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Registro Completo |
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
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Data corrente: |
29/11/2018 |
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Data da última atualização: |
04/12/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALMEIDA, R. E. M. de; FAVARIN, J. L.; OTTO, R.; FRANCO, H.; REIS, A. F. B.; MOREIRA, L. A.; TRIVELIN, P. |
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Afiliação: |
RODRIGO ESTEVAM MUNHOZ DE ALMEIDA, CNPASA; JOSE LAERCIO FAVARIN, ESALQ; RAFEL OTTO, ESALQ; HENRIQUE FRANCO; ANDRÉ FROES BORJA REIS, ESALQ; LILIAN ANGELICA MOREIRA, ESALQ; PAULO TRIVELIN, CENA. |
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Título: |
Nitrogen recovery efficiency for corn intercropped with palisade grass. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Bragantia, v. 77, n. 4, p. 557-566, 2018. |
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DOI: |
10.1590/1678-4499.2017242 |
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Idioma: |
Inglês |
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Conteúdo: |
Intercropping corn and palisade grass is a technique to increase straw production, soil C contents, nutrient cycling and crop yield. However, concerns arise from nitrogen (N) uptake by the intercropping crop causing reduction in the yield of the corn. Our objective was to evaluate N recovery efficiency (NRE), and the N dynamics in the soil-plant system in corn intercropped with palisade grass. A field trial was carried out in Bahia, Brazil, evaluating two cropping systems: corn (monoculture) and corn intercropped with palisade grass sowed between rows on the same day as the corn crop, with four replicates in a completely randomized block design. Nitrogen (150 kg∙ha?1of 15N-urea) was applied at sowing to determine NRE, which means the amounts of N-fertilizer uptake in corn and palisade grass, the amounts of N-fertilizer in soil and the 15N-fertilizer balance. Neither the NRE (63.3% in monoculture and 57.2% in intercropping) nor corn grain yield (9,800 kg∙ha?1 in monoculture and 9,671 kg∙ha?1 in intercropping) was affected by intercropping, which accumulated only 2.1 kg∙ha?1 of N-fertilizer or 1.4% N rate. In addition, palisade grass yielded 2,265 kg∙ha?1 of dry matter. The balance indicated that 82.4% of N-fertilizer was recovered in the monoculture and 86.9% in the intercropping. Intercropping palisade grass does not affect grain yield or N corn nutrition and has the potential to increase straw production contributing to maintenance of no-till. |
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Thesagro: |
Cerrado; Milho; Nitrogênio; Plantio Direto; Zea Mays. |
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Thesaurus Nal: |
Corn; Grasses; Nitrogen; No-tillage; Urochloa ruziziensis. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/187284/1/CNPASA-2018-bragantia.pdf
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Marc: |
LEADER 02349naa a2200325 a 4500
001 2100320
005 2018-12-04
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1590/1678-4499.2017242$2DOI
100 1 $aALMEIDA, R. E. M. de
245 $aNitrogen recovery efficiency for corn intercropped with palisade grass.$h[electronic resource]
260 $c2018
520 $aIntercropping corn and palisade grass is a technique to increase straw production, soil C contents, nutrient cycling and crop yield. However, concerns arise from nitrogen (N) uptake by the intercropping crop causing reduction in the yield of the corn. Our objective was to evaluate N recovery efficiency (NRE), and the N dynamics in the soil-plant system in corn intercropped with palisade grass. A field trial was carried out in Bahia, Brazil, evaluating two cropping systems: corn (monoculture) and corn intercropped with palisade grass sowed between rows on the same day as the corn crop, with four replicates in a completely randomized block design. Nitrogen (150 kg∙ha?1of 15N-urea) was applied at sowing to determine NRE, which means the amounts of N-fertilizer uptake in corn and palisade grass, the amounts of N-fertilizer in soil and the 15N-fertilizer balance. Neither the NRE (63.3% in monoculture and 57.2% in intercropping) nor corn grain yield (9,800 kg∙ha?1 in monoculture and 9,671 kg∙ha?1 in intercropping) was affected by intercropping, which accumulated only 2.1 kg∙ha?1 of N-fertilizer or 1.4% N rate. In addition, palisade grass yielded 2,265 kg∙ha?1 of dry matter. The balance indicated that 82.4% of N-fertilizer was recovered in the monoculture and 86.9% in the intercropping. Intercropping palisade grass does not affect grain yield or N corn nutrition and has the potential to increase straw production contributing to maintenance of no-till.
650 $aCorn
650 $aGrasses
650 $aNitrogen
650 $aNo-tillage
650 $aUrochloa ruziziensis
650 $aCerrado
650 $aMilho
650 $aNitrogênio
650 $aPlantio Direto
650 $aZea Mays
700 1 $aFAVARIN, J. L.
700 1 $aOTTO, R.
700 1 $aFRANCO, H.
700 1 $aREIS, A. F. B.
700 1 $aMOREIRA, L. A.
700 1 $aTRIVELIN, P.
773 $tBragantia$gv. 77, n. 4, p. 557-566, 2018.
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Registro original: |
Embrapa Pesca e Aquicultura (CNPASA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
19/04/2023 |
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Data da última atualização: |
19/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
RIEGER, J. S. G.; MANTOVANI, C.; SUNIGA, P. A. P.; PINTO, I. B.; SOUSA, G. A. A.; GAVA, D.; CANTAO, M. E.; SANTOS, L. R.; ARAÚJO, F. R.; ZANELLA, J. R. C. |
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Afiliação: |
DANIELLE GAVA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; JANICE REIS CIACCI ZANELLA, CNPSA. |
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Título: |
Standardization of ELISA with senecavirus A recombinant VP2 protein and its use in swine herds in Brazil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 22, n. 1, ed. gmr19118, 2023. |
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DOI: |
http://dx.doi.org/10.4238/gmr19118 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here was able to detect specific SVA antibodies in acute disease and recovered pigs, and no cross-reactivity with VSV was observed. This assay has potential as a useful tool for monitoring SVA infection and could help to improve disease diagnosis. MenosAbstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here wa... Mostrar Tudo |
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Thesagro: |
Diagnostico. |
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Thesaurus NAL: |
Senecavirus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1153239/1/final10100.pdf
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Marc: |
LEADER 02545naa a2200265 a 4500
001 2153239
005 2023-04-19
008 2023 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/gmr19118$2DOI
100 1 $aRIEGER, J. S. G.
245 $aStandardization of ELISA with senecavirus A recombinant VP2 protein and its use in swine herds in Brazil.$h[electronic resource]
260 $c2023
520 $aAbstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here was able to detect specific SVA antibodies in acute disease and recovered pigs, and no cross-reactivity with VSV was observed. This assay has potential as a useful tool for monitoring SVA infection and could help to improve disease diagnosis.
650 $aSenecavirus
650 $aDiagnostico
700 1 $aMANTOVANI, C.
700 1 $aSUNIGA, P. A. P.
700 1 $aPINTO, I. B.
700 1 $aSOUSA, G. A. A.
700 1 $aGAVA, D.
700 1 $aCANTAO, M. E.
700 1 $aSANTOS, L. R.
700 1 $aARAÚJO, F. R.
700 1 $aZANELLA, J. R. C.
773 $tGenetics and Molecular Research$gv. 22, n. 1, ed. gmr19118, 2023.
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Embrapa Suínos e Aves (CNPSA) |
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