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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
03/11/2010 |
Data da última atualização: |
22/08/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GOMES, K. A.; ALMEIDA, T. C.; GESTEIRA, A. da S.; LÔBO, I. P.; GUIMARÃES, A. C. R.; MIRANDA, A. B. de; VAN SLUYS, M. A.; CRUZ, R. S. da; CASCARDO, J. C. M.; CARELS, N. |
Afiliação: |
Kleber A. Gomes, UESC; Tiago C. Almeida, UESC; ABELMON DA SILVA GESTEIRA, CNPMF; Ivon Pinheiro Lobo, UESC; Ana Carolina R. Guimarães, FIOCRUZ; Antonio B. de Miranda, FIOCRUZ; Marie-Anne Van Sluys, USP; Rosenira Serpa da Cruz, UESC; Júlio Cézar Mattos Cascardo, UESC; Nicolas Carels, UESC. |
Título: |
ESTs from seeds to assist the selective breeding of Jatropha curcas L. for oil and active compounds. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Genomics Insights, v. 3, p. 29?56, 2010. |
ISSN: |
1178-6310 |
DOI: |
10.4137/GEI.S4340 |
Idioma: |
Inglês |
Notas: |
Diponível em:.Acesso em: 03 nov. 2010. |
Conteúdo: |
We report here on the characterization of a cDNA library from seeds of Jatropha curcas L. at three stages of fruit maturation before yellowing. We sequenced a total of 2200 clones and obtained a set of 931 non-redundant sequences (unigenes) after trimming and quality control, ie, 140 contigs and 791 singlets with PHRED quality $10. We found low levels of sequence redundancy and extensive metabolic coverage by homology comparison to GO. After comparison of 5841 non-redundant ESTs from a total of 13193 reads from GenBank with KEGG, we identified tags with nucleotide variations among J. curcas accessions for genes of fatty acid, terpene, alkaloid, quinone and hormone pathways of biosynthesis. More specifically, the expression level of four genes (palmitoyl-acyl carrier protein thioesterase, 3-ketoacyl-CoA thiolase B, lysophosphatidic acid acyltransferase and geranyl pyrophosphate synthase) measured by real- time PCR proved to be significantly different between leaves and fruits. Since the nucleotide polymorphism of these tags is associated to higher level of gene expression in fruits compared to leaves, we propose this approach to speed up the search for quantitative traits in selective breeding of J. curcas. We also discuss its potential utility for the selective breeding of economically important traits in J. curcas. |
Palavras-Chave: |
Biofuel; Melhoramento genetico vegatal; Pinhão manso; Terpenes. |
Thesagro: |
Biocombustível; Jatropha Curcas. |
Thesaurus Nal: |
alkaloids; fatty acids; genomics. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/23536/1/f-2328-GEI-ESTs-from-Seeds-to-Assist-the-Selective-Breeding-of-Jatropha-curcas-L.pdf-31711.pdf
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Marc: |
LEADER 02518naa a2200373 a 4500 001 1865928 005 2022-08-22 008 2010 bl uuuu u00u1 u #d 022 $a1178-6310 024 7 $a10.4137/GEI.S4340$2DOI 100 1 $aGOMES, K. A. 245 $aESTs from seeds to assist the selective breeding of Jatropha curcas L. for oil and active compounds.$h[electronic resource] 260 $c2010 500 $aDiponível em:<http://www.la-press.com/ests-from-seeds-to-assist-the-selective-breeding-of-jatropha-curcas-l--article-a2328>.Acesso em: 03 nov. 2010. 520 $aWe report here on the characterization of a cDNA library from seeds of Jatropha curcas L. at three stages of fruit maturation before yellowing. We sequenced a total of 2200 clones and obtained a set of 931 non-redundant sequences (unigenes) after trimming and quality control, ie, 140 contigs and 791 singlets with PHRED quality $10. We found low levels of sequence redundancy and extensive metabolic coverage by homology comparison to GO. After comparison of 5841 non-redundant ESTs from a total of 13193 reads from GenBank with KEGG, we identified tags with nucleotide variations among J. curcas accessions for genes of fatty acid, terpene, alkaloid, quinone and hormone pathways of biosynthesis. More specifically, the expression level of four genes (palmitoyl-acyl carrier protein thioesterase, 3-ketoacyl-CoA thiolase B, lysophosphatidic acid acyltransferase and geranyl pyrophosphate synthase) measured by real- time PCR proved to be significantly different between leaves and fruits. Since the nucleotide polymorphism of these tags is associated to higher level of gene expression in fruits compared to leaves, we propose this approach to speed up the search for quantitative traits in selective breeding of J. curcas. We also discuss its potential utility for the selective breeding of economically important traits in J. curcas. 650 $aalkaloids 650 $afatty acids 650 $agenomics 650 $aBiocombustível 650 $aJatropha Curcas 653 $aBiofuel 653 $aMelhoramento genetico vegatal 653 $aPinhão manso 653 $aTerpenes 700 1 $aALMEIDA, T. C. 700 1 $aGESTEIRA, A. da S. 700 1 $aLÔBO, I. P. 700 1 $aGUIMARÃES, A. C. R. 700 1 $aMIRANDA, A. B. de 700 1 $aVAN SLUYS, M. A. 700 1 $aCRUZ, R. S. da 700 1 $aCASCARDO, J. C. M. 700 1 $aCARELS, N. 773 $tGenomics Insights$gv. 3, p. 29?56, 2010.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registros recuperados : 4 | |
3. | | ALMEIDA, T. C. A.; FOLEGATTI, M. I. S.; FREIRE, M. T. A.; MADEIRA, M. S.; SILVA, F.; SILVA, M. A. A. P. Determinacao do perfil sensorial e parametros de qualidade de figos em calda produzidos pela industria brasileira. Ciencia e Tecnologia de Alimentos, Campinas, v.19, n.2, p.234-240, 1999.Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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4. | | GOMES, K. A.; ALMEIDA, T. C.; GESTEIRA, A. da S.; LÔBO, I. P.; GUIMARÃES, A. C. R.; MIRANDA, A. B. de; VAN SLUYS, M. A.; CRUZ, R. S. da; CASCARDO, J. C. M.; CARELS, N. ESTs from seeds to assist the selective breeding of Jatropha curcas L. for oil and active compounds. Genomics Insights, v. 3, p. 29?56, 2010. Diponível em:.Acesso em: 03 nov. 2010.Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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Registros recuperados : 4 | |
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