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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
10/12/2018 |
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Data da última atualização: |
01/09/2023 |
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Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
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Autoria: |
NICIURA, S. C. M.; MORAES, C. V.; CRUVINEL, G. G.; ALEMÁN GAINZA, Y.; ALBUQUERQUE, A. C. A. de; SANTANA, R. C. M.; THOLON, P.; TIZIOTO, P. C.; CHAGAS, A. C. de S.; ESTEVES, S. N.; BENAVIDES, M. V.; AMARANTE, A. F. T. do. |
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Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; Caroline Valério Moraes, UFSCar; Giovanna Gabrielle Cruvinel, UNICEP; Yousmel Alemán Gainza, UNESP; Ana Cláudia Alexandre de Albuquerque, UNESP; RAUL COSTA MASCARENHAS SANTANA, CPPSE; PATRICIA THOLON, CPPSE; Polyana Cristine Tizioto, ESALQ; ANA CAROLINA DE SOUZA CHAGAS, CPPSE; SERGIO NOVITA ESTEVES, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; Alessandro Francisco Talamini do Amarante, UNESP. |
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Título: |
Análise genômica da resistência ao monepantel e investigação epigenética em Haemonchus contortus. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
São Carlos, SP: Embrapa Pecuária Sudeste, 2018. |
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Páginas: |
94 p. |
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Série: |
(Embrapa Pecuária Sudeste. Boletim de Pesquisa e Desenvolvimento, 43). |
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ISSN: |
1981-2078 |
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Idioma: |
Português |
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Conteúdo: |
Na ovinocultura, são grandes os prejuízos causados tanto por Haemonchus contortus quanto pela resistência desse parasita aos anti-helmínticos. Assim, no presente trabalho, foram investigados polimorfismos associados à resistência ao monepantel em H. contortus após sequenciamento genômico. Com taxas de mapeamento de 79,12% a 79,43% e cobertura de 64,54 a 71,22X, foram detectados Single Nucleotide Polymorphisms (SNPs) em éxons e variantes de alto impacto nos genes codificantes para: subunidade de receptor nicotínico de acetilcolina, transportadores ABC, glicoproteína-P, proteínas afetadas por outros anti-helmínticos, proteínas de canal de íons, peptidases, kinases e genes da maquinaria epigenética de modificação de histonas. Em análises de enriquecimento, destacaram-se genes envolvidos em locomoção, atividade de peptidase, canal de íons, transportador e receptor acoplado à proteína-G. Ainda, por investigação in silico, foram encontradas evidências da ausência de metilação do DNA em H. contortus. Dessa maneira, os polimorfismos identificados precisam ser investigados como marcadores moleculares de resistência ao monepantel e, assim, contribuírem para o diagnóstico precoce da resistência e monitoramento da eficácia de anti-helmínticos. Além disso, podem ser considerados potenciais alvos para o desenvolvimento de tratamentos alternativos ou de novos fármacos, incluindo os de efeito epigenético, para o controle de H. contortus em rebanhos ovinos. |
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Palavras-Chave: |
Gastrenterite; Introgressão de genes; Metilação de DNA; Nematoide grastrintestinal de ovinos; Resistência anti-helmíntica; Sequenciamento genômico. |
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Thesagro: |
Anti-Helmíntico; Genoma; Marcador Molecular; Metilação. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/255525/1/BOLETIM-42.pdf
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Marc: |
LEADER 02751nam a2200397 a 4500
001 2101016
005 2023-09-01
008 2018 bl uuuu 00u1 u #d
022 $a1981-2078
100 1 $aNICIURA, S. C. M.
245 $aAnálise genômica da resistência ao monepantel e investigação epigenética em Haemonchus contortus.$h[electronic resource]
260 $aSão Carlos, SP: Embrapa Pecuária Sudeste$c2018
300 $a94 p.
490 $a(Embrapa Pecuária Sudeste. Boletim de Pesquisa e Desenvolvimento, 43).
520 $aNa ovinocultura, são grandes os prejuízos causados tanto por Haemonchus contortus quanto pela resistência desse parasita aos anti-helmínticos. Assim, no presente trabalho, foram investigados polimorfismos associados à resistência ao monepantel em H. contortus após sequenciamento genômico. Com taxas de mapeamento de 79,12% a 79,43% e cobertura de 64,54 a 71,22X, foram detectados Single Nucleotide Polymorphisms (SNPs) em éxons e variantes de alto impacto nos genes codificantes para: subunidade de receptor nicotínico de acetilcolina, transportadores ABC, glicoproteína-P, proteínas afetadas por outros anti-helmínticos, proteínas de canal de íons, peptidases, kinases e genes da maquinaria epigenética de modificação de histonas. Em análises de enriquecimento, destacaram-se genes envolvidos em locomoção, atividade de peptidase, canal de íons, transportador e receptor acoplado à proteína-G. Ainda, por investigação in silico, foram encontradas evidências da ausência de metilação do DNA em H. contortus. Dessa maneira, os polimorfismos identificados precisam ser investigados como marcadores moleculares de resistência ao monepantel e, assim, contribuírem para o diagnóstico precoce da resistência e monitoramento da eficácia de anti-helmínticos. Além disso, podem ser considerados potenciais alvos para o desenvolvimento de tratamentos alternativos ou de novos fármacos, incluindo os de efeito epigenético, para o controle de H. contortus em rebanhos ovinos.
