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Registro Completo |
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
13/07/2011 |
Data da última atualização: |
20/03/2012 |
Tipo da produção científica: |
Circular Técnica |
Autoria: |
ABDELNUR, P. V. |
Afiliação: |
PATRICIA VERARDI ABDELNUR, CNPAE. |
Título: |
Imageamento químico por espectrometria de massas utilizando MALDI (MALDI Imaging Mass Spectrometry) aplicado a tecidos vegetais. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Brasília, DF: Embrapa Agroenergia, 2011.. |
Páginas: |
6 p. |
Série: |
(Embrapa Agroenergia. Circular técnica, 006). |
ISSN: |
2177-4420 |
Idioma: |
Português |
Palavras-Chave: |
Espectrometria de massas; Imageamento químico; Ionização por MALDI; MALDI-IMS. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/37368/1/CITE-06.pdf
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Marc: |
LEADER 00609nam a2200181 a 4500 001 1895823 005 2012-03-20 008 2011 bl uuuu u0uu1 u #d 022 $a2177-4420 100 1 $aABDELNUR, P. V. 245 $aImageamento químico por espectrometria de massas utilizando MALDI (MALDI Imaging Mass Spectrometry) aplicado a tecidos vegetais. 260 $aBrasília, DF: Embrapa Agroenergia$c2011 300 $a6 p. 490 $a(Embrapa Agroenergia. Circular técnica, 006). 653 $aEspectrometria de massas 653 $aImageamento químico 653 $aIonização por MALDI 653 $aMALDI-IMS
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Suínos e Aves. |
Data corrente: |
16/03/2020 |
Data da última atualização: |
20/04/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
OKINO, C. H.; RECH, D. V.; JAENISCH, F. R. F.; TREVISOL, I. M.; REBELATTO, R.; COLDEBELLA, A.; MORES, M. A. Z.; GIGLIOTI, R.; VAZ, C. S. L. |
Afiliação: |
CINTIA HIROMI OKINO, CPPSE; DAIANE VOSS RECH, CNPSA; FATIMA REGINA FERREIRA JAENISCH, CNPSA; IARA MARIA TREVISOL, CNPSA; RAQUEL REBELATTO, CNPSA; ARLEI COLDEBELLA, CNPSA; MARCOS ANTONIO ZANELLA MORES, CNPSA; RODRIGO GIGLIOTI, UNESP; CLARISSA SILVEIRA LUIZ VAZ, CNPSA. |
Título: |
Detecting infectious bursal disease using a VP1 gene-based RT-qPCR assay compared to standard methods of virus isolation, ELISA, and histopathology. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Current Microbiology, 2020. |
Páginas: |
8 p. |
ISSN: |
1432-0991 |
DOI: |
https://doi.org/10.1007/s00284-020-01906-7 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Infectious bursal disease (IBD) is an immunosuppressive viral disease of chickens, associated with severe economic losses and major threats to poultry production worldwide. Disease prevention programs rely on unequivocal identification of the pathogen, as well as vaccination programs. This study developed a sensitive, one-step, real-time, quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay using a hydrolysis probe system for infectious bursal disease virus (IBDV, VP1 gene) detection and quantification, which was compared to other routinely used diagnostic methods. The assay successfully detected IBD reference viruses and field isolates. The absence of cross-reactivity was detected with negative samples or with other avian viruses in the analytical specificity test. The detection limit of this assay was 70 RNA copies. RT-qPCR was more sensitive in the detection of serially diluted IBDV isolates compared to virus isolation. For clinical samples, the sensitivity and specificity values of RT-qPCR compared to enzyme-linked immunosorbent assay (ELISA) were 97.5% and 100%, respectively, and compared to histopathology, these values were 100% and 93.94%, respectively. RT-qPCR can provide a simple and reliable assay for IBDV surveillance programs and for evaluation of control strategies. |
Palavras-Chave: |
Doença infecciosa da bolsa (DII); Doença viral imunossupressora; Immunosuppressive viral disease of chickens; RT-qPCR. |
Thesagro: |
Avicultura; Elisa; Histopatologia; Produção Animal; Sanidade Animal; Virologia. |
Thesaurus NAL: |
Chickens; Infectious bursal disease. |
Categoria do assunto: |
-- H Saúde e Patologia |
Marc: |
LEADER 02532naa a2200397 a 4500 001 2121266 005 2020-04-20 008 2020 bl uuuu u00u1 u #d 022 $a1432-0991 024 7 $ahttps://doi.org/10.1007/s00284-020-01906-7$2DOI 100 1 $aOKINO, C. H. 245 $aDetecting infectious bursal disease using a VP1 gene-based RT-qPCR assay compared to standard methods of virus isolation, ELISA, and histopathology.$h[electronic resource] 260 $c2020 300 $a8 p. 520 $aAbstract: Infectious bursal disease (IBD) is an immunosuppressive viral disease of chickens, associated with severe economic losses and major threats to poultry production worldwide. Disease prevention programs rely on unequivocal identification of the pathogen, as well as vaccination programs. This study developed a sensitive, one-step, real-time, quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay using a hydrolysis probe system for infectious bursal disease virus (IBDV, VP1 gene) detection and quantification, which was compared to other routinely used diagnostic methods. The assay successfully detected IBD reference viruses and field isolates. The absence of cross-reactivity was detected with negative samples or with other avian viruses in the analytical specificity test. The detection limit of this assay was 70 RNA copies. RT-qPCR was more sensitive in the detection of serially diluted IBDV isolates compared to virus isolation. For clinical samples, the sensitivity and specificity values of RT-qPCR compared to enzyme-linked immunosorbent assay (ELISA) were 97.5% and 100%, respectively, and compared to histopathology, these values were 100% and 93.94%, respectively. RT-qPCR can provide a simple and reliable assay for IBDV surveillance programs and for evaluation of control strategies. 650 $aChickens 650 $aInfectious bursal disease 650 $aAvicultura 650 $aElisa 650 $aHistopatologia 650 $aProdução Animal 650 $aSanidade Animal 650 $aVirologia 653 $aDoença infecciosa da bolsa (DII) 653 $aDoença viral imunossupressora 653 $aImmunosuppressive viral disease of chickens 653 $aRT-qPCR 700 1 $aRECH, D. V. 700 1 $aJAENISCH, F. R. F. 700 1 $aTREVISOL, I. M. 700 1 $aREBELATTO, R. 700 1 $aCOLDEBELLA, A. 700 1 $aMORES, M. A. Z. 700 1 $aGIGLIOTI, R. 700 1 $aVAZ, C. S. L. 773 $tCurrent Microbiology, 2020.
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