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Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
25/04/2007 |
Data da última atualização: |
27/04/2007 |
Autoria: |
MORTEL, M. van de; RECKNOR, J. C.; NETLETON, D. S.; GODOY, C. V.; ABDELNOOR, R. V.; ALMEIDA, A. M. R.; BAUM, T. J.; WHITHAM, S. A. |
Título: |
Molecular characterization of the Asian soybean rust disease in resistant and susceptible soybean lines. |
Ano de publicação: |
2006 |
Fonte/Imprenta: |
In: NATIONAL SOYBEAN RUST SYMPOSIUM, 2006, St. Louis. Poster abstracts. [S.l.]: APS, 2006. |
Páginas: |
p. 6. |
Idioma: |
Inglês |
Notas: |
Poster 7. |
Conteúdo: |
Our goal is to acquire critical molecular data on the Asian soybean rust (ASR) infection process in susceptible and resistant soybean cultivars. To define specific molecular events in the soybean plant that are associated with ASR resistance and susceptibility, we detected gene expression over a 7-day time course in both ASR- and mock-infectecd leaves from susceptible and resistant (Rpp2) cultivars by using Affymetrix Soybean GeneChip microarrays. Statistical analysis showed that l ,516 and 894 genes in the susceptible and resistant cultivars, respectively, significantly changed expression. Most genes were differentially expressed within the first 3 days after infection (dai), followed by a 2-day time period in which very few genes were differentially exprcssed. After 4 dai, host gene expression diverged again between mock- and ASR-infected leaves. Arabidopsis homologs were identified for 1,302 and 773 differentially regulated genes in the susceptible and resistant cultivars, respectively, which allowed us to perform functional classification. These analyses revealed that genes with functions in metabolism and disease/defense were statistically overrepresented in both the susceptible and resistant cultivars gene lists. ASR-regulated genes involved in signal transduction and energy generation were overrepresented in the susceptible cultivar, while genes with functions in transport and transcription were overrepresented in the resistant cultivar. ASR-regulated genes involved in post-transcriptional regulation or with no known function were underrepresented in both cultivar?, and genes involved in cell structure, growth and development, and protein synthesis appeared to be underrepresented in the resistant cultivar. MenosOur goal is to acquire critical molecular data on the Asian soybean rust (ASR) infection process in susceptible and resistant soybean cultivars. To define specific molecular events in the soybean plant that are associated with ASR resistance and susceptibility, we detected gene expression over a 7-day time course in both ASR- and mock-infectecd leaves from susceptible and resistant (Rpp2) cultivars by using Affymetrix Soybean GeneChip microarrays. Statistical analysis showed that l ,516 and 894 genes in the susceptible and resistant cultivars, respectively, significantly changed expression. Most genes were differentially expressed within the first 3 days after infection (dai), followed by a 2-day time period in which very few genes were differentially exprcssed. After 4 dai, host gene expression diverged again between mock- and ASR-infected leaves. Arabidopsis homologs were identified for 1,302 and 773 differentially regulated genes in the susceptible and resistant cultivars, respectively, which allowed us to perform functional classification. These analyses revealed that genes with functions in metabolism and disease/defense were statistically overrepresented in both the susceptible and resistant cultivars gene lists. ASR-regulated genes involved in signal transduction and energy generation were overrepresented in the susceptible cultivar, while genes with functions in transport and transcription were overrepresented in the resistant cultivar. ASR-regulated genes involved ... Mostrar Tudo |
Categoria do assunto: |
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Marc: |
