| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
17/01/2017 |
|
Data da última atualização: |
20/05/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SANTOS, L. F. dos; SILVA, R. J. S.; AMARAL, D. O. J. do; PAULA, M. F. B. de; FALCÃO, L. L.; LEGAVRE, T.; ALVES, R. M.; MARCELLINO, L. H.; MICHELI, F. |
|
Afiliação: |
Lucas Ferraz dos Santos, Universidade Estadual de Santa Cruz; Raner José Santana Silva, Universidade Estadual de Santa Cruz; Daniel Oliveira Jordão do Amaral, Universidade Estadual de Santa Cruz; Márcia Fabiana Barbosa de Paula, Universidade Estadual de Santa Cruz; LOENI LUDKE FALCAO, Cenargen; Thierry Legavre, CIRAD; RAFAEL MOYSES ALVES, CPATU; LUCILIA HELENA MARCELLINO, Cenargen; Fabienne Micheli, Universidade Estadual de Santa Cruz / CIRAD. |
|
Título: |
Selection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum. |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
Plos One, v. 11, n. 8, e0160646, 2016. |
|
DOI: |
http://dx.doi.org/10.1371/journal.pone.0160646 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Cupuassu (Theobroma grandiflorum [Willd. ex Spreng.] Schum) is a species of high economic importance in Brazil with great potential at international level due to the multiple uses of both its seeds and pulp in the industry of sweets and cosmetics. For this reason, the cupuassu breeding program focused on the selection of genotypes with high pulp and seed quality?selection associated with the understanding of the mechanisms involved in fruit formation. Gene expression is one of the most used approaches related to such understanding. In this sense, quantitative real-time PCR (qPCR) is a powerful tool, since it rapidly and reliably quantifies gene expression levels across different experimental conditions. The analysis by qPCR and the correct interpretation of data depend on signal normalization using reference genes, i.e. genes presenting a uniform pattern of expression in the analyzed samples. Here, we selected and analyzed the expression of five genes from cupuassu (ACP, ACT, GAPDH, MDH, TUB) to be used as candidates for reference genes on pulp and seed of young, maturing and mature cupuassu fruits. The evaluation of the gene expression stability was obtained using the NormFinder, geNorm and BestKeeper programs. In general, our results indicated that the GAPDH and MDH genes constituted the best combination as reference genes to analyze the expression of cupuassu samples. To our knowledge, this is the first report of reference gene definition in cupuassu, and these results will support subsequent analysis related to gene expression studies in cupuassu plants subjected to different biotic or abiotic conditions as well as serve as a tool for diversity analysis based on pulp and seed quality. MenosCupuassu (Theobroma grandiflorum [Willd. ex Spreng.] Schum) is a species of high economic importance in Brazil with great potential at international level due to the multiple uses of both its seeds and pulp in the industry of sweets and cosmetics. For this reason, the cupuassu breeding program focused on the selection of genotypes with high pulp and seed quality?selection associated with the understanding of the mechanisms involved in fruit formation. Gene expression is one of the most used approaches related to such understanding. In this sense, quantitative real-time PCR (qPCR) is a powerful tool, since it rapidly and reliably quantifies gene expression levels across different experimental conditions. The analysis by qPCR and the correct interpretation of data depend on signal normalization using reference genes, i.e. genes presenting a uniform pattern of expression in the analyzed samples. Here, we selected and analyzed the expression of five genes from cupuassu (ACP, ACT, GAPDH, MDH, TUB) to be used as candidates for reference genes on pulp and seed of young, maturing and mature cupuassu fruits. The evaluation of the gene expression stability was obtained using the NormFinder, geNorm and BestKeeper programs. In general, our results indicated that the GAPDH and MDH genes constituted the best combination as reference genes to analyze the expression of cupuassu samples. To our knowledge, this is the first report of reference gene definition in cupuassu, and these results wi... Mostrar Tudo |
|
Palavras-Chave: |
Genotipos. |
|
Thesagro: |
Cupuaçu. |
|
Categoria do assunto: |
--