650 $aAnti-Helmíntico
650 $aGenoma
650 $aMarcador Molecular
650 $aMetilação
653 $aGastrenterite
653 $aIntrogressão de genes
653 $aMetilação de DNA
653 $aNematoide grastrintestinal de ovinos
653 $aResistência anti-helmíntica
653 $aSequenciamento genômico
700 1 $aMORAES, C. V.
700 1 $aCRUVINEL, G. G.
700 1 $aALEMÁN GAINZA, Y.
700 1 $aALBUQUERQUE, A. C. A. de
700 1 $aSANTANA, R. C. M.
700 1 $aTHOLON, P.
700 1 $aTIZIOTO, P. C.
700 1 $aCHAGAS, A. C. de S.
700 1 $aESTEVES, S. N.
700 1 $aBENAVIDES, M. V.
700 1 $aAMARANTE, A. F. T. do
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/09/2023 |
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Data da última atualização: |
11/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
AYMÉE, L.; DI AZEVEDO, M. I. N.; REIS, L.; MENDES, J.; CASTRO, F. de D. A. de; CARVALHO-COSTA, F. A. C.; SOUZA, G. N. de; LILENBAUM, W. |
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Afiliação: |
LUIZA AYMÉE, UNIVERSIDADE FEDERAL FLUMINENSE; MARIA ISABEL NOGUEIRA DI AZEVEDO, UNIVERSIDADE FEDERAL FLUMINENSE; LUIZA REIS, UNIVERSIDADE FEDERAL FLUMINENSE; JULIA MENDES, UNIVERSIDADE FEDERAL FLUMINENSE; FÚLVIA DE FÁTIMA ALMEIDA DE CASTRO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; FILIPE ANIBAL CARVALHO-COSTA, INSTITUTO OSWALDO CRUZ; GUILHERME NUNES DE SOUZA, CNPGL; WALTER LILENBAUM, UNIVERSIDADE FEDERAL FLUMINENSE. |
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Título: |
Unconventional sites for diagnosis of leptospirosis in bovine anicteric fetuses. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Animals, v. 13, 2832, 2023. |
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DOI: |
https://doi.org/10.3390/ani13182832 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Bovine leptospirosis is an important reproductive disease and abortion is a major sign, leading to economic impacts. Due to its multifactorial etiology, the proper diagnosis of the cause of the abortion is crucial. Necropsy of the fetuses followed by molecular analysis is recommended for diagnosis, and the investigation mainly occurs in the kidneys and liver. This study aimed to analyze unconventional sites for the presence of leptospiral DNA in bovine anicteric aborted fetuses. Methods: Five fetuses of the same herd were received for necropsy and diagnosis. Conventional lipL32-PCR was performed in the fetuses? kidneys, livers, lungs, hearts, spleens, subcapsular kidney content, abomasal fluid, and in the cavity?s hemorrhagic contents. To complete the investigation, the sera of 30 cows of the herd were collected to perform the serologic screening by Microscopic Agglutination Test. In addition, six subfertile non-pregnant cows from the same herd were selected due to their low reproductive performance, and genital samples (uterine fragment and cervicovaginal mucus) and urine were collected for lipL32-PCR. PCR-positive samples were submitted to a nested PCR of the secY gene and intended for sequencing. Results: The herd presented seroreactive animals (11/30, 36.6%), all against the Sejroe serogroup, with titers between 200 and 1600. In necropsy, four fetuses showed hemorrhagic and anicteric lesions, while one fetus had no macroscopic lesions. Regarding molecular analysis, all the fetuses were positive in lipL32-PCR and the positive sites were the heart, lungs, subcapsular kidney content, thymus, kidneys, liver, and abomasal fluid. Only one fetus presented positive results in the kidney and liver, while three fetuses were positive in the abomasal fluid. Five of six cows were positive for lipL32-PCR, all being positive only in genital samples. Of the fetuses and the cows, seven sequences were obtained and all were identified as Leptospira interrogans serogroup Sejroe serovar Hardjoprajitno. Conclusions: In order to improve the diagnosis of leptospirosis in cows, it is recommended to perform a comprehensive analysis of the samples, beyond the kidneys and liver. Thus, we highly encourage testing multiple organs by PCR to investigate abortions suspected of bovine leptospirosis, particularly in anicteric fetuses. MenosBackground: Bovine leptospirosis is an important reproductive disease and abortion is a major sign, leading to economic impacts. Due to its multifactorial etiology, the proper diagnosis of the cause of the abortion is crucial. Necropsy of the fetuses followed by molecular analysis is recommended for diagnosis, and the investigation mainly occurs in the kidneys and liver. This study aimed to analyze unconventional sites for the presence of leptospiral DNA in bovine anicteric aborted fetuses. Methods: Five fetuses of the same