LEADER 02431naa a2200229 a 4500 001 1469976 005 2007-04-27 008 2006 bl uuuu u00u1 u #d 100 1 $aMORTEL, M. van de 245 $aMolecular characterization of the Asian soybean rust disease in resistant and susceptible soybean lines. 260 $c2006 300 $ap. 6. 500 $aPoster 7. 520 $aOur goal is to acquire critical molecular data on the Asian soybean rust (ASR) infection process in susceptible and resistant soybean cultivars. To define specific molecular events in the soybean plant that are associated with ASR resistance and susceptibility, we detected gene expression over a 7-day time course in both ASR- and mock-infectecd leaves from susceptible and resistant (Rpp2) cultivars by using Affymetrix Soybean GeneChip microarrays. Statistical analysis showed that l ,516 and 894 genes in the susceptible and resistant cultivars, respectively, significantly changed expression. Most genes were differentially expressed within the first 3 days after infection (dai), followed by a 2-day time period in which very few genes were differentially exprcssed. After 4 dai, host gene expression diverged again between mock- and ASR-infected leaves. Arabidopsis homologs were identified for 1,302 and 773 differentially regulated genes in the susceptible and resistant cultivars, respectively, which allowed us to perform functional classification. These analyses revealed that genes with functions in metabolism and disease/defense were statistically overrepresented in both the susceptible and resistant cultivars gene lists. ASR-regulated genes involved in signal transduction and energy generation were overrepresented in the susceptible cultivar, while genes with functions in transport and transcription were overrepresented in the resistant cultivar. ASR-regulated genes involved in post-transcriptional regulation or with no known function were underrepresented in both cultivar?, and genes involved in cell structure, growth and development, and protein synthesis appeared to be underrepresented in the resistant cultivar. 700 1 $aRECKNOR, J. C. 700 1 $aNETLETON, D. S. 700 1 $aGODOY, C. V. 700 1 $aABDELNOOR, R. V. 700 1 $aALMEIDA, A. M. R. 700 1 $aBAUM, T. J. 700 1 $aWHITHAM, S. A. 773 $tIn: NATIONAL SOYBEAN RUST SYMPOSIUM, 2006, St. Louis. Poster abstracts. [S.l.]: APS, 2006.
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Embrapa Soja (CNPSO) |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
06/12/2019 |
Data da última atualização: |
06/12/2019 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
VIDAL, L. de A.; ALMEIDA, J. D. de; PINTO, E. R. de C.; VEIGA, A. D.; FERNANDEZ, D.; GUERREIRO FILHO, O.; ALBUQUERQUE, E. V. S. de. |
Afiliação: |
LEONARDO DE AMORIM VIDAL, UNB; JULIANA DANTAS DE ALMEIDA, Cenargen; EDUARDO ROMANO DE CAMPOS PINTO, Cenargen; ADRIANO DELLY VEIGA, CPAC; DIANA FERNANDEZ, INSTITUT DE RECHERCHE POUR LE DÈVÈLOPPEMENT (IRD), FRANÇA; OLIVEIRO GUERREIRO FILHO, IAC; ERIKA VALERIA SALIBA ALBUQUERQUE FR, Cenargen. |
Título: |
Obtenção de RNA de bicho-mineiro (Leucoptera coffeella) para transcritômica e silenciamento gênico. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 10., 2019, Vitória, ES. Pesquisa, inovação e sustentabilidade dos cafés do Brasil. Brasília, DF: Embrapa Café, 2019. |
Idioma: |
Português |
Palavras-Chave: |
Coffee leaf miner; RNAi; Transcritoma; Transcritome. |
Thesagro: |
Leucoptera Coffeella; RNA. |
Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/206378/1/264-2481-1-PB.pdf
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Marc: |
LEADER 00858nam a2200241 a 4500 001 2116190 005 2019-12-06 008 2019 bl uuuu u00u1 u #d 100 1 $aVIDAL, L. de A. 245 $aObtenção de RNA de bicho-mineiro (Leucoptera coffeella) para transcritômica e silenciamento gênico.$h[electronic resource] 260 $aIn: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 10., 2019, Vitória, ES. Pesquisa, inovação e sustentabilidade dos cafés do Brasil. Brasília, DF: Embrapa Café$c2019 650 $aLeucoptera Coffeella 650 $aRNA 653 $aCoffee leaf miner 653 $aRNAi 653 $aTranscritoma 653 $aTranscritome 700 1 $aALMEIDA, J. D. de 700 1 $aPINTO, E. R. de C. 700 1 $aVEIGA, A. D. 700 1 $aFERNANDEZ, D. 700 1 $aGUERREIRO FILHO, O. 700 1 $aALBUQUERQUE, E. V. S. de
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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