Data/hora: 26/05/2024 21:56:25

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	24/12/2008	
Data da última atualização: 	15/01/2009	
Autoria: 	SILVA-PINHATI, A. C.; FREITAS-ÁSTUA, J.; AMARAL, A. M. do; MACHADO, M. A.	
Afiliação: 	A. C. Silva-Pinhati, CCSM/IAC; Juliana Freitas-Ástua, CNPMF/CCSM/IAC; A. M. do Amaral, CCSM/IAC; M. A. Machado, CCSM/IAC.	
Título: 	Expression of genes associate to biosynthesis of flavonoids of Citrus sinensis.	
Ano de publicação: 	2008	
Fonte/Imprenta: 	In: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan, China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 161. P 132.	
Idioma: 	Inglês	
Conteúdo: 	Flavonoids constitute a major group of secondary metabolites and they are directly  associated with citrus fruit quality. In plants, these compounds are involved in the protection against the biotic and abiotic stresses, cell structure, UV protection, attraction of pollinators and seed dispersal. Flavones represent one of the most abundant and important classes of flavonoids, occurring in almost all plant tissues. The formation of flavones is catalyzed by flavone synthase I or flavone synthase II (FNSII). One of the main enzymes in the flavonol biosynthesis pathway is the flavanone-3-hydroxylase (F3H). In a previous study, we identified putative novel genes involved in the synthesis of flavones and flavonols in Citrus sinensis through expressed sequence tags (ESTs) of the CitEST database. Even though flavones have been found in several species and varieties within the citrus genus, this is the first study to report the presence of two copies of the fnsII gene in sweet orange. Moreover, in addition to the available Citf3h gene sequence, we identified another f3h transcript in C. sinensis. Because of the importance of having been found putative novel copies of genes involved in flavonoids biosynthesis, here we report the characterization of such gene expression patterns in different tissues and citrus fruit developmental stages, studied by real time quantitative PCR for the validation of results observed in silico analysis. Some of these genes or copies of genes should be used for genetic transformation of citrus to improve the fruit quality.	
Palavras-Chave: 	Citros; Melhoramento genetico.	
Thesagro: 	Genética Vegetal.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)