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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
24/08/2017 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. |
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Afiliação: |
Helena Fabiana Reis de Almeida Saraiva, Universidade de São Paulo (USA) - Pirassununga, SP, Brasil; Ribrio Ivan Tavares Pereira Batista, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brasil; Vivian Angélico Pereira Alfradique, UFF - Niterói, RJ, Brasil; Pedro Henrique Nicolau Pinto, UFF - Niterói, RJ, Brasil; Lilian Santos Ribeiro, UFF - Niterói, RJ, Brasil; CLARA SLADE OLIVEIRA, CNPGL; Joanna Maria Gonçalves de Souza Fabjan, UFF - Niterói, RJ, Brasil; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Zandonadi Brandão, UFF - Niterói, RJ, Brasil. |
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Título: |
L-carnitine supplementation during vitrification did not improve survival and quality rates, but altered CrAT and PRDX1 expression in in vivo-produced ovine embryos. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Animal Reprodroduction, v. 14, n. 3, p. 898, Jul./Sept. 2017. |
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Idioma: |
Inglês |
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Notas: |
Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts. |
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Conteúdo: |
Embryo cryodamage is observed mainly at metabolic and molecular aspects and it impairs post warming quality and survival rates. This study aimed to evaluate the effect of L-carnitine (LC) supplementation during either vitrification or post warming solutions on the 6-7th day of in vivo-produced ovine embryos. LC (3.72 mM) was added to vitrification (Experiment 1; C1: control; LC1: supplemented embryos) or warming solutions (Experiment 2; C2; LC2). In vitro culture (IVC) of warmed embryos was performed for 72 h at 38,5 °C, 5% CO2 and 5% O2 to evaluate survival rates in both Experiments. In Experiment 1, reactive oxygen species (ROS) levels were measured by CellROX Green staining, total cell number (TCN) by Hoechst 33342, number of apoptotic cells by caspase-3 immunofluorescence staining protocol, apoptotic index evaluation in both groups. Gene expression analysis of carnitine palmitoyltransferase 1 and 2 (CPT1 and CPT2), carnitine O-acyltransferase (CrAT) and peroxiredoxin 1 (PRDX1), were performed by RT-qPCR (ACTB as endogenous control) in Experiments 1 and 2 and results were compared to fresh embryos (FE). Averages of survival rates were compared by the Chi-Square test. Means of TCN, apoptotic cells, apoptotic index and fluorescence intensity were compared by Student's t-test, at 5% significance level. Survival rates were similar between groups (p> 0.05) in Experiments 1 (68.7%, C1 vs 81.8%, LC1) and 2 (48.5%, C2 vs 64.7%, LC2). In Experiment 1, ROS levels at 24 h of IVC (85.83 ± 68.37 x 1010, C1 vs 89.04 ± 84.48 x 1010, LC1), total cell number at 24 h (89 ± 22, C1 vs 82.2 ± 28, LC1) and 72 h (86 ± 19.9, C1 vs 68.5 ± 25.26, LC1), apoptotic cells (3.75 ± 1.48, C1 vs 4.50 ± 4.72, LC1) and apoptotic index (4.37 ± 1.45, C1 vs 5.23 ± 4.72, LC1) at 72 h of IVC did not differ (p> 0.05) between C1 and LC1. Gene expression analysis showed no differences in CPT1 and CPT2 mRNA relative abundance in embryos of both experiments compared to FE, however, CrAT was downregulated (p< 0.05) in C1 and PRDX1 was downregulated (p< 0.05) in both C1 and LC1, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p< 0.05) in C2 and CrAT was downregulated (p< 0.05) in LC2, in relation to FE. In conclusion, although the short-term LC supplementation at 3.72 mM during cryopreservation did not improve post-warming survival and morphological parameters of the evaluated embryos, it was able to modulate expression of genes related to energy homeostasis (CrAT) and oxidative stress (PRDX1), proving to be beneficial, in both forms of supplementation, to in vivo-produced ovine embryos.". MenosEmbryo cryodamage is observed mainly at metabolic and molecular aspects and it impairs post warming quality and survival rates. This study aimed to evaluate the effect of L-carnitine (LC) supplementation during either vitrification or post warming solutions on the 6-7th day of in vivo-produced ovine embryos. LC (3.72 mM) was added to vitrification (Experiment 1; C1: control; LC1: supplemented embryos) or warming solutions (Experiment 2; C2; LC2). In vitro culture (IVC) of warmed embryos was performed for 72 h at 38,5 °C, 5% CO2 and 5% O2 to evaluate survival rates in both Experiments. In Experiment 1, reactive oxygen species (ROS) levels were measured by CellROX Green staining, total cell number (TCN) by Hoechst 33342, number of apoptotic cells by caspase-3 immunofluorescence staining protocol, apoptotic index evaluation in both groups. Gene expression analysis of carnitine palmitoyltransferase 1 and 2 (CPT1 and CPT2), carnitine O-acyltransferase (CrAT) and peroxiredoxin 1 (PRDX1), were performed by RT-qPCR (ACTB as endogenous control) in Experiments 1 and 2 and results were compared to fresh embryos (FE). Averages of survival rates were compared by the Chi-Square test. Means of TCN, apoptotic cells, apoptotic index and fluorescence intensity were compared by Student's t-test, at 5% significance level. Survival rates were similar between groups (p> 0.05) in Experiments 1 (68.7%, C1 vs 81.8%, LC1) and 2 (48.5%, C2 vs 64.7%, LC2). In Experiment 1, ROS levels at 24 h of IVC (85... Mostrar Tudo |
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Palavras-Chave: |
Animal embryos; Santa Inês; Survival. |
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Thesaurus Nal: |
Carnitine; Cell culture; Cryopreservation; Ewes; Gene expression; In vitro culture; Sheep; Vitrification. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163011/1/CNPC-2017-L-carnitine.pdf
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Marc: |
LEADER 03898nam a2200361 a 4500
001 2074433
005 2019-09-23
008 2017 bl uuuu u00u1 u #d
100 1 $aSARAIVA, H. F. R. de A.