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/153764/1/document-4.pdf
|
|
Marc: |
LEADER 02498naa a2200253 a 4500
001 2060964
005 2022-05-20
008 2016 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1371/journal.pone.0160646$2DOI
100 1 $aSANTOS, L. F. dos
245 $aSelection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum.$h[electronic resource]
260 $c2016
520 $aCupuassu (Theobroma grandiflorum [Willd. ex Spreng.] Schum) is a species of high economic importance in Brazil with great potential at international level due to the multiple uses of both its seeds and pulp in the industry of sweets and cosmetics. For this reason, the cupuassu breeding program focused on the selection of genotypes with high pulp and seed quality?selection associated with the understanding of the mechanisms involved in fruit formation. Gene expression is one of the most used approaches related to such understanding. In this sense, quantitative real-time PCR (qPCR) is a powerful tool, since it rapidly and reliably quantifies gene expression levels across different experimental conditions. The analysis by qPCR and the correct interpretation of data depend on signal normalization using reference genes, i.e. genes presenting a uniform pattern of expression in the analyzed samples. Here, we selected and analyzed the expression of five genes from cupuassu (ACP, ACT, GAPDH, MDH, TUB) to be used as candidates for reference genes on pulp and seed of young, maturing and mature cupuassu fruits. The evaluation of the gene expression stability was obtained using the NormFinder, geNorm and BestKeeper programs. In general, our results indicated that the GAPDH and MDH genes constituted the best combination as reference genes to analyze the expression of cupuassu samples. To our knowledge, this is the first report of reference gene definition in cupuassu, and these results will support subsequent analysis related to gene expression studies in cupuassu plants subjected to different biotic or abiotic conditions as well as serve as a tool for diversity analysis based on pulp and seed quality.
650 $aCupuaçu
653 $aGenotipos
700 1 $aSILVA, R. J. S.
700 1 $aAMARAL, D. O. J. do
700 1 $aPAULA, M. F. B. de
700 1 $aFALCÃO, L. L.
700 1 $aLEGAVRE, T.
700 1 $aALVES, R. M.
700 1 $aMARCELLINO, L. H.
700 1 $aMICHELI, F.
773 $tPlos One$gv. 11, n. 8, e0160646, 2016.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Amazônia Oriental (CPATU) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 4 | |
| 1. |  | ROSA. M. C. DA; SANTOS, F. D. S.; CONRAD, N. L.; PIRAINE, R. E. A.; GONÇALVES, V. S.; CUNHA, R. C.; ANDREOTTI, R. Cloning and expression of a chimera containing ROP2 of Neospora caninum fused with OprI lipoprotein from Pseudomonas aeruginosa. Brazilian Journal of Development, Curitiba, v.7, n.5, may. 2021. p. 44649-44659| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Gado de Corte. |
|    |
| 2. | | SANTOS JUNIOR, A. G. dos; PIRAINE, R. E. A.; CUNHA, R. C.; NIZOLI, L. Q.; ANDREOTTI, R.; LEITE, F. P. L. Avaliação de métodos para obtenção de proteínas recombinantes. Science and Animal Health, v.5, n.2, p. 166-177, maio/ago. 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 3. | | SILVA, R. A. e; PIRAINE, R. E. A.; SANTOS JUNIOR, A. G. dos; CUNHA, R. C.; GONÇALVES, V. S.; SANTOS, F. D. S.; LEITE, F. P. L. Situação atual das estratégias para desenvolvimento de antígenos para o controle da neosporose em bovinos. Brasília, DF: Embrapa, 2017. 48 p. (Embrapa Gado de Corte. Documentos, 229). Na publicação o nove do autor Renato Andreotti e Silva está como Renato Andreotti| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 4. | | ANDREOTTI, R.; CSORDAS, B. G.; CUNHA, R. C.; PIRAINE, R. E. A.; SANTOS, F. D. S.; GRASSMANN, A. A.; BARROS, J. C.; FREITAS, B. P. F.; MENEGON, Y. A.; LEITE, F. L. Composição do meio de cultura afeta produção de proteína recombinante de carrapato rhipicephalus microplus expressa em escherichia coli / composition of the medium culture affects recombinant protein production of the rhipicephalus microplus expressed in escherichia coli. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 19; NOVEL APPROACHES TO THE CONTROL OF HELMINTH PARASITES OF LIVESTOCK, 8, 2016, Belém, PA. Anais... Jaboticabal: CBPV, 2016| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
| |
| Registros recuperados : 4 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
