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Registro Completo |
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Biblioteca(s): |
Embrapa Agropecuária Oeste. |
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Data corrente: |
25/08/2004 |
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Data da última atualização: |
25/08/2004 |
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Autoria: |
ALMEIDA, R. P. de. |
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Título: |
Trichogramma and its relationship with Wolbachia: identification of Trichogramma species, phylogeny, transfer and costs of Wolbachia symbionts. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
2004. |
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Páginas: |
142 p. |
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Idioma: |
Inglês |
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Notas: |
Thesis (Doctor) - Wageningen Universiteit, Wageningen, 2004. |
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Conteúdo: |
Identification of Trichogramma individuals has long been very difficult. No reliable character was known for the identification of species in this genus, until the utility of male genitalia was recognized in 1968, some 135 years after the first Trichogramma was described (T. evanescens Westwood, 1833). However, the presence of completely parthenogenetic lines in this species remained an identification problem because the no reliable female characters exist that allowed identification. The discovery of Wolbachia as a cause for the complete parthenogenesis in this genus has allowed the identification of such thelytokous lines. Antibiotic treatment could revertWolbachia infected thelytokous species to sexual ones, thus producing males and allowing their identification in the morphological system. However, the lack of morphological identification of Trichogramma females was only solved with the use of molecular techniques based on rDNA sequencing of the internal transcribed spacer 2 (ITS2) region. Here, this technique was proved to be a reliable tool for the identification of T. cacoeciae, a thelytokous species where the parthenogenetic reproduction is not due to Wolbachia infection. In this thesis we report the first record of this species in Peru (Chapter 2). This species is the only elytokousTrichogramma known in which Wolbachia is not present. T. cacoeciaepresence in South America is discussed. Identification of seventeen native/introduced Trichogramma species using rDNA sequences was done. A molecular key based on restriction analysis allowed identification of the species through the size of the PCR product and the generation of the restriction patterns. Thelytoky in Trichogramma species caused by Wolbachia collected in Peru, Colombia and USA were reported (Chapter 3).Phylogenetic analysis and comparison of DNA sequences of the Wolbachiawsp gene allowed for the recognition of new Wolbachia group ?Ato? in the clade of Wolbachia that infect Trichogramma species. The construction of Wolbachiaphylogenetic tree showed four distinct groups. The similarities in the Wolbachiasequences for the studied groups suggested the possibility of horizontal transmission between Trichogramma species (Chapter 4). Natural inter- and intraspecific horizontal transfer of PI Wolbachiabetween wasps of the genus Trichogramma was shown. Wolbachiainfection in uninfected Trichogramma females was possible when infected and uninfected immature wasps shared the same host egg. On the whole, intraspecificinterspecific transfer (Chapter 5). In T. atopovirilia, Wolbachia infection did not lead to any negative effect on the walking activity, walking speed or other behaviour components studied (Chapter 6).Finally, an overview of the most important outcomes and conclusions of this thesis is presented (Chapter 7). MenosIdentification of Trichogramma individuals has long been very difficult. No reliable character was known for the identification of species in this genus, until the utility of male genitalia was recognized in 1968, some 135 years after the first Trichogramma was described (T. evanescens Westwood, 1833). However, the presence of completely parthenogenetic lines in this species remained an identification problem because the no reliable female characters exist that allowed identification. The discovery of Wolbachia as a cause for the complete parthenogenesis in this genus has allowed the identification of such thelytokous lines. Antibiotic treatment could revertWolbachia infected thelytokous species to sexual ones, thus producing males and allowing their identification in the morphological system. However, the lack of morphological identification of Trichogramma females was only solved with the use of molecular techniques based on rDNA sequencing of the internal transcribed spacer 2 (ITS2) region. Here, this technique was proved to be a reliable tool for the identification of T. cacoeciae, a thelytokous species where the parthenogenetic reproduction is not due to Wolbachia infection. In this thesis we report the first record of this species in Peru (Chapter 2). This species is the only elytokousTrichogramma known in which Wolbachia is not present. T. cacoeciaepresence in South America is discussed. Identification of seventeen native/introduced Trichogramma species using rDNA se... Mostrar Tudo |
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Palavras-Chave: |
Análise filogenética; Morfologia; Polimerase. |
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Thesagro: |
Controle Biológico; DNA; Espécie; Inseto; Reprodução. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03515nam a2200229 a 4500
001 1248549
005 2004-08-25
008 2004 bl uuuu m 00u1 u #d
100 1 $aALMEIDA, R. P. de
245 $aTrichogramma and its relationship with Wolbachia$bidentification of Trichogramma species, phylogeny, transfer and costs of Wolbachia symbionts.
