Data/hora: 05/06/2024 09:02:37

Biblioteca(s): 	Embrapa Agroindústria Tropical.	
Data corrente: 	05/04/2010	
Data da última atualização: 	09/04/2010	
Autoria: 	SIMPSON, R. J.; ADAMS, P. D.; GOLEMIS, E. A. (ed.).	
Título: 	Basic methods in protein purification and analysis.	
Ano de publicação: 	2009	
Fonte/Imprenta: 	New York: Cold Spring Harbor, 2009.	
Páginas: 	436 p.	
ISBN: 	978-0-87969-868-3	
Idioma: 	Inglês	
Conteúdo: 	Introduction to protein purification strategies; Preparation of cellular and subcellular extracts. SECTION 1: PURIFICATION USING GEL ELECTROPHORESIS - One - dimensional polyacrylamide gel electrophoresis; Preparative two-dimensional gel electrophoresis with immobilized pH gradients. SECTION 2: PURIFICATION USING COLUMN CHROMATOGRAPHY - Ion-exchange Chromatography; Size-exclusion Chromatografy; Reversed-phase High-performance Liquid Chromatography; Affinity and Immunoaffinity Chromatography; Metal chelate affinity Chromatography; Multidimensional Chromatography of infact proteins. SECTION 3: CHARACTERIZING PROTEIN COMPLEXES - Identification of protein - protein Interactions by Conventional Column Chromatografy; Identification of Associeated Proteins by Coimmunoprecipitation; Chromatin Immunoprecipitacion of Protein Complexes; Identification of Protein Interactions with Glutathione-S-Transferase Fusion Proteins; Direct Visualizacion of protein Interations in living cells using bimolecular fluorescence complementation analysis; In silico tools: Analysis for Creating focused interaction networks.	
Palavras-Chave: 	Análise de proteína - Método; Cromatografia - Método; Eletroforese - Método; Imunoprecipitação - Método; Manual de laboratório; Proteína - Purificação.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Agroindústria Tropical (CNPAT)	
Exemplares: 	CNPAT - (11934-1) - ADC - LV - PP - 572.6 - S613b - 2010.03397 - DIS