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| 1. |  | ALBUQUERQUE, S. S. C.; LIRA, M. A.; SANTOS, M. V. F. dos; DUBEUX JÚNIOR, J. C. B.; MELO, J. N. de; FARIAS, I. Disponibilidade de forragem e composição botânica de uma pastagem após períodos de diferimento. I In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL 2.; SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 8., 2000, Teresina. Resumos... Teresina: Sociedade Nordestina de Produção Animal, 2000. v. 2. p. 66-68.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
14/06/2010 |
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Data da última atualização: |
01/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
NOBILE, P. M.; QUECINI, V.; BAZZO, B.; QUITERIO, G.; MAZZAFERA, P.; COLOMBO, C. A. |
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Afiliação: |
PAULA M. NOBILE, IAC; VERA MARIA QUECINI, CNPUV; BARBARA BAZZO, IAC; GABRIELA QUITERIO, IAC; PAULO MAZZAFERA, INSTITUTO DE BIOLOGIA - UNICAMP; CARLOS A. COLOMBO, IAC. |
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Título: |
Transcriptional profile of genes involved in the byosynthesis of phytate and ferritin in coffea. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Journal of Agricultural and Food Chemistry, Washington DC, v. 58, n. 6, p. 3479-3487, mar. 2010. |
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DOI: |
10.1021/jf9043088 |
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Idioma: |
Inglês |
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Conteúdo: |
The present work aimed to study the control of the biosynthesis of the antinutritional factor phytate and its associated Fe-rich protein family, ferritin, in coffee. Phytate has the ability to chelate Fe, making it unavailable to human absorption. The Coffea genome databases were queried for genes associated with phytate metabolism and ferritin genes. The genetic framework for phytate biosynthesis and its reverse pathway was identified in silico analyses and indicate that Coffea phosphatidyl inositol kinase and monophosphatase families play nonredundant roles in phytate metabolism. The transcriptional profiles of phytate biosynthesis key-genes MYO-INOSITOL(3)P1 SYNTHASE, two genes coding for PHOSPHATIDYL INOSITOL KINASE, and three FERRITIN genes were temporally evaluated by qPCR in coffee seeds from two crop locations, Adamantina-SP and Ouro-Fino-MG, the last one traditionally associated with high-quality coffee beverage grain. A targeted metabolome profile of phytic acid contents throughout three fruit maturation stages in association with the transcriptional analysis was also obtained. Taken together, our data indicate that the investigated local conditions did not cause significant alterations in phytate biosynthesis. Futhermore, the temporal transcriptional profiling revealed that candidate gene expression is regulated independently of phytate accumulation. In contrast, the expression profile of ferritin-unit genes is affected by environmental conditions and genetic background. The roles of the investigated genes are discussed concerning the quality of coffee beverage. MenosThe present work aimed to study the control of the biosynthesis of the antinutritional factor phytate and its associated Fe-rich protein family, ferritin, in coffee. Phytate has the ability to chelate Fe, making it unavailable to human absorption. The Coffea genome databases were queried for genes associated with phytate metabolism and ferritin genes. The genetic framework for phytate biosynthesis and its reverse pathway was identified in silico analyses and indicate that Coffea phosphatidyl inositol kinase and monophosphatase families play nonredundant roles in phytate metabolism. The transcriptional profiles of phytate biosynthesis key-genes MYO-INOSITOL(3)P1 SYNTHASE, two genes coding for PHOSPHATIDYL INOSITOL KINASE, and three FERRITIN genes were temporally evaluated by qPCR in coffee seeds from two crop locations, Adamantina-SP and Ouro-Fino-MG, the last one traditionally associated with high-quality coffee beverage grain. A targeted metabolome profile of phytic acid contents throughout three fruit maturation stages in association with the transcriptional analysis was also obtained. Taken together, our data indicate that the investigated local conditions did not cause significant alterations in phytate biosynthesis. Futhermore, the temporal transcriptional profiling revealed that candidate gene expression is regulated independently of phytate accumulation. In contrast, the expression profile of ferritin-unit genes is affected by environmental conditions and genetic back... Mostrar Tudo |
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Palavras-Chave: |
Fator anti-nutricional; Ferritina; Fitato. |
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Thesagro: |
Biologia; Café; Ferro; Genética. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204172/1/12303-2010-p.3479-3487-2.pdf
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Marc: |
LEADER 02404naa a2200277 a 4500
001 1854944
005 2019-11-01
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1021/jf9043088$2DOI
100 1 $aNOBILE, P. M.
245 $aTranscriptional profile of genes involved in the byosynthesis of phytate and ferritin in coffea.$h[electronic resource]
260 $c2010
520 $aThe present work aimed to study the control of the biosynthesis of the antinutritional factor phytate and its associated Fe-rich protein family, ferritin, in coffee. Phytate has the ability to chelate Fe, making it unavailable to human absorption. The Coffea genome databases were queried for genes associated with phytate metabolism and ferritin genes. The genetic framework for phytate biosynthesis and its reverse pathway was identified in silico analyses and indicate that Coffea phosphatidyl inositol kinase and monophosphatase families play nonredundant roles in phytate metabolism. The transcriptional profiles of phytate biosynthesis key-genes MYO-INOSITOL(3)P1 SYNTHASE, two genes coding for PHOSPHATIDYL INOSITOL KINASE, and three FERRITIN genes were temporally evaluated by qPCR in coffee seeds from two crop locations, Adamantina-SP and Ouro-Fino-MG, the last one traditionally associated with high-quality coffee beverage grain. A targeted metabolome profile of phytic acid contents throughout three fruit maturation stages in association with the transcriptional analysis was also obtained. Taken together, our data indicate that the investigated local conditions did not cause significant alterations in phytate biosynthesis. Futhermore, the temporal transcriptional profiling revealed that candidate gene expression is regulated independently of phytate accumulation. In contrast, the expression profile of ferritin-unit genes is affected by environmental conditions and genetic background. The roles of the investigated genes are discussed concerning the quality of coffee beverage.
650 $aBiologia
650 $aCafé
650 $aFerro
650 $aGenética
653 $aFator anti-nutricional
653 $aFerritina
653 $aFitato
700 1 $aQUECINI, V.
700 1 $aBAZZO, B.
700 1 $aQUITERIO, G.
700 1 $aMAZZAFERA, P.
700 1 $aCOLOMBO, C. A.
773 $tJournal of Agricultural and Food Chemistry, Washington DC$gv. 58, n. 6, p. 3479-3487, mar. 2010.
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Embrapa Uva e Vinho (CNPUV) |
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