| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
29/09/2017 |
|
Data da última atualização: |
24/11/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. |
|
Afiliação: |
Helena Fabiana Reis de Almeida Saraiva, Universidade Federal Fluminense (UFF) - Niteroi, RJ, Brazil; Ribrio Ivan Tavares Pereira Batista, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brasil; Vivian Angélico Pereira Alfradique, UFF - Niteroi, RJ, Brazil; Pedro Henrique Nicolau Pinto, UFF - Niteroi, RJ, Brazil; Lilian Santos Ribeiro, UFF - Niteroi, RJ, Brazil; CLARA SLADE OLIVEIRA, CNPGL; Joanna Maria Gonçalves de Souza-Fabjan, UFF - Niteroi, RJ, Brazil; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Zandonadi Brandão, UFF - Niteroi, RJ, Brazil. |
|
Título: |
l-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Theriogenology, v. 105, p. 150-157, Jan. 2018. |
|
DOI: |
https://doi.org/10.1016/j.theriogenology.2017.09.022 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: L-carnitine is an antioxidant and beta-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies. MenosAbstract: L-carnitine is an antioxidant and beta-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control... Mostrar Tudo |
|
Palavras-Chave: |
Animal embryos; ATP cycle; Drug effects; Mitochondrial genetics; Santa ines; Survival. |
|
Thesagro: |
Ovino. |
|
Thesaurus Nal: |
Carnitine; Cell culture; Cryopreservation; Ewes; Gene expression; In vitro culture; oocytes; Sheep; Vitrification. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 03336naa a2200433 a 4500
001 2076472
005 2022-11-24
008 2017 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2017.09.022$2DOI
100 1 $aSARAIVA, H. F. R. de A.
245 $al-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression.$h[electronic resource]
260 $c2017
520 $aAbstract: L-carnitine is an antioxidant and beta-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies.
650 $aCarnitine
650 $aCell culture
650 $aCryopreservation
650 $aEwes
650 $aGene expression
650 $aIn vitro culture
650 $aoocytes
650 $aSheep
650 $aVitrification
650 $aOvino
653 $aAnimal embryos
653 $aATP cycle
653 $aDrug effects
653 $aMitochondrial genetics
653 $aSanta ines
653 $aSurvival
700 1 $aBATISTA, R. I. T. P.
700 1 $aALFRADIQUE, V. A. P.
700 1 $aPINTO, P. H. N.
700 1 $aRIBEIRO, L. S.
700 1 $aOLIVEIRA, C. S.
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aCAMARGO, L. S. de A.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
773 $tTheriogenology$gv. 105, p. 150-157, Jan. 2018.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Semiárido. |
|
Data corrente: |
30/11/2001 |
|
Data da última atualização: |
21/11/2018 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso |
|
Autoria: |
HENRIQUES, L. T.; SILVA, J. F. C. da; ESTRADA, L. H. C.; VASQUEZ, H. M.; ARAUJO, G. G. L. de; SCARDUA, S. S.; BARROS, E. E. L. de. |
|
Afiliação: |
GHERMAN GARCIA LEAL DE ARAUJO, CPATSA. |
|
Título: |
pH e amonia ruminais de bovinos alimentados com silagens de capim-elefante e de milho adicionadas de acipin. |
|
Ano de publicação: |
2001 |
|
Fonte/Imprenta: |
In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 38., 2001, Piracicaba. Anais... Piracicaba: FEALQ, 2001. |
|
Páginas: |
p. 1322-1323. |
|
Idioma: |
Português |
|
Conteúdo: |
Avaliou-se o comportamento do pH e amonia ruminais de bovinos machos castrados mesticos Holandes x Zebu, alimentados com silagens de capim-Elefante e de milho adicionadas de acipin. O pH ruminal foi maior quando os animais receberam silagem de capim Elefante, e sofreu influencia do tempo apos alimentacao. As concentracoes de amonia ruminal tambem foram superiores nos animais que receberam silagem de capim-Elefante, e apresentaram um comportamento quadratico em funcao dos horarios de amostragem. |
|
Palavras-Chave: |
Elephant grass; Liquido de rumen; Rumen fluid. |
|
Thesagro: |
Bovino; Capim Elefante; Digestibilidade; Fermentação; Milho; Silagem. |
|
Thesaurus NAL: |
cattle; corn; digestibility; fermentation; silage. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/186584/1/pH-e-amonia-ruminais-de-bovinos-alimentados-com-silagens...-v.38p1322-1323Class363-R444a.pdf
|
|
Marc: |
LEADER 01537nam a2200361 a 4500
001 1134442
005 2018-11-21
008 2001 bl uuuu u00u1 u #d
100 1 $aHENRIQUES, L. T.
245 $apH e amonia ruminais de bovinos alimentados com silagens de capim-elefante e de milho adicionadas de acipin.
260 $aIn: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 38., 2001, Piracicaba. Anais... Piracicaba: FEALQ$c2001
300 $ap. 1322-1323.
520 $aAvaliou-se o comportamento do pH e amonia ruminais de bovinos machos castrados mesticos Holandes x Zebu, alimentados com silagens de capim-Elefante e de milho adicionadas de acipin. O pH ruminal foi maior quando os animais receberam silagem de capim Elefante, e sofreu influencia do tempo apos alimentacao. As concentracoes de amonia ruminal tambem foram superiores nos animais que receberam silagem de capim-Elefante, e apresentaram um comportamento quadratico em funcao dos horarios de amostragem.
650 $acattle
650 $acorn
650 $adigestibility
650 $afermentation
650 $asilage
650 $aBovino
650 $aCapim Elefante
650 $aDigestibilidade
650 $aFermentação
650 $aMilho
650 $aSilagem
653 $aElephant grass
653 $aLiquido de rumen
653 $aRumen fluid
700 1 $aSILVA, J. F. C. da
700 1 $aESTRADA, L. H. C.
700 1 $aVASQUEZ, H. M.
700 1 $aARAUJO, G. G. L. de
700 1 $aSCARDUA, S. S.
700 1 $aBARROS, E. E. L. de
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Semiárido (CPATSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
