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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
24/02/2016 |
Data da última atualização: |
03/04/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LOPES, T. A.; COSTA, J. N.; RIBEIRO, R. P.; PASSOS, J. R. S.; SOARES, M. A. A.; ALVES FILHO, J. G.; CUNHA, E. V.; HURK, R. van den; ANDRIOLI, A.; SILVA, J. R. V. |
Afiliação: |
Biotechnology Nucleus of Sobral (NUBIS) - Federal University of Ceara (UFC) - Sobral, CE, Brazil; Biotechnology Nucleus of Sobral (NUBIS), Federal University of Ceara (UFC) - Sobral, CE, Brazil; Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University - Utrecht, The Netherlands; ALICE ANDRIOLI, CNPC. |
Título: |
Influence of caprine arthritis encephalitis on expression ofovulation related genes and activation of primordial folliclescultured in presence of phytohemagglutinin, epidermalgrowth factor or bothT. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Small Ruminant Research, Amsterdam, v. 123, n. 2/3, p. 278-286, Feb. 2015. |
DOI: |
10.1016/j.smallrumres.2014.11.016 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: This study investigated the levels of mRNA for BMP15, GDF9 and BMPR1B in preantral follicles, as well as in cumulus oocyte complex (COC) and follicular walls from antral follicles of healthy and CAEV infected goats by real-time PCR. In addition, the influence of phytohemagglutinin (PHA) and EGF on activation of primordial follicles and on expression of mRNA for TNFa and its receptors in cultured ovarian tissue from healthy and infected goats was evaluated. The expression of BMP15 and GDF9 in primordial/primary follicles from infected goats was significantly higher than in healthy animals, whereas the expression of BMP15 and GDF9 in secondary follicles from infected goats was significantly lower. The expression of mRNA for BMP15 in follicular wall of antral follicles from infected goats was significantly higher than in healthy goats. CAEV infection did not influence the ability of primordial follicles to develop in vitro, but the presence of EGF in culture medium significantly increased follicular diameter in healthy and infected animals. Ovarian tissue from infected goats cultured in medium with PHA had primordial follicles with larger diameters than those from healthy animals. An increase of mRNAs for TNF? was observed after culturing tissue in presence of both EGF and PHA in healthy animals, but this same treatment promoted a reduction of mRNAs for TNFa and increase of TNFR2 transcripts in infected animals. In conclusion, CAEV influences the expression of mRNA for BMP15 and GDF9 in goat ovarian follicles and PHA and EGF differentially regulate the expression of TNFa and TNFR2 in cultured ovarian tissue. MenosAbstract: This study investigated the levels of mRNA for BMP15, GDF9 and BMPR1B in preantral follicles, as well as in cumulus oocyte complex (COC) and follicular walls from antral follicles of healthy and CAEV infected goats by real-time PCR. In addition, the influence of phytohemagglutinin (PHA) and EGF on activation of primordial follicles and on expression of mRNA for TNFa and its receptors in cultured ovarian tissue from healthy and infected goats was evaluated. The expression of BMP15 and GDF9 in primordial/primary follicles from infected goats was significantly higher than in healthy animals, whereas the expression of BMP15 and GDF9 in secondary follicles from infected goats was significantly lower. The expression of mRNA for BMP15 in follicular wall of antral follicles from infected goats was significantly higher than in healthy goats. CAEV infection did not influence the ability of primordial follicles to develop in vitro, but the presence of EGF in culture medium significantly increased follicular diameter in healthy and infected animals. Ovarian tissue from infected goats cultured in medium with PHA had primordial follicles with larger diameters than those from healthy animals. An increase of mRNAs for TNF? was observed after culturing tissue in presence of both EGF and PHA in healthy animals, but this same treatment promoted a reduction of mRNAs for TNFa and increase of TNFR2 transcripts in infected animals. In conclusion, CAEV influences the expression of mRNA fo... Mostrar Tudo |
Palavras-Chave: |
CAEV; Caprine arthritis encephalit virus; Fitohemaglutinina; Ovary; Phytohaemagglutinins; TNF. |
Thesagro: |
Cabra; Caprino; Ovário. |
Thesaurus Nal: |