herd were received for necropsy and diagnosis. Conventional lipL32-PCR was performed in the fetuses? kidneys, livers, lungs, hearts, spleens, subcapsular kidney content, abomasal fluid, and in the cavity?s hemorrhagic contents. To complete the investigation, the sera of 30 cows of the herd were collected to perform the serologic screening by Microscopic Agglutination Test. In addition, six subfertile non-pregnant cows from the same herd were selected due to their low reproductive performance, and genital samples (uterine fragment and cervicovaginal mucus) and urine were collected for lipL32-PCR. PCR-positive samples were submitted to a nested PCR of the secY gene and intended for sequencing. Results: The herd presented seroreactive animals (11/30, 36.6%), all against the Sejroe serogroup, with titers between 200 and 1600. In necropsy, four fetuses showed hemorrhagic and anicteric lesions, while one fetus had no macroscopic lesions. Regarding molecular ana... Mostrar Tudo |
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Palavras-Chave: |
Abomasal fluid; Leptospiral infection; LipL32; Molecular analysis. |
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Thesagro: |
Aborto; Bovino; Gado; Leptospirose. |
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Thesaurus NAL: |
Abortion (animals); Cattle; Necropsy. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156556/1/Unconventional-sites-for-diagnosis-of-Leptospirosis-in-bovine-anicteric-fetuses.pdf
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Marc: |
LEADER 03302naa a2200349 a 4500
001 2156556
005 2023-09-11
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/ani13182832$2DOI
100 1 $aAYMÉE, L.
245 $aUnconventional sites for diagnosis of leptospirosis in bovine anicteric fetuses.$h[electronic resource]
260 $c2023
520 $aBackground: Bovine leptospirosis is an important reproductive disease and abortion is a major sign, leading to economic impacts. Due to its multifactorial etiology, the proper diagnosis of the cause of the abortion is crucial. Necropsy of the fetuses followed by molecular analysis is recommended for diagnosis, and the investigation mainly occurs in the kidneys and liver. This study aimed to analyze unconventional sites for the presence of leptospiral DNA in bovine anicteric aborted fetuses. Methods: Five fetuses of the same herd were received for necropsy and diagnosis. Conventional lipL32-PCR was performed in the fetuses? kidneys, livers, lungs, hearts, spleens, subcapsular kidney content, abomasal fluid, and in the cavity?s hemorrhagic contents. To complete the investigation, the sera of 30 cows of the herd were collected to perform the serologic screening by Microscopic Agglutination Test. In addition, six subfertile non-pregnant cows from the same herd were selected due to their low reproductive performance, and genital samples (uterine fragment and cervicovaginal mucus) and urine were collected for lipL32-PCR. PCR-positive samples were submitted to a nested PCR of the secY gene and intended for sequencing. Results: The herd presented seroreactive animals (11/30, 36.6%), all against the Sejroe serogroup, with titers between 200 and 1600. In necropsy, four fetuses showed hemorrhagic and anicteric lesions, while one fetus had no macroscopic lesions. Regarding molecular analysis, all the fetuses were positive in lipL32-PCR and the positive sites were the heart, lungs, subcapsular kidney content, thymus, kidneys, liver, and abomasal fluid. Only one fetus presented positive results in the kidney and liver, while three fetuses were positive in the abomasal fluid. Five of six cows were positive for lipL32-PCR, all being positive only in genital samples. Of the fetuses and the cows, seven sequences were obtained and all were identified as Leptospira interrogans serogroup Sejroe serovar Hardjoprajitno. Conclusions: In order to improve the diagnosis of leptospirosis in cows, it is recommended to perform a comprehensive analysis of the samples, beyond the kidneys and liver. Thus, we highly encourage testing multiple organs by PCR to investigate abortions suspected of bovine leptospirosis, particularly in anicteric fetuses.
650 $aAbortion (animals)
650 $aCattle
650 $aNecropsy
650 $aAborto
650 $aBovino
650 $aGado
650 $aLeptospirose
653 $aAbomasal fluid
653 $aLeptospiral infection
653 $aLipL32
653 $aMolecular analysis
700 1 $aDI AZEVEDO, M. I. N.
700 1 $aREIS, L.
700 1 $aMENDES, J.
700 1 $aCASTRO, F. de D. A. de
700 1 $aCARVALHO-COSTA, F. A. C.
700 1 $aSOUZA, G. N. de
700 1 $aLILENBAUM, W.
773 $tAnimals$gv. 13, 2832, 2023.
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