| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Uva e Vinho. |
|
Data corrente: |
07/12/2018 |
|
Data da última atualização: |
19/12/2018 |
|
Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
|
Autoria: |
ALVES, S. A. M.; SANTOS, R. S. S. dos. |
|
Afiliação: |
SILVIO ANDRE MEIRELLES ALVES, CNPUV; REGIS SIVORI SILVA DOS SANTOS, CNPUV. |
|
Título: |
Método para monitoramento da atividade de insetos por meio de fotos. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Bento Gonçalves, RS: Embrapa Uva e vinho, 2018. |
|
Série: |
(Embrapa Uva e Vinho. Comunicado Técnico, 208) |
|
ISSN: |
1808-6802 |
|
Idioma: |
Português |
|
Conteúdo: |
Foi desenvolvido um método para monitoramento de atividade de insetos por meio de fotos em condições de campo por um período mínimo de 12h. O método proposto utiliza máquina fotográfica da marca Canon, família "PowerShot"; um adaptador AC; uma atualização de firmware; uma caixa tipo ?estanque? para proteção da chuva, orvalho e poeira; e um cartão de memória compatível com a quantidade de fotos programada. O método pode ser ajustado de acordo com as necessidades específicas de registro, quanto ao intervalo entre as fotos, período inicial e final de registro. O limite do método ficará em função de quantas fotos poderão ser armazenadas no cartão de memória. Isso deverá ser planejado antes do início de registro das imagens e será em função do tamanho do cartão, da resolução de cada foto e do número total a ser adquirido. O que ela Resolve? A avaliação da visita ou comportamento de insetos a olho nu, e em tempo real, é muito trabalhosa e onerosa, pois se pode dispensar grande tempo sem nenhuma informação ter sido coletada. Para superar essas dificuldades foi desenvolvido o método que possibilita realizar as avaliações a posteriori, a partir de imagens digitais dos locais de observação. |
|
Palavras-Chave: |
Extremo Sul; Fotos; Monitoramento atividade de insetos; Vegetal. |
|
Thesagro: |
Controle Biológico; Fotografia; Inseto; Insumo. |
|
Categoria do assunto: |
O Insetos e Entomologia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/187983/1/Comunicado-Tecnico-208.pdf
|
|
Marc: |
LEADER 01949nam a2200241 a 4500
001 2100978
005 2018-12-19
008 2018 bl uuuu u0uu1 u #d
022 $a1808-6802
100 1 $aALVES, S. A. M.
245 $aMétodo para monitoramento da atividade de insetos por meio de fotos.$h[electronic resource]
260 $aBento Gonçalves, RS: Embrapa Uva e vinho$c2018
490 $a(Embrapa Uva e Vinho. Comunicado Técnico, 208)
520 $aFoi desenvolvido um método para monitoramento de atividade de insetos por meio de fotos em condições de campo por um período mínimo de 12h. O método proposto utiliza máquina fotográfica da marca Canon, família "PowerShot"; um adaptador AC; uma atualização de firmware; uma caixa tipo ?estanque? para proteção da chuva, orvalho e poeira; e um cartão de memória compatível com a quantidade de fotos programada. O método pode ser ajustado de acordo com as necessidades específicas de registro, quanto ao intervalo entre as fotos, período inicial e final de registro. O limite do método ficará em função de quantas fotos poderão ser armazenadas no cartão de memória. Isso deverá ser planejado antes do início de registro das imagens e será em função do tamanho do cartão, da resolução de cada foto e do número total a ser adquirido. O que ela Resolve? A avaliação da visita ou comportamento de insetos a olho nu, e em tempo real, é muito trabalhosa e onerosa, pois se pode dispensar grande tempo sem nenhuma informação ter sido coletada. Para superar essas dificuldades foi desenvolvido o método que possibilita realizar as avaliações a posteriori, a partir de imagens digitais dos locais de observação.
650 $aControle Biológico
650 $aFotografia
650 $aInseto
650 $aInsumo
653 $aExtremo Sul
653 $aFotos
653 $aMonitoramento atividade de insetos
653 $aVegetal
700 1 $aSANTOS, R. S. S. dos
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Uva e Vinho (CNPUV) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Solos. |
|
Data corrente: |
16/11/2018 |
|
Data da última atualização: |
11/11/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
ALESSI, A. M.; BIRD, S. M.; OATES, N. C.; LI, Y.; DOWLE, A. A.; NOVOTNY, E. H.; AZEVEDO, E. R. de; BENNETT, J. P.; POLIKARPOV, I.; YOUNG, J. P. W.; MCQUEEN-MASON, S. J.; BRUCE, N. C. |
|
Afiliação: |
ANNA M. ALESSI, UNIVERSITY OF YORK; SUSANNAH M. BIRD, UNIVERSITY OF YORK; NICOLA C. OATES, UNIVERSITY OF YORK; YI LI, UNIVERSITY OF YORK; ADAM A. DOWLE, UNIVERSITY OF YORK; ETELVINO HENRIQUE NOVOTNY, CNPS; EDUARDO R. DE AZEVEDO, USP; JOSEPH P. BENNETT, UNIVERSITY OF YORK; IGOR POLIKARPOV, USP; J. PETER W. YOUNG, UNIVERSITY OF YORK; SIMON J. MCQUEEN-MASON, UNIVERSITY OF YORK; NEIL C. BRUCE, UNIVERSITY OF YORK. |
|
Título: |
Defining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Biotechnology for Biofuels, v. 11, article 166, 2018. |
|
DOI: |
https://doi.org/10.1186/s13068-018-1164-2 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Background: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacaulis, Leadbetterella and Truepera. The eukaryotic members of the community were enriched in peritrich ciliates from genus Telotrochidium that thrived in the liquid cultures compared to fungal species that were present in low abundance. A targeted metasecretome approach combined with metatranscriptomics analysis, identified 1127 proteins and showed the presence of numerous carbohydrate-active enzymes extracted from the biomassbound fractions and from the culture supernatant. This revealed a wide array of hydrolytic cellulases, hemicellulases and carbohydrate-binding modules involved in lignocellulose degradation. The expression of these activities correlated to the changes in the biomass composition observed by FTIR and ssNMR measurements. Conclusions: A combination of mass spectrometry-based proteomics coupled with metatranscriptomics has enabled the identification of a large number of lignocellulose degrading enzymes that can now be further explored for the development of improved enzyme cocktails for the treatment of plant-based feedstocks. In addition to the expected carbohydrate-active enzymes, our studies reveal a large number of unknown proteins, some of which may play a crucial role in community-based lignocellulose degradation. MenosBackground: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacau... Mostrar Tudo |
|
Palavras-Chave: |
CAZy; Metasecretome. |
|
Thesaurus NAL: |
Lignocellulose. |
|
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/186133/1/2018-044.pdf
|
|
Marc: |
LEADER 03644naa a2200301 a 4500
001 2099501
005 2021-11-11
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s13068-018-1164-2$2DOI
100 1 $aALESSI, A. M.
245 $aDefining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies.$h[electronic resource]
260 $c2018
520 $aBackground: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacaulis, Leadbetterella and Truepera. The eukaryotic members of the community were enriched in peritrich ciliates from genus Telotrochidium that thrived in the liquid cultures compared to fungal species that were present in low abundance. A targeted metasecretome approach combined with metatranscriptomics analysis, identified 1127 proteins and showed the presence of numerous carbohydrate-active enzymes extracted from the biomassbound fractions and from the culture supernatant. This revealed a wide array of hydrolytic cellulases, hemicellulases and carbohydrate-binding modules involved in lignocellulose degradation. The expression of these activities correlated to the changes in the biomass composition observed by FTIR and ssNMR measurements. Conclusions: A combination of mass spectrometry-based proteomics coupled with metatranscriptomics has enabled the identification of a large number of lignocellulose degrading enzymes that can now be further explored for the development of improved enzyme cocktails for the treatment of plant-based feedstocks. In addition to the expected carbohydrate-active enzymes, our studies reveal a large number of unknown proteins, some of which may play a crucial role in community-based lignocellulose degradation.
650 $aLignocellulose
653 $aCAZy
653 $aMetasecretome
700 1 $aBIRD, S. M.
700 1 $aOATES, N. C.
700 1 $aLI, Y.
700 1 $aDOWLE, A. A.
700 1 $aNOVOTNY, E. H.
700 1 $aAZEVEDO, E. R. de
700 1 $aBENNETT, J. P.
700 1 $aPOLIKARPOV, I.
700 1 $aYOUNG, J. P. W.
700 1 $aMCQUEEN-MASON, S. J.
700 1 $aBRUCE, N. C.
773 $tBiotechnology for Biofuels$gv. 11, article 166, 2018.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Solos (CNPS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
