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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
24/12/2008 |
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Data da última atualização: |
15/01/2009 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA-PINHATI, A. C.; FREITAS-ÁSTUA, J.; AMARAL, A. M. do; MACHADO, M. A. |
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Afiliação: |
A. C. Silva-Pinhati, CCSM/IAC; Juliana Freitas-Ástua, CNPMF/CCSM/IAC; A. M. do Amaral, CCSM/IAC; M. A. Machado, CCSM/IAC. |
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Título: |
Expression of genes associate to biosynthesis of flavonoids of Citrus sinensis. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan, China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 161. P 132. |
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Idioma: |
Inglês |
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Conteúdo: |
Flavonoids constitute a major group of secondary metabolites and they are directly associated with citrus fruit quality. In plants, these compounds are involved in the protection against the biotic and abiotic stresses, cell structure, UV protection, attraction of pollinators and seed dispersal. Flavones represent one of the most abundant and important classes of flavonoids, occurring in almost all plant tissues. The formation of flavones is catalyzed by flavone synthase I or flavone synthase II (FNSII). One of the main enzymes in the flavonol biosynthesis pathway is the flavanone-3-hydroxylase (F3H). In a previous study, we identified putative novel genes involved in the synthesis of flavones and flavonols in Citrus sinensis through expressed sequence tags (ESTs) of the CitEST database. Even though flavones have been found in several species and varieties within the citrus genus, this is the first study to report the presence of two copies of the fnsII gene in sweet orange. Moreover, in addition to the available Citf3h gene sequence, we identified another f3h transcript in C. sinensis. Because of the importance of having been found putative novel copies of genes involved in flavonoids biosynthesis, here we report the characterization of such gene expression patterns in different tissues and citrus fruit developmental stages, studied by real time quantitative PCR for the validation of results observed in silico analysis. Some of these genes or copies of genes should be used for genetic transformation of citrus to improve the fruit quality. MenosFlavonoids constitute a major group of secondary metabolites and they are directly associated with citrus fruit quality. In plants, these compounds are involved in the protection against the biotic and abiotic stresses, cell structure, UV protection, attraction of pollinators and seed dispersal. Flavones represent one of the most abundant and important classes of flavonoids, occurring in almost all plant tissues. The formation of flavones is catalyzed by flavone synthase I or flavone synthase II (FNSII). One of the main enzymes in the flavonol biosynthesis pathway is the flavanone-3-hydroxylase (F3H). In a previous study, we identified putative novel genes involved in the synthesis of flavones and flavonols in Citrus sinensis through expressed sequence tags (ESTs) of the CitEST database. Even though flavones have been found in several species and varieties within the citrus genus, this is the first study to report the presence of two copies of the fnsII gene in sweet orange. Moreover, in addition to the available Citf3h gene sequence, we identified another f3h transcript in C. sinensis. Because of the importance of having been found putative novel copies of genes involved in flavonoids biosynthesis, here we report the characterization of such gene expression patterns in different tissues and citrus fruit developmental stages, studied by real time quantitative PCR for the validation of results observed in silico analysis. Some of these genes or copies of genes should be used... Mostrar Tudo |
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Palavras-Chave: |
Citros; Melhoramento genetico. |
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Thesagro: |
Genética Vegetal. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02225naa a2200193 a 4500
001 1655358
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100 1 $aSILVA-PINHATI, A. C.
245 $aExpression of genes associate to biosynthesis of flavonoids of Citrus sinensis.
260 $c2008
520 $aFlavonoids constitute a major group of secondary metabolites and they are directly associated with citrus fruit quality. In plants, these compounds are involved in the protection against the biotic and abiotic stresses, cell structure, UV protection, attraction of pollinators and seed dispersal. Flavones represent one of the most abundant and important classes of flavonoids, occurring in almost all plant tissues. The formation of flavones is catalyzed by flavone synthase I or flavone synthase II (FNSII). One of the main enzymes in the flavonol biosynthesis pathway is the flavanone-3-hydroxylase (F3H). In a previous study, we identified putative novel genes involved in the synthesis of flavones and flavonols in Citrus sinensis through expressed sequence tags (ESTs) of the CitEST database. Even though flavones have been found in several species and varieties within the citrus genus, this is the first study to report the presence of two copies of the fnsII gene in sweet orange. Moreover, in addition to the available Citf3h gene sequence, we identified another f3h transcript in C. sinensis. Because of the importance of having been found putative novel copies of genes involved in flavonoids biosynthesis, here we report the characterization of such gene expression patterns in different tissues and citrus fruit developmental stages, studied by real time quantitative PCR for the validation of results observed in silico analysis. Some of these genes or copies of genes should be used for genetic transformation of citrus to improve the fruit quality.
650 $aGenética Vegetal
653 $aCitros
653 $aMelhoramento genetico
700 1 $aFREITAS-ÁSTUA, J.
700 1 $aAMARAL, A. M. do
700 1 $aMACHADO, M. A.
773 $tIn: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan, China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 161. P 132.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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