260 $a2004.$c2004
300 $a142 p.
500 $aThesis (Doctor) - Wageningen Universiteit, Wageningen, 2004.
520 $aIdentification of Trichogramma individuals has long been very difficult. No reliable character was known for the identification of species in this genus, until the utility of male genitalia was recognized in 1968, some 135 years after the first Trichogramma was described (T. evanescens Westwood, 1833). However, the presence of completely parthenogenetic lines in this species remained an identification problem because the no reliable female characters exist that allowed identification. The discovery of Wolbachia as a cause for the complete parthenogenesis in this genus has allowed the identification of such thelytokous lines. Antibiotic treatment could revertWolbachia infected thelytokous species to sexual ones, thus producing males and allowing their identification in the morphological system. However, the lack of morphological identification of Trichogramma females was only solved with the use of molecular techniques based on rDNA sequencing of the internal transcribed spacer 2 (ITS2) region. Here, this technique was proved to be a reliable tool for the identification of T. cacoeciae, a thelytokous species where the parthenogenetic reproduction is not due to Wolbachia infection. In this thesis we report the first record of this species in Peru (Chapter 2). This species is the only elytokousTrichogramma known in which Wolbachia is not present. T. cacoeciaepresence in South America is discussed. Identification of seventeen native/introduced Trichogramma species using rDNA sequences was done. A molecular key based on restriction analysis allowed identification of the species through the size of the PCR product and the generation of the restriction patterns. Thelytoky in Trichogramma species caused by Wolbachia collected in Peru, Colombia and USA were reported (Chapter 3).Phylogenetic analysis and comparison of DNA sequences of the Wolbachiawsp gene allowed for the recognition of new Wolbachia group ?Ato? in the clade of Wolbachia that infect Trichogramma species. The construction of Wolbachiaphylogenetic tree showed four distinct groups. The similarities in the Wolbachiasequences for the studied groups suggested the possibility of horizontal transmission between Trichogramma species (Chapter 4). Natural inter- and intraspecific horizontal transfer of PI Wolbachiabetween wasps of the genus Trichogramma was shown. Wolbachiainfection in uninfected Trichogramma females was possible when infected and uninfected immature wasps shared the same host egg. On the whole, intraspecificinterspecific transfer (Chapter 5). In T. atopovirilia, Wolbachia infection did not lead to any negative effect on the walking activity, walking speed or other behaviour components studied (Chapter 6).Finally, an overview of the most important outcomes and conclusions of this thesis is presented (Chapter 7).
650 $aControle Biológico
650 $aDNA
650 $aEspécie
650 $aInseto
650 $aReprodução
653 $aAnálise filogenética
653 $aMorfologia
653 $aPolimerase
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Registro original: |
Embrapa Agropecuária Oeste (CPAO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
30/03/2012 |
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Data da última atualização: |
08/06/2017 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
EMBRAPA ALGODÃO. |
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Afiliação: |
Embrapa Algodão. |
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Título: |
Relatório de Atividades Embrapa Algodão 2010. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Campina Grande: Embrapa Algodão, 2011. |
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Páginas: |
42 p. |
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Série: |
(Embrapa Algodão. Documentos, 239). |
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ISSN: |
0103-0205 |
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Idioma: |
Português |
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Palavras-Chave: |
Gestão organizacional; Pesquisa Desenvolvimento e Inovação; Pesquisa, Desenvolvimento e Inovação; Relatório de atividades; Transferência de Tecnologia e Conhecimento. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/57548/1/DOC239.pdf
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Marc: |
LEADER 00625nam a2200193 a 4500
001 1921035
005 2017-06-08
008 2011 bl uuuu u0uu1 u #d
022 $a0103-0205
100 1 $aEMBRAPA ALGODÃO.
245 $aRelatório de Atividades Embrapa Algodão 2010.
260 $aCampina Grande: Embrapa Algodão$c2011
300 $a42 p.
490 $a(Embrapa Algodão. Documentos, 239).
653 $aGestão organizacional
653 $aPesquisa Desenvolvimento e Inovação
653 $aPesquisa, Desenvolvimento e Inovação
653 $aRelatório de atividades
653 $aTransferência de Tecnologia e Conhecimento
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