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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
05/04/2010 |
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Data da última atualização: |
09/04/2010 |
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Autoria: |
SIMPSON, R. J.; ADAMS, P. D.; GOLEMIS, E. A. (ed.). |
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Título: |
Basic methods in protein purification and analysis. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
New York: Cold Spring Harbor, 2009. |
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Páginas: |
436 p. |
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ISBN: |
978-0-87969-868-3 |
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Idioma: |
Inglês |
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Conteúdo: |
Introduction to protein purification strategies; Preparation of cellular and subcellular extracts. SECTION 1: PURIFICATION USING GEL ELECTROPHORESIS - One - dimensional polyacrylamide gel electrophoresis; Preparative two-dimensional gel electrophoresis with immobilized pH gradients. SECTION 2: PURIFICATION USING COLUMN CHROMATOGRAPHY - Ion-exchange Chromatography; Size-exclusion Chromatografy; Reversed-phase High-performance Liquid Chromatography; Affinity and Immunoaffinity Chromatography; Metal chelate affinity Chromatography; Multidimensional Chromatography of infact proteins. SECTION 3: CHARACTERIZING PROTEIN COMPLEXES - Identification of protein - protein Interactions by Conventional Column Chromatografy; Identification of Associeated Proteins by Coimmunoprecipitation; Chromatin Immunoprecipitacion of Protein Complexes; Identification of Protein Interactions with Glutathione-S-Transferase Fusion Proteins; Direct Visualizacion of protein Interations in living cells using bimolecular fluorescence complementation analysis; In silico tools: Analysis for Creating focused interaction networks. |
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Palavras-Chave: |
Análise de proteína - Método; Cromatografia - Método; Eletroforese - Método; Imunoprecipitação - Método; Manual de laboratório; Proteína - Purificação. |
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Categoria do assunto: |
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Marc: |
LEADER 01769nam a2200229 a 4500
001 1663160
005 2010-04-09
008 2009 bl uuuu 00u1 u #d
020 $a978-0-87969-868-3
100 1 $aSIMPSON, R. J.
245 $aBasic methods in protein purification and analysis.
260 $aNew York: Cold Spring Harbor$c2009
300 $a436 p.
520 $aIntroduction to protein purification strategies; Preparation of cellular and subcellular extracts. SECTION 1: PURIFICATION USING GEL ELECTROPHORESIS - One - dimensional polyacrylamide gel electrophoresis; Preparative two-dimensional gel electrophoresis with immobilized pH gradients. SECTION 2: PURIFICATION USING COLUMN CHROMATOGRAPHY - Ion-exchange Chromatography; Size-exclusion Chromatografy; Reversed-phase High-performance Liquid Chromatography; Affinity and Immunoaffinity Chromatography; Metal chelate affinity Chromatography; Multidimensional Chromatography of infact proteins. SECTION 3: CHARACTERIZING PROTEIN COMPLEXES - Identification of protein - protein Interactions by Conventional Column Chromatografy; Identification of Associeated Proteins by Coimmunoprecipitation; Chromatin Immunoprecipitacion of Protein Complexes; Identification of Protein Interactions with Glutathione-S-Transferase Fusion Proteins; Direct Visualizacion of protein Interations in living cells using bimolecular fluorescence complementation analysis; In silico tools: Analysis for Creating focused interaction networks.
653 $aAnálise de proteína - Método
653 $aCromatografia - Método
653 $aEletroforese - Método
653 $aImunoprecipitação - Método
653 $aManual de laboratório
653 $aProteína - Purificação
700 1 $aADAMS, P. D.
700 1 $aGOLEMIS, E. A.
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Registro original: |
Embrapa Agroindústria Tropical (CNPAT) |
