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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
13/12/2004 |
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Data da última atualização: |
13/12/2004 |
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Autoria: |
ABDELNOOR, R. V. |
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Título: |
Cloning and characterization of MSH1 in higher plants and its involvment in regulation of substoichiometric shifting. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
2004. |
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Páginas: |
123 f. |
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Idioma: |
Inglês |
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Notas: |
Dissertation (Doctor of Phylosophy in Agronomy - Plant Breeding and Genetics) - University of Nebraska, Lincoln. |
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Conteúdo: |
Plant mitochondrial genomes are much larger and complex in structure than in other euraryotes. The high recombinational activity of these genomes produces sub-genomic molecules of different sizes that can be maintained in a variable copy number, a phenomenon that is called substoichiometriv shifting and is widespread in plants. The regulation of the copy number has been shown to be under nuclear control, but the mechanism of this regulation is still not well understood. CHM is a nuclear gene that influences the mitochondrial genome by modifying the relative copy number of some sub-genomic molecules in Arabidopsis. Mutations in this gene are characterized by green-white variegation, abnormal growth, distorted leaves and, insome cases cytoplasmic male sterility. The main objectives of this thesis are to map, clone and characterize the CHM locus in Arabidopsis and its orthologs in other plant species. The CHM mapping was done with F2 populations involving the mutant chm1-1. A candidate gene was identified, designated AtMSH1m which is homologous ti the MutS protein of E. coli that is involved in DNA mismatch repair. The identity of this gene was confirmed by T-DNA insertional mutagenesis and by sequencing the mutant alleles for the locus. To better understand the structure and function of MHS1 in plants, the full-length orthologous genes in rice, tomato, maize, soybean and common bean were isolated and sequenced. Multiple alignment and phylogenetic analysis allowed the postulation of the origin of this gene and the identification of specific domains that could be very important for their proper function in plant mitochondria, including an endonuclease domain on the c-terminus of MSH1. This endonuclease is unique to palnt MSH1 and may be responsible for some of the exclusive properties of this protein. Also, a model for the function of MSH1 in the regulation of substoichiometric shifting is presented. MenosPlant mitochondrial genomes are much larger and complex in structure than in other euraryotes. The high recombinational activity of these genomes produces sub-genomic molecules of different sizes that can be maintained in a variable copy number, a phenomenon that is called substoichiometriv shifting and is widespread in plants. The regulation of the copy number has been shown to be under nuclear control, but the mechanism of this regulation is still not well understood. CHM is a nuclear gene that influences the mitochondrial genome by modifying the relative copy number of some sub-genomic molecules in Arabidopsis. Mutations in this gene are characterized by green-white variegation, abnormal growth, distorted leaves and, insome cases cytoplasmic male sterility. The main objectives of this thesis are to map, clone and characterize the CHM locus in Arabidopsis and its orthologs in other plant species. The CHM mapping was done with F2 populations involving the mutant chm1-1. A candidate gene was identified, designated AtMSH1m which is homologous ti the MutS protein of E. coli that is involved in DNA mismatch repair. The identity of this gene was confirmed by T-DNA insertional mutagenesis and by sequencing the mutant alleles for the locus. To better understand the structure and function of MHS1 in plants, the full-length orthologous genes in rice, tomato, maize, soybean and common bean were isolated and sequenced. Multiple alignment and phylogenetic analysis allowed the postulat... Mostrar Tudo |
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LEADER 02416nam a2200133 a 4500
001 1467331
005 2004-12-13
008 2004 bl uuuu m 00u1 u #d
100 1 $aABDELNOOR, R. V.
245 $aCloning and characterization of MSH1 in higher plants and its involvment in regulation of substoichiometric shifting.
260 $a2004.$c2004
300 $a123 f.
500 $aDissertation (Doctor of Phylosophy in Agronomy - Plant Breeding and Genetics) - University of Nebraska, Lincoln.
520 $aPlant mitochondrial genomes are much larger and complex in structure than in other euraryotes. The high recombinational activity of these genomes produces sub-genomic molecules of different sizes that can be maintained in a variable copy number, a phenomenon that is called substoichiometriv shifting and is widespread in plants. The regulation of the copy number has been shown to be under nuclear control, but the mechanism of this regulation is still not well understood. CHM is a nuclear gene that influences the mitochondrial genome by modifying the relative copy number of some sub-genomic molecules in Arabidopsis. Mutations in this gene are characterized by green-white variegation, abnormal growth, distorted leaves and, insome cases cytoplasmic male sterility. The main objectives of this thesis are to map, clone and characterize the CHM locus in Arabidopsis and its orthologs in other plant species. The CHM mapping was done with F2 populations involving the mutant chm1-1. A candidate gene was identified, designated AtMSH1m which is homologous ti the MutS protein of E. coli that is involved in DNA mismatch repair. The identity of this gene was confirmed by T-DNA insertional mutagenesis and by sequencing the mutant alleles for the locus. To better understand the structure and function of MHS1 in plants, the full-length orthologous genes in rice, tomato, maize, soybean and common bean were isolated and sequenced. Multiple alignment and phylogenetic analysis allowed the postulation of the origin of this gene and the identification of specific domains that could be very important for their proper function in plant mitochondria, including an endonuclease domain on the c-terminus of MSH1. This endonuclease is unique to palnt MSH1 and may be responsible for some of the exclusive properties of this protein. Also, a model for the function of MSH1 in the regulation of substoichiometric shifting is presented.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 374 | |
| 5. |  | NEPOMUCENO, A. L.; ABDELNOOR, R.; CARNEIRO, N. A biotecnologia sul-americana na agricultura. In: CONGRESO DE SOJA DEL MERCOSUR, 3., 2006, Rosário. Mercosoja 2006: conferencias plenarias, foros, workshops. Rosário: Associación de la Cadena de Soja Argentina, 2006. p. 31-33. Nome correto do segundo autor ABDELNOOR, R. V.| Biblioteca(s): Embrapa Soja. |
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| 6. | | ABDELNOOR, R. V.; MORIYAMA, H.; MACKENZIE, S. Identification of Msh-1-homologue genes in soybean, rice, tomato and common bean. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 255. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
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| 14. | | CARDOSO, R.; CAITAR, V. L.; FERREIRA, E. C.; MARCELINO-GUIMARÃES, F. C.; ABDELNOOR, R. V. Associative mapping for bacterial pustule in soybean (Mapeamento associativo para pústula bacteriana em soja) in: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 51., 2019, Recife. Anais... Brasília, DF: SBF, 2019. p. 156.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 15. | | MORALES, A. M. A. P.; BORÉM, A.; GRAHAM, M.; ABDELNOOR, R. V. Advances on molecular studies