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| Registros recuperados : 98 | |
| 25. |  | OLIVEIRA, E. L de; GUIMARÃES, V. P.; ALBUQUERQUE, F. H. M. A. R. de; HOLANDA JÚNIOR, E. V. Study the impact of mortality in Saanen and Nubian herds. In: INTERNATIONAL CONFERENCE ON GOATS, 9.; REUNIÓN NACIONAL SOBRE CAPRINOCULTURA, 23., 2008, Querétaro, México. Sustainable goat production: challenges and opportunities of small and large enterprises; proceedings. Querétaro: International Goat Association, 2008. p. 262. Abstract 383.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 27. | | GUIMARÃES, V. P.; HOLANDA JUNIOR, E. V.; ALBUQUERQUE, F. H. M. A. R. de; OLIVEIRA, L. S. Development of a mathematical model to study the impacts of production and management policie on the dynamics of a dairy goat herd in the Northeast of Brazil. In: INTERNATIONAL CONFERENCE ON GOATS, 9.; REUNIÓN NACIONAL SOBRE CAPRINOCULTURA, 23., 2008, Querétaro, México. Sustainable goat production: challenges and opportunities of small and large enterprises; proceedings. Querétaro: International Goat Association, 2008. p. 134 ref. 142.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 31. | | GUIMARÃES, V. P.; HOLANDA JÚNIOR, E. V.; OLIVEIRA, E. L de; ALBUQUERQUE, F. H. M. A. R. de. Impact of puberty and lactation order of a dairy goat herd in Northeast of Brazil. In: INTERNATIONAL CONFERENCE ON GOATS, 9.; REUNIÓN NACIONAL SOBRE CAPRINOCULTURA, 23., 2008, Querétaro, México. Sustainable goat production: challenges and opportunities of small and large enterprises; proceedings. Querétaro: International Goat Association, 2008. p. 134 ref. 143.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 32. | | ALBUQUERQUE, F. H. M. A. R. de; GUIMARÃES, V. P.; OLIVEIRA, L. S.; HOLANDA JUNIOR, E. V. Impact of the service period under the dynamic of a semi-intensive dairy goat herd. In: INTERNATIONAL CONFERENCE ON GOATS, 9.; REUNIÓN NACIONAL SOBRE CAPRINOCULTURA, 23., 2008, Querétaro, México. Sustainable goat production: challenges and opportunities of small and large enterprises; proceedings. Querétaro: International Goat Association, 2008. p. 120. ref. 115.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 33. | | ARAÚJO, M. S. de; JÚLIO, M. R. F.; GUIMARÃES, V. P.; FARIAS, J. L. de S.; OLIVEIRA, L. S.; ALBUQUERQUE, F. H. M. A. R. de. As principais alternativas alimentares utilizadas por produtores familiares na dieta de caprinos e ovinos mo município de Quixadá - CE. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA UNIVERSIDADE ESTADUAL VALE DO ACARAÚ, 7., 2010, Sobral. Iniciação para o semiárido: [Resumos]. Sobral: Universidade Estadual Vale do Acaraú, 2010. 1 CD ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 38. | | GONÇALVES, J. de L.; BOMFIM, M. A. D.; TEDESCHI, L. O.; GALVANI, D. B.; SOUSA, R. T. de; ALBUQUERQUE, F. H. M. A. R. de. Balanços de energia e proteína metabolizável de ovelhas em pastagem de capim-tanzânia. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 8., 2013, Fortaleza. [Anais...]. [Sobral: Universidade Estadual Vale do Acaraú; Embrapa Caprinos e Ovinos, 2013]. 4 f.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 39. | | ABREU, U. G. P. de; LIMA, L. D. de; ALBUQUERQUE, F. H. M. A. R. de; SOUZA, J. D. F. de; GOMES, E. G. Avaliação da eficiência de produtores do semiárido, por meio de análise envoltória de dados (DEA) com restrições aos pesos. In: SIMPÓSIO BRASILEIRO DE AGROPECUÁRIA SUSTENTÁVEL., 9.; CONGRESSO INTERNACIONAL DE AGROPECUÁRIA SUSTENTÁVEL, 6. Viçosa. Ciência, tecnologia e inovação para o desenvolvimento agropecuário sustentável: anais...Viçosa: UFV, 2018. Não paginado. SIMBRAS.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Pantanal. |
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| 40. | | MATOS, A. F. M.; SANTOS, T. L. B. A.; FONTENELE, J. A.; SIEBRA, P. S. C.; ALBUQUERQUE, F. H. M. A. R. de; TEIXEIRA, M. Avaliação hematológica de ovinos imunizados com glicoproteina instestinal de Haemonchus contortus. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 3., 2014, Sobral. Anais... Sobral: Embrapa Caprinos e Ovinos, 2014. p. 39.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 98 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Soja. |
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Data corrente: |
02/10/2015 |
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Data da última atualização: |
13/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; REIS, R. R.; FARIAS, J. R. B.; HARMON, F. G.; MOLINARI, H. B. C.; MOLINARI, M. D. C.; NEPOMUCENO, A. L. |
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Afiliação: |
UEL; AUTOR; AUTOR; UFV; UEL; JOSE RENATO BOUCAS FARIAS, CNPSO; U C BERKELEY - BERKELEY; HUGO BRUNO CORREA MOLINARI, CNPAE; UEL; ALEXANDRE LIMA NEPOMUCENO, CNPSO. |
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Título: |
Transcriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plos One, v. 10, n. 9, e0139051, 2015. |
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Páginas: |
16 p. |
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DOI: |
10.1371/journal.pone.0139051 |
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Idioma: |
Inglês |
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Conteúdo: |
The soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day. MenosThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean ge... Mostrar Tudo |
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Palavras-Chave: |
Drought stress; Soybean. |
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Thesagro: |
Estiagem; Resistência à seca; Seca; Soja. |
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Thesaurus NAL: |
Drought tolerance; Soybeans; Transcriptome. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166647/1/nepomuceno-plos-one.pdf
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Marc: |
LEADER 02592naa a2200361 a 4500
001 2079599
005 2017-11-13
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0139051$2DOI
100 1 $aMARCOLINO-GOMES, J.
245 $aTranscriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day.$h[electronic resource]
260 $c2015
300 $a16 p.
520 $aThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day.
650 $aDrought tolerance
650 $aSoybeans
650 $aTranscriptome
650 $aEstiagem
650 $aResistência à seca
650 $aSeca
650 $aSoja
653 $aDrought stress
653 $aSoybean
700 1 $aRODRIGUES, F. A.
700 1 $aFUGANTI-PAGLIARINI, R.
700 1 $aNAKAYAMA, T. J.
700 1 $aREIS, R. R.
700 1 $aFARIAS, J. R. B.
700 1 $aHARMON, F. G.
700 1 $aMOLINARI, H. B. C.
700 1 $aMOLINARI, M. D. C.
700 1 $aNEPOMUCENO, A. L.
773 $tPlos One$gv. 10, n. 9, e0139051, 2015.
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Registro original: |
Embrapa Soja (CNPSO) |
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