245 $aL-carnitine supplementation during vitrification did not improve survival and quality rates, but altered CrAT and PRDX1 expression in in vivo-produced ovine embryos.$h[electronic resource]
260 $aAnimal Reprodroduction, v. 14, n. 3, p. 898, Jul./Sept. 2017.$c2017
500 $aProceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.
520 $aEmbryo cryodamage is observed mainly at metabolic and molecular aspects and it impairs post warming quality and survival rates. This study aimed to evaluate the effect of L-carnitine (LC) supplementation during either vitrification or post warming solutions on the 6-7th day of in vivo-produced ovine embryos. LC (3.72 mM) was added to vitrification (Experiment 1; C1: control; LC1: supplemented embryos) or warming solutions (Experiment 2; C2; LC2). In vitro culture (IVC) of warmed embryos was performed for 72 h at 38,5 °C, 5% CO2 and 5% O2 to evaluate survival rates in both Experiments. In Experiment 1, reactive oxygen species (ROS) levels were measured by CellROX Green staining, total cell number (TCN) by Hoechst 33342, number of apoptotic cells by caspase-3 immunofluorescence staining protocol, apoptotic index evaluation in both groups. Gene expression analysis of carnitine palmitoyltransferase 1 and 2 (CPT1 and CPT2), carnitine O-acyltransferase (CrAT) and peroxiredoxin 1 (PRDX1), were performed by RT-qPCR (ACTB as endogenous control) in Experiments 1 and 2 and results were compared to fresh embryos (FE). Averages of survival rates were compared by the Chi-Square test. Means of TCN, apoptotic cells, apoptotic index and fluorescence intensity were compared by Student's t-test, at 5% significance level. Survival rates were similar between groups (p> 0.05) in Experiments 1 (68.7%, C1 vs 81.8%, LC1) and 2 (48.5%, C2 vs 64.7%, LC2). In Experiment 1, ROS levels at 24 h of IVC (85.83 ± 68.37 x 1010, C1 vs 89.04 ± 84.48 x 1010, LC1), total cell number at 24 h (89 ± 22, C1 vs 82.2 ± 28, LC1) and 72 h (86 ± 19.9, C1 vs 68.5 ± 25.26, LC1), apoptotic cells (3.75 ± 1.48, C1 vs 4.50 ± 4.72, LC1) and apoptotic index (4.37 ± 1.45, C1 vs 5.23 ± 4.72, LC1) at 72 h of IVC did not differ (p> 0.05) between C1 and LC1. Gene expression analysis showed no differences in CPT1 and CPT2 mRNA relative abundance in embryos of both experiments compared to FE, however, CrAT was downregulated (p< 0.05) in C1 and PRDX1 was downregulated (p< 0.05) in both C1 and LC1, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p< 0.05) in C2 and CrAT was downregulated (p< 0.05) in LC2, in relation to FE. In conclusion, although the short-term LC supplementation at 3.72 mM during cryopreservation did not improve post-warming survival and morphological parameters of the evaluated embryos, it was able to modulate expression of genes related to energy homeostasis (CrAT) and oxidative stress (PRDX1), proving to be beneficial, in both forms of supplementation, to in vivo-produced ovine embryos.".
650 $aCarnitine
650 $aCell culture
650 $aCryopreservation
650 $aEwes
650 $aGene expression
650 $aIn vitro culture
650 $aSheep
650 $aVitrification
653 $aAnimal embryos
653 $aSanta Inês
653 $aSurvival
700 1 $aBATISTA, R. I. T. P.
700 1 $aALFRADIQUE, V. A. P.
700 1 $aPINTO, P. H. N.
700 1 $aRIBEIRO, L. S.
