipeint expressinf a B. primigenius indicus phenotype.
650 $aDNA
653 $aBovine
653 $aDevelopmental biology
653 $aEmbryo
653 $aGamete biology
653 $aOoplasmic
700 1 $aBURGSTALLER, J. P.
700 1 $aPERECIN, F.
700 1 $aGARCIA, J. M.
700 1 $aCHIARATTI, M. R.
700 1 $aMÉO, S. C.
700 1 $aMULLER, M.
700 1 $aSMITH, L. C.
700 1 $aMEIRELLES, F. V.
700 1 $aSTEINBORN, R.
773 $tBiology of Reproduction$gv. 82, n. 03, p. 563-571, mar. 2010.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