Goats. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02776naa a2200361 a 4500 001 2038345 005 2019-04-03 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1016/j.smallrumres.2014.11.016$2DOI 100 1 $aLOPES, T. A. 245 $aInfluence of caprine arthritis encephalitis on expression ofovulation related genes and activation of primordial folliclescultured in presence of phytohemagglutinin, epidermalgrowth factor or bothT.$h[electronic resource] 260 $c2015 520 $aAbstract: This study investigated the levels of mRNA for BMP15, GDF9 and BMPR1B in preantral follicles, as well as in cumulus oocyte complex (COC) and follicular walls from antral follicles of healthy and CAEV infected goats by real-time PCR. In addition, the influence of phytohemagglutinin (PHA) and EGF on activation of primordial follicles and on expression of mRNA for TNFa and its receptors in cultured ovarian tissue from healthy and infected goats was evaluated. The expression of BMP15 and GDF9 in primordial/primary follicles from infected goats was significantly higher than in healthy animals, whereas the expression of BMP15 and GDF9 in secondary follicles from infected goats was significantly lower. The expression of mRNA for BMP15 in follicular wall of antral follicles from infected goats was significantly higher than in healthy goats. CAEV infection did not influence the ability of primordial follicles to develop in vitro, but the presence of EGF in culture medium significantly increased follicular diameter in healthy and infected animals. Ovarian tissue from infected goats cultured in medium with PHA had primordial follicles with larger diameters than those from healthy animals. An increase of mRNAs for TNF? was observed after culturing tissue in presence of both EGF and PHA in healthy animals, but this same treatment promoted a reduction of mRNAs for TNFa and increase of TNFR2 transcripts in infected animals. In conclusion, CAEV influences the expression of mRNA for BMP15 and GDF9 in goat ovarian follicles and PHA and EGF differentially regulate the expression of TNFa and TNFR2 in cultured ovarian tissue. 650 $aGoats 650 $aCabra 650 $aCaprino 650 $aOvário 653 $aCAEV 653 $aCaprine arthritis encephalit virus 653 $aFitohemaglutinina 653 $aOvary 653 $aPhytohaemagglutinins 653 $aTNF 700 1 $aCOSTA, J. N. 700 1 $aRIBEIRO, R. P. 700 1 $aPASSOS, J. R. S. 700 1 $aSOARES, M. A. A. 700 1 $aALVES FILHO, J. G. 700 1 $aCUNHA, E. V. 700 1 $aHURK, R. van den 700 1 $aANDRIOLI, A. 700 1 $aSILVA, J. R. V. 773 $tSmall Ruminant Research, Amsterdam$gv. 123, n. 2/3, p. 278-286, Feb. 2015.
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Embrapa Caprinos e Ovinos (CNPC) |
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Registros recuperados : 9 | |
3. | | ARAGÃO, M. A. do C.; PINHEIRO, R. R.; ALVES, F. S. F.; FONSECA, A. A. da; ALVES FILHO, J. G. Corynebacterium pseudotuberculosis isolado de abscesso na base do cérebro: relato de caso. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 5.; ENCONTRO DE PÓS-GRADUAÇÃO E PESQUISA, 2., 2003, Sobral. Anais... Sobral: Universidade Estadual Vale do Acaraú, 2003. p. 118.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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4. | | ALVES FILHO, J. G.; ALVES, F. S. F.; PINHEIRO, R. R.; FONSECA, A. A. da; ARAGÃO, M. A. do C. Onfaloflebite e artrite em caprinos causada por Corynebacterium pseudotuberculosis: relato de caso. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 5.; ENCONTRO DE PÓS GRADUAÇÃO E PESQUISA, 2., 2003, Sobral. Anais... Sobral: Universidade Estadual Vale do Acaraú, 2003. p. 139Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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6. | | LOPES, T. A.; COSTA, J. N.; RIBEIRO, R. P.; PASSOS, J. R. S.; SOARES, M. A. A.; ALVES FILHO, J. G.; CUNHA, E. V.; HURK, R. van den; ANDRIOLI, A.; SILVA, J. R. V. Influence of caprine arthritis encephalitis on expression ofovulation related genes and activation of primordial folliclescultured in presence of phytohemagglutinin, epidermalgrowth factor or bothT. Small Ruminant Research, Amsterdam, v. 123, n. 2/3, p. 278-286, Feb. 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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7. | | SOARES, F. S.; AMARAL, F. C.; SILVA, N. L. S.; VALENTE, M. R.; SANTOS, L. K. R.; YAMASHIRO, L. H.; SCHEFFE, M. S.; CASTANHEIRA, F. V. E. S.; FERREIRA, R. G.; GEHRKE, L.; ALVES-FILHO, J. C.; SILVA, L. P.; BÁFICA, A.; SPILLER, F. Antibiotic-Induced Pathobiont Dissemination Accelerates Mortality in Severe Experimental Pancreatitis. Frontiers in Immunology, v. 8, 2017. Article 1890.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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8. | | PINTO, T. M. F.; MOREIRA, R. F.; MATOS, M. N. C.; SOARES, V. V. M.; AGUIAR, M. V. de A.; ARAGÃO, P. de T. T. D. de; ALVES FILHO, J. G.; MORENO, F. B. M. B.; MONTEIRO-MOREIRA, A. C. de O.; COSTA, C. R. R.; LIMA FILHO, J. L. de; ELOY, A. M. X.; CUNHA, R. M. S. da. Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus). Animal Reproduction, v. 16, n. 4, p. 902-913, 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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9. | | MOREIRA, R. F.; MATOS, M. N. C.; ALVES FILHO, J. G.; VALLE, R. V. do; ELOY, A. M. X.; PINTO, T. M. F.; MACHADO, S. P.; COSTA, C. R. R.; LIMA FILHO, J. L. de; LIMA, J. P. M. S.; CUNHA, R. M. S. da. Diversity of ejaculated sperm proteins in Moxotó bucks (Capra hircus) evaluated by multiple extraction methods. Animal Reproduction, Belo Horizonte, v. 15, n. 1, p. 84-92, jan./mar. 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registros recuperados : 9 | |
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