700 1 $aOLIVEIRA, C. S.
700 1 $aSOUZA FABJAN, J. M. G. de
700 1 $aCAMARGO, L. S. de A.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca |
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Origem |
Tipo/Formato |
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Cutter |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
03/02/2020 |
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Data da última atualização: |
03/02/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
VASCONCELOS, J. F.; FROTA, G. A.; MONTEIRO, J. P.; VIEIRA, L. da S. |
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Afiliação: |
JANAELIA FERREIRA VASCONCELOS; GRACIELLE ARAÚJO FROTA; JOMAR PATRICIO MONTEIRO, CNPC; LUIZ DA SILVA VIEIRA, CNPC. |
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Título: |
Diagnóstico da resistência anti-helmíntica em rebanhos da Embrapa Caprinos e Ovinos. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA UNIVERSIDADE ESTADUAL VALE DO ACARAÚ, 19., 2017, Sobral. Conciência e paz; resumos. Sobral: Universidade Estadual Vale do Acaraú, 2017. |
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Idioma: |
Português |
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Conteúdo: |
O parasitismo por nematoides gastrointestinais é um dos fatores limitantes na criação de pequenos ruminantes. Dentre os parasitas que acometem esses animais destaca-se Haemonchus contortus por ser o mais prevalente e possuir alta patogenicidade e potencial biótico. O controle dos nematoides é realizado por meio de anti-helmínticos de amplo espectro de ação sendo os benzimidazois (BZs) os mais utilizados. No entanto, a utilização desses fármacos leva ao desenvolvimento de resistência anti-helmíntica, sendo caracterizada quando os parasitos resistem a doses do fármaco que normalmente seria eficaz. Para o monitoramento dessa resistência, testes fenotípicos que apresentam baixa sensibilidade são realizados. Dessa forma, testes mais sensíveis como os moleculares baseados em detecção de Polimorfismo de Nucleotídeo Único (SNP) no gene do isotipo 1 da beta-tubulina em H. contortus através da PCR em tempo real (qPCR) são necessários para diagnosticar a resistência antes que sua incidência se torne grave. Diante disso este trabalho teve como objetivo determinar o grau de resistência em rebanhos da Embrapa Caprinos e Ovinos, através da análise fenotípica e molecular |
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Palavras-Chave: |
Resistance to anthelmintics. |
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Thesagro: |
Anti-Helmíntico; Caprino; Haemonchus Contortus; Ovino; Polimorfismo; Resistência. |
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Thesaurus NAL: |
Brazil; Drug resistance; Goats; Sheep; Small ruminants. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/210080/1/CNPC-2017-Diagnostico.pdf
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Marc: |
LEADER 02145nam a2200289 a 4500
001 2119719
005 2020-02-03
008 2017 bl uuuu u00u1 u #d
100 1 $aVASCONCELOS, J. F.
245 $aDiagnóstico da resistência anti-helmíntica em rebanhos da Embrapa Caprinos e Ovinos.$h[electronic resource]
260 $aIn: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA UNIVERSIDADE ESTADUAL VALE DO ACARAÚ, 19., 2017, Sobral. Conciência e paz; resumos. Sobral: Universidade Estadual Vale do Acaraú$c2017
520 $aO parasitismo por nematoides gastrointestinais é um dos fatores limitantes na criação de pequenos ruminantes. Dentre os parasitas que acometem esses animais destaca-se Haemonchus contortus por ser o mais prevalente e possuir alta patogenicidade e potencial biótico. O controle dos nematoides é realizado por meio de anti-helmínticos de amplo espectro de ação sendo os benzimidazois (BZs) os mais utilizados. No entanto, a utilização desses fármacos leva ao desenvolvimento de resistência anti-helmíntica, sendo caracterizada quando os parasitos resistem a doses do fármaco que normalmente seria eficaz. Para o monitoramento dessa resistência, testes fenotípicos que apresentam baixa sensibilidade são realizados. Dessa forma, testes mais sensíveis como os moleculares baseados em detecção de Polimorfismo de Nucleotídeo Único (SNP) no gene do isotipo 1 da beta-tubulina em H. contortus através da PCR em tempo real (qPCR) são necessários para diagnosticar a resistência antes que sua incidência se torne grave. Diante disso este trabalho teve como objetivo determinar o grau de resistência em rebanhos da Embrapa Caprinos e Ovinos, através da análise fenotípica e molecular
650 $aBrazil
650 $aDrug resistance
650 $aGoats
650 $aSheep
650 $aSmall ruminants
650 $aAnti-Helmíntico
650 $aCaprino
650 $aHaemonchus Contortus
650 $aOvino
650 $aPolimorfismo
650 $aResistência
653 $aResistance to anthelmintics
700 1 $aFROTA, G. A.
700 1 $aMONTEIRO, J. P.
700 1 $aVIEIRA, L. da S.
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Embrapa Caprinos e Ovinos (CNPC) |
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