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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
18/12/2017 |
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Data da última atualização: |
18/06/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HE, Y.; MATHIEU, J.; YANG, Y.; YU, P.; SILVA, M. L. B. da; ALVAREZ, P. J. J. |
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Afiliação: |
YA HE, Rice University Houston - Texas; JACQUES MATHIEU, Rice University Houston - Texas; YU YANG, Rice University Houston - Texas; PINGFENG YU, Rice University Houston - Texas; MARCIO LUIS BUSI DA SILVA, CNPSA; PEDRO J. J. ALVAREZ, Rice University Houston - Texas. |
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Título: |
Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Environmental Science & Technology, v. 4, n. 11, p. 494-499, 2017. |
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DOI: |
10.1021/acs.estlett.7b00456 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites and minimize false negatives. MenosABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites ... Mostrar Tudo |
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Palavras-Chave: |
Biodegradação de dioxano; Microbactéria. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198674/1/marcio-busi.pdf
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Marc: |
LEADER 02224naa a2200217 a 4500
001 2082755
005 2019-06-18
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1021/acs.estlett.7b00456$2DOI
100 1 $aHE, Y.
245 $aDioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase.$h[electronic resource]
260 $c2017
520 $aABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites and minimize false negatives.
653 $aBiodegradação de dioxano
653 $aMicrobactéria
700 1 $aMATHIEU, J.
700 1 $aYANG, Y.
700 1 $aYU, P.
700 1 $aSILVA, M. L. B. da
700 1 $aALVAREZ, P. J. J.
773 $tEnvironmental Science & Technology$gv. 4, n. 11, p. 494-499, 2017.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
25/10/2012 |
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Data da última atualização: |
25/10/2012 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SANTOS, V. V. dos; HOLTZ, A. M.; BOTTI, J. M. C.; PAULO, H. H. de; FRANZIN, M. L.; PRATISSOLI, D.; MACHADO, L. C.; RONDELLI, V. M.; PIRES, A. A. |
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Afiliação: |
Vanessa Victer dos Santos, Graduanda do curso de LICA do IFES; Anderson Mathias Holtz, Doutor em Entomologia Agrícola, Professor do IFES; Jéssica Mayara Coffler Botti, Graduanda do curso de Agronomia do IFES; Hágabo Honorato de Paulo, Doutor em Entomologia Agrícola, Professor do IFES; Mayara Loss Franzin, Doutora em Entomologia Agrícola, Professor do IFES; Dirceu Pratissoli, Doutor em Entomologia Agrícola, professor da UFES; Lorena Contarini Machado, Graduanda do curso de Agronomia da UFES; Vando Miossi Rondelli, Doutorando em Entomologia Agrícola da UFRPE; André Assis Pires, Doutor em Produção Vegetal, professor do IFES. |
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Título: |
O extrato da casca do caule de JATROPHA CURCAS pode ser utilizando no controle de Planococcus citri? |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO-MANSO, 1., 2012, Guarapari. Desafios e Oportunidades: anais. Campina Grande: Embrapa Algodão, 2012. |
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Páginas: |
p. 218 |
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Idioma: |
Português |
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Palavras-Chave: |
COCHONILHA DA ROSETA; CONTROLE ALTERNATIVO; FITOSSANIDADE; JATROPHA CURCAS L; PINHÃO MANSO. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/68805/1/FIT-004-P.233.pdf
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Marc: |
LEADER 00951nam a2200265 a 4500
001 1938114
005 2012-10-25
008 2012 bl uuuu u00u1 u #d
100 1 $aSANTOS, V. V. dos
245 $aO extrato da casca do caule de JATROPHA CURCAS pode ser utilizando no controle de Planococcus citri?
260 $aIn: CONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO-MANSO, 1., 2012, Guarapari. Desafios e Oportunidades: anais. Campina Grande: Embrapa Algodão$c2012
300 $ap. 218
653 $aCOCHONILHA DA ROSETA
653 $aCONTROLE ALTERNATIVO
653 $aFITOSSANIDADE
653 $aJATROPHA CURCAS L
653 $aPINHÃO MANSO
700 1 $aHOLTZ, A. M.
700 1 $aBOTTI, J. M. C.
700 1 $aPAULO, H. H. de
700 1 $aFRANZIN, M. L.
700 1 $aPRATISSOLI, D.
700 1 $aMACHADO, L. C.
700 1 $aRONDELLI, V. M.
700 1 $aPIRES, A. A.
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Embrapa Algodão (CNPA) |
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