Data/hora: 03/06/2024 08:30:09

Biblioteca(s): | Embrapa Cerrados.
Data corrente: | 26/03/2012
Data da última atualização: | 26/01/2017
Autoria: | FALEIRO, F. G.; KANNAN, B.; ALTPETER, F.
Afiliação: | FABIO GELAPE FALEIRO, CPAC; BASKARAN KANNAN; FREDY ALTPETER.
Título: | In vitro chromossome doubling of superior interspecific hybrids between elephantgrass and pearl millet using different antimitotic chemicals.
Ano de publicação: | 2011
Fonte/Imprenta: | In: ANNUAL FESC SUMMIT, 3., 2011, Florida. Oral and poster session: abstracts. [Gainesville: FESC, 2011].
Páginas: | p. 26.
Idioma: | Inglês
Notas: | Florida Energy Systems Consortium.
Conteúdo: | Elephantgrass (Pennisetum purpureum 2n = 4x = 28) produces large amounts of biomass in tropical and subtropical regions and is considered a prime candidate for production of lignocellulosic biofuel. Interspecific hybridization between elephantgrass and pearl millet (Pennisetum glaucum 2n = 2x = 14) may allow to improve important traits (e.g. drought tolerance, biomass quality and production). In contrast to elephantgrass and pearl millet, which produce large amounts of seeds, interspecific hybrids are male and female sterile due to triploidy (2n = 3x = 21) and do not produce seeds. Chromosome doubling of the triploid hybrids may restore seed production and fertility, allowing backcrosses with elephantgrass to further enhance biomass production and persistence. In this study, chromosome doubling was performed in vitro from the most persistent and productive interspecific hybrids. Callus was induced in vitro from cross-sections of the immature leaf whorl of five superior interspecific hybrids (MN 12, MN 18, MN 51, MN 54 and MN 55). Embryogenic calli were incubated for 48h with two alternative concentrations of the antimitotic agents Oryzalin or Trifluralin. Stomata size measurements and flow cytometry were performed following plant regeneration from embryogenic callus and transfer to soil. A total of 328 regenerated plants were obtained and 74 presented altered ploidy according to flow cytometer analysis. Stomata longitudinal diameter was a good indicator of altered ploidy. The treatment with 5 µM Oryzalin produced the highest number (55) of plants with altered ploidy. Chromosome doubling was obtained for all five superior interspecific hybrids. The highest numbers of plants with altered ploidy was obtained from MN 18 and MN 51 genotypes with of 29 and 27 plants, respectively. The plants with altered ploidy have been transferred to the field to evaluate agronomic performance and fertility. The best performing plants with recovered fertility will be used in further elephant grass breeding cycles.
Thesagro: | Pennisetum Purpureum.
Categoria do assunto: | --
Registro original: | Embrapa Cerrados (CPAC)
Exemplares: | CPAC - (33127-1) - UPC - PL - PP - S1513 -  - S1513 - DIS