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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
15/08/2017 |
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Data da última atualização: |
17/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CASTAÑEDA, N. E. N.; ALVES, G. S. C.; ALMEIDA, R. M.; AMORIM, E. P.; FERREIRA, C. F.; TOGAWA, R. C.; COSTA, M. M. do C.; GRYNBERG, P.; SANTOS, J. R. P.; CARES, J. E.; MILLER, R. N. G. |
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Afiliação: |
NANCY EUNICE NIÑO CASTAÑEDA, UNB; GABRIEL SERGIO COSTA ALVES, UNB; ROSANE MANSAN ALMEIDA, UNB; EDSON PERITO AMORIM, CNPMF; CLAUDIA FORTES FERREIRA, CNPMF; ROBERTO COITI TOGAWA, CENARGEN; MARCOS MOTA DO CARMO COSTA, CENARGEN; PRISCILA GRYNBERG, Cenargen; JANSEN RODRIGO PEREIRA SANTOS, UNIVERSITY OF CALIFORNIA, USA; JUVENIL ENRIQUE CARES, UNB; ROBERT NEIL GERARD MILLER, UNB. |
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Título: |
Gene expression analysis in Musa acuminata during compatible interactions with Meloidogyne incognita. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Annals of Botany, v. 119, p. 915-930, 2017. |
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DOI: |
10.1093/aob/mcw272 |
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Idioma: |
Inglês |
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Conteúdo: |
Background and Aims: Endoparasitic root-knot nematodes (RKNs) ( Meloidogyne spp.) cause considerable losses in banana ( Musa spp.), with Meloidogyne incognita a predominant species in Cavendish sub-group bananas. This study investigates the root transcriptome in Musa acuminata genotypes 4297-06 (AA) and Cavendish Grande Naine (CAV; AAA) during early compatible interactions with M. incognita . Methods: Roots were analysed by brightfield light microscopy over a 35 d period to examine nematode penetration and morphological cell transformation. RNA samples were extracted 3, 7 and 10 days after inoculation (DAI) with nematode J2 juveniles, and cDNA libraries were sequenced using lllumina HiSeq technology. Sequences were mapped to the M. acuminata ssp. malaccensis var. Pahang genome sequence, differentially expressed genes (DEGs) identified and transcript representation determined by gene set enrichment and pathway mapping. Key Results: Microscopic analysis revealed a life cycle of M. incognita completing in 24 d in CAV and 27 d in 4279-06. Comparable numbers of DEGs were up- and downregulated in each genotype, with potential involvement of many in early host defence responses involving reactive oxygen species and jasmonate/ethylene signalling. DEGs revealed concomitant auxin metabolism and cell wall modification processes likely to be involved in giant cell formation. Notable transcripts related to host defence included those coding for leucine-rich repeat receptor-like serine/threonine-protein kinases, peroxidases, thaumatin-like pathogenesis-related proteins, and DREB, ERF, MYB, NAC and WRKY transcription factors. Transcripts related to giant cell development included indole acetic acid-amido synthetase GH3.8 genes, involved in auxin metabolism, as well as genes encoding expansins and hydrolases, involved in cell wall modification. Conclusions: Expression analysis in M. acuminata during compatible interactions with RKNs provides insights into genes modulated during infection and giant cell formation. Increased understanding of both defence responses to limit parasitism during compatible interactions and effector-targeted host genes in this complex interaction will facilitate the development of genetic improvement measures for RKNs. MenosBackground and Aims: Endoparasitic root-knot nematodes (RKNs) ( Meloidogyne spp.) cause considerable losses in banana ( Musa spp.), with Meloidogyne incognita a predominant species in Cavendish sub-group bananas. This study investigates the root transcriptome in Musa acuminata genotypes 4297-06 (AA) and Cavendish Grande Naine (CAV; AAA) during early compatible interactions with M. incognita . Methods: Roots were analysed by brightfield light microscopy over a 35 d period to examine nematode penetration and morphological cell transformation. RNA samples were extracted 3, 7 and 10 days after inoculation (DAI) with nematode J2 juveniles, and cDNA libraries were sequenced using lllumina HiSeq technology. Sequences were mapped to the M. acuminata ssp. malaccensis var. Pahang genome sequence, differentially expressed genes (DEGs) identified and transcript representation determined by gene set enrichment and pathway mapping. Key Results: Microscopic analysis revealed a life cycle of M. incognita completing in 24 d in CAV and 27 d in 4279-06. Comparable numbers of DEGs were up- and downregulated in each genotype, with potential involvement of many in early host defence responses involving reactive oxygen species and jasmonate/ethylene signalling. DEGs revealed concomitant auxin metabolism and cell wall modification processes likely to be involved in giant cell formation. Notable transcripts related to host defence included those coding for leucine-rich repeat receptor-like serine/th... Mostrar Tudo |
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Palavras-Chave: |
Monocotyledons; Root-knot nematode. |
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Thesagro: |
Meloidogyne Incognita; Musa Acuminata. |
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Thesaurus Nal: |
Biotic stress; Transcriptome. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166757/1/Gene-expression-analysis-in-Musa-Artigo-1.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162722/1/mcw272.pdf
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Marc: |
LEADER 03215naa a2200325 a 4500
001 2076414
005 2023-04-17
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1093/aob/mcw272$2DOI
100 1 $aCASTAÑEDA, N. E. N.
