Data/hora: 18/06/2024 00:06:15

Biblioteca(s): 	Embrapa Amazônia Ocidental.	
Data corrente: 	30/03/2011	
Data da última atualização: 	02/02/2018	
Autoria: 	CHEN, S. X.; BOGERD, J.; ANDERSSON, E.; ALMEIDA, F. F. L. de; TARANGER, G. L.; SCHULZ, R. W.	
Afiliação: 	SHI X CHEN, UTRECHT UNIVERSITY; JAN BOGERD, UTRECHT UNIVERSITY; EVA ANDERSSON, INSTITUTE OF MARINE RESEARCH; FERNANDA FERREIRA LOUREIRO DE ALMEIDA, CPAA; GEIR LASSE TARANGER, INSTITUTE OF MARINE RESEARCH; RUDIGER W. SCHULZ, UTRECHT UNIVERSITY.	
Título: 	Cloning, pharmacological characterization, and expression analysis of Atlantic salmon (Salmo salar L.) nuclear progesterone receptor.	
Ano de publicação: 	2011	
Fonte/Imprenta: 	Reproduction, v. 141, p. 491-500, Apr. 2011.	
DOI: 	10.1530/REP-10-0224	
Idioma: 	Inglês	
Conteúdo: 	To better understand the role(s) of progestogens during early stages of spermatogenesis, we carried out studies on the nuclear progesterone receptor (Pgr) of the Atlantic salmon. Its open-reading frame shows the highest similarity with other piscine Pgr proteins. When expressed in mammalian cells, salmon Pgr exhibited progestogen-specific, dose-dependent induction of reporter gene expression, with 17?,20?-dihydroxy-4-pregnen-3-one (DHP) showing the highest potency. We then analyzed testicular pgr mRNA and DHP plasma levels in animals during the onset of spermatogenesis, which were exposed to natural light or to constant light, to induce significant differences in testis growth. Grouping of the animals according to their progress through spermatogenesis showed that testicular pgr mRNA levels as well as DHP plasma levels first increased when germ cells had reached the stage of late type B spermatogonia and further increased when entered meiosis, i.e. when spermatocytes were present. However, in situ hybridization studies revealed that pgr mRNA expression was restricted to Sertoli cells, with a strong signal in Sertoli cells contacting type A/early type B spermatogonia, while Sertoli cells contacting larger germ cell clones with further differentiated stages (e.g. late type B spermatogonia) were less intensely/not stained. We conclude that the increase in pgr mRNA levels per pair of testis reflects, at least in part, the increased number of Sertoli cells enveloping type A and early type B spermatogonia. We propose that Sertoli cell-expressed Pgr may mediate DHP-stimulated early steps in spermatogenesis in Atlantic salmon, such as an increase in the number of new spermatogonial cysts.	
Thesagro: 	Reprodução.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Amazônia Ocidental (CPAA)	
Exemplares: 	CPAA - (23327-1) - UPC - AP - PP - S8813 -  - S8813 - DIS