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Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Ocidental. |
Data corrente: |
30/03/2011 |
Data da última atualização: |
02/02/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CHEN, S. X.; BOGERD, J.; ANDERSSON, E.; ALMEIDA, F. F. L. de; TARANGER, G. L.; SCHULZ, R. W. |
Afiliação: |
SHI X CHEN, UTRECHT UNIVERSITY; JAN BOGERD, UTRECHT UNIVERSITY; EVA ANDERSSON, INSTITUTE OF MARINE RESEARCH; FERNANDA FERREIRA LOUREIRO DE ALMEIDA, CPAA; GEIR LASSE TARANGER, INSTITUTE OF MARINE RESEARCH; RUDIGER W. SCHULZ, UTRECHT UNIVERSITY. |
Título: |
Cloning, pharmacological characterization, and expression analysis of Atlantic salmon (Salmo salar L.) nuclear progesterone receptor. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Reproduction, v. 141, p. 491-500, Apr. 2011. |
DOI: |
10.1530/REP-10-0224 |
Idioma: |
Inglês |
Conteúdo: |
To better understand the role(s) of progestogens during early stages of spermatogenesis, we carried out studies on the nuclear progesterone receptor (Pgr) of the Atlantic salmon. Its open-reading frame shows the highest similarity with other piscine Pgr proteins. When expressed in mammalian cells, salmon Pgr exhibited progestogen-specific, dose-dependent induction of reporter gene expression, with 17?,20?-dihydroxy-4-pregnen-3-one (DHP) showing the highest potency. We then analyzed testicular pgr mRNA and DHP plasma levels in animals during the onset of spermatogenesis, which were exposed to natural light or to constant light, to induce significant differences in testis growth. Grouping of the animals according to their progress through spermatogenesis showed that testicular pgr mRNA levels as well as DHP plasma levels first increased when germ cells had reached the stage of late type B spermatogonia and further increased when entered meiosis, i.e. when spermatocytes were present. However, in situ hybridization studies revealed that pgr mRNA expression was restricted to Sertoli cells, with a strong signal in Sertoli cells contacting type A/early type B spermatogonia, while Sertoli cells contacting larger germ cell clones with further differentiated stages (e.g. late type B spermatogonia) were less intensely/not stained. We conclude that the increase in pgr mRNA levels per pair of testis reflects, at least in part, the increased number of Sertoli cells enveloping type A and early type B spermatogonia. We propose that Sertoli cell-expressed Pgr may mediate DHP-stimulated early steps in spermatogenesis in Atlantic salmon, such as an increase in the number of new spermatogonial cysts. MenosTo better understand the role(s) of progestogens during early stages of spermatogenesis, we carried out studies on the nuclear progesterone receptor (Pgr) of the Atlantic salmon. Its open-reading frame shows the highest similarity with other piscine Pgr proteins. When expressed in mammalian cells, salmon Pgr exhibited progestogen-specific, dose-dependent induction of reporter gene expression, with 17?,20?-dihydroxy-4-pregnen-3-one (DHP) showing the highest potency. We then analyzed testicular pgr mRNA and DHP plasma levels in animals during the onset of spermatogenesis, which were exposed to natural light or to constant light, to induce significant differences in testis growth. Grouping of the animals according to their progress through spermatogenesis showed that testicular pgr mRNA levels as well as DHP plasma levels first increased when germ cells had reached the stage of late type B spermatogonia and further increased when entered meiosis, i.e. when spermatocytes were present. However, in situ hybridization studies revealed that pgr mRNA expression was restricted to Sertoli cells, with a strong signal in Sertoli cells contacting type A/early type B spermatogonia, while Sertoli cells contacting larger germ cell clones with further differentiated stages (e.g. late type B spermatogonia) were less intensely/not stained. We conclude that the increase in pgr mRNA levels per pair of testis reflects, at least in part, the increased number of Sertoli cells enveloping type A and e... Mostrar Tudo |
Thesagro: |
Reprodução. |
Categoria do assunto: |
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Marc: |
LEADER 02334naa a2200205 a 4500 001 1884099 005 2018-02-02 008 2011 bl uuuu u00u1 u #d 024 7 $a10.1530/REP-10-0224$2DOI 100 1 $aCHEN, S. X. 245 $aCloning, pharmacological characterization, and expression analysis of Atlantic salmon (Salmo salar L.) nuclear progesterone receptor. 260 $c2011 520 $aTo better understand the role(s) of progestogens during early stages of spermatogenesis, we carried out studies on the nuclear progesterone receptor (Pgr) of the Atlantic salmon. Its open-reading frame shows the highest similarity with other piscine Pgr proteins. When expressed in mammalian cells, salmon Pgr exhibited progestogen-specific, dose-dependent induction of reporter gene expression, with 17?,20?-dihydroxy-4-pregnen-3-one (DHP) showing the highest potency. We then analyzed testicular pgr mRNA and DHP plasma levels in animals during the onset of spermatogenesis, which were exposed to natural light or to constant light, to induce significant differences in testis growth. Grouping of the animals according to their progress through spermatogenesis showed that testicular pgr mRNA levels as well as DHP plasma levels first increased when germ cells had reached the stage of late type B spermatogonia and further increased when entered meiosis, i.e. when spermatocytes were present. However, in situ hybridization studies revealed that pgr mRNA expression was restricted to Sertoli cells, with a strong signal in Sertoli cells contacting type A/early type B spermatogonia, while Sertoli cells contacting larger germ cell clones with further differentiated stages (e.g. late type B spermatogonia) were less intensely/not stained. We conclude that the increase in pgr mRNA levels per pair of testis reflects, at least in part, the increased number of Sertoli cells enveloping type A and early type B spermatogonia. We propose that Sertoli cell-expressed Pgr may mediate DHP-stimulated early steps in spermatogenesis in Atlantic salmon, such as an increase in the number of new spermatogonial cysts. 650 $aReprodução 700 1 $aBOGERD, J. 700 1 $aANDERSSON, E. 700 1 $aALMEIDA, F. F. L. de 700 1 $aTARANGER, G. L. 700 1 $aSCHULZ, R. W. 773 $tReproduction$gv. 141, p. 491-500, Apr. 2011.
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1. | | HOFER, H. Carbon and nitrogen analyses. In: SHIFT PROJECT ENV 52 (Manaus-AB). Soil fauna and litter decomposition in primary and secondary forests and a mixed culture system in Amazonia. Manaus: SMNK/Embrapa Amazonia Ocidental, 1999. p. 264-279. Final Report 1996-1999.Biblioteca(s): Embrapa Amazônia Ocidental. |
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2. | | HOFER, H.; LUIZAO, F. Decompositon in letterbagas and mini-containers. In: SHIFT PROJECT ENV 52 (Manaus-AB). Soil fauna and litter decomposition in primary and secondary forests and a mixed culture system in Amazonia. Manaus: SMNK/Embrapa Amazonia Ocidental, 1999. p. 217-229. Final Report 1996-1999.Biblioteca(s): Embrapa Amazônia Ocidental. |
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8. | | MARTIUS, C.; HOFER, H.; AMELUNG, W. Phenole in Streubeutein. In: SHIFT PROJECT ENV 52 (Manaus-AB). Soil fauna and litter decomposition in primary and secondary forests and a mixed culture system in Amazonia. Manaus: SMNK/Embrapa Amazonia Ocidental, 1999. p. 280-282. Final Report 1996-1999.Biblioteca(s): Embrapa Amazônia Ocidental. |
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12. | | FORSTER, B.; GARCIA, M.; HOFER, H.; MORGAN, E.; ROMBKE, J. Tropical terrestrial model ecosystems for evaluation of soil fauna and leaf litter quality effects on litter consumption, soil microbial biomass and plant growth. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 8, p. 1063-1071, ago. 2009 Título em português: Efeitos de fauna de solo e qualidade de liteira sobre o consumo, biomassa microbiana e crescimento de plantas em modelo de ecossistemas terrestres tropicais.Biblioteca(s): Embrapa Unidades Centrais. |
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17. | | BECK, L.; HOFER, H.; MARTIUS, C.; GARCIA, M. B.; FRANKLIN, E.; ROMBKE, J. Soil fauna and litter decomposition in primary and secondary forests and a polyculture system in Amazonia - study design and methodology. In: SHIFT - WORKSHOP, 3., 1998, Manaus. Proceedings... Bonn: BMBF, 1998. p. 463-469.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Ocidental. |
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18. | | GARCIA, M.; HOFER, H.; MARTIUS, C.; ROMBKE, J.; OTT, R.; BECK, L. SHIFT project ENV 52: soil fauna and litter decomposition. The use of adapted soil biological methods to study macrofauna in Amazonian rain forests. In: SHIFT WORKSHOP, 3., 1998, Manaus. Program, abstracts of presentations and posters... Hamburg: GKSS, 1998. p. C44.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Ocidental. |
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19. | | FÖRSTER, B.; GARCIA, M. V. B.; HÖFER, H.; MORGAN, E.; RÖMBKE, J. Tropical terrestrial model ecosystems for evaluation of soil fauna and leaf litter quality effects on litter consumption, soil microbial biomass and plant growth. Pesquisa Agropecuária Brasileira, v. 44, n. 8, p. 1063-1071, ago. 2009. XV International Colloquium on Soil Zoology and XII International Colloquium on Apterygota.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Amazônia Ocidental. |
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20. | | HOPP, P. W.; OTTERMANNS, R.; HÖFER, H.; MARQUES, R.; ROB-NICKOLL, M. Recovery of leaf-litter Beetles during Forest Regeneration in the Atlantic Forest of Southern Brazil. In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.Biblioteca(s): Embrapa Florestas. |
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Registros recuperados : 35 | |
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