245 $aGene expression analysis in Musa acuminata during compatible interactions with Meloidogyne incognita.$h[electronic resource]
260 $c2017
520 $aBackground and Aims: Endoparasitic root-knot nematodes (RKNs) ( Meloidogyne spp.) cause considerable losses in banana ( Musa spp.), with Meloidogyne incognita a predominant species in Cavendish sub-group bananas. This study investigates the root transcriptome in Musa acuminata genotypes 4297-06 (AA) and Cavendish Grande Naine (CAV; AAA) during early compatible interactions with M. incognita . Methods: Roots were analysed by brightfield light microscopy over a 35 d period to examine nematode penetration and morphological cell transformation. RNA samples were extracted 3, 7 and 10 days after inoculation (DAI) with nematode J2 juveniles, and cDNA libraries were sequenced using lllumina HiSeq technology. Sequences were mapped to the M. acuminata ssp. malaccensis var. Pahang genome sequence, differentially expressed genes (DEGs) identified and transcript representation determined by gene set enrichment and pathway mapping. Key Results: Microscopic analysis revealed a life cycle of M. incognita completing in 24 d in CAV and 27 d in 4279-06. Comparable numbers of DEGs were up- and downregulated in each genotype, with potential involvement of many in early host defence responses involving reactive oxygen species and jasmonate/ethylene signalling. DEGs revealed concomitant auxin metabolism and cell wall modification processes likely to be involved in giant cell formation. Notable transcripts related to host defence included those coding for leucine-rich repeat receptor-like serine/threonine-protein kinases, peroxidases, thaumatin-like pathogenesis-related proteins, and DREB, ERF, MYB, NAC and WRKY transcription factors. Transcripts related to giant cell development included indole acetic acid-amido synthetase GH3.8 genes, involved in auxin metabolism, as well as genes encoding expansins and hydrolases, involved in cell wall modification. Conclusions: Expression analysis in M. acuminata during compatible interactions with RKNs provides insights into genes modulated during infection and giant cell formation. Increased understanding of both defence responses to limit parasitism during compatible interactions and effector-targeted host genes in this complex interaction will facilitate the development of genetic improvement measures for RKNs.
650 $aBiotic stress
650 $aTranscriptome
650 $aMeloidogyne Incognita
650 $aMusa Acuminata
653 $aMonocotyledons
653 $aRoot-knot nematode
700 1 $aALVES, G. S. C.
700 1 $aALMEIDA, R. M.
700 1 $aAMORIM, E. P.
700 1 $aFERREIRA, C. F.
700 1 $aTOGAWA, R. C.
700 1 $aCOSTA, M. M. do C.
700 1 $aGRYNBERG, P.
700 1 $aSANTOS, J. R. P.
700 1 $aCARES, J. E.
700 1 $aMILLER, R. N. G.
773 $tAnnals of Botany$gv. 119, p. 915-930, 2017.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| 3. |  | COUTINHO, A. C. Dinâmica das queimadas no Mato Grosso. In: C0NFERÊNCIA CIENTÍFICA DO LBA, 3., 2004, Brasília, DF. Anais: Experimentos de Grande Escala da Biosfera-Atmosfera na Amazônia. Brasília: ECO-LBA, 2004. 1 CD-ROM| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Territorial. |
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| 14. | | MATTO, C.; MIRANDA, J. R.; ZONTA, M.; COUTINHO, A. C. Cartografia do uso atual das terras nas Áreas de Proteção Ambiental de Sousas e Joaquim Egídio, Campinas-SP, Brasil. In: SIMPÓSIO BRASILEIRO DE SENSORIAMENTO REMOTO, 8., 1996, Salvador. Anais... São José dos Campos: INPE, 1996; Equador: SELPER, 1996. 2 p. folhas avulsas| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Territorial. |
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| 17. | | GUIMARÃES, M.; COUTINHO, A. C.; DORADO, A. J. Monitoramento da cobertura e dos usos do solo em uma região do Mato Grosso, Brasil, através da imagens TM-LANDSAT. In: SIMPÓSIO LATINOAMERICANO DE PERCEPCIÓN REMOTA, 9., 2000, Puerto Iquazú, Misiones-Argentina. Resumenes... Argentina: SELPER, 2000. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Territorial. |
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| Registros recuperados : 129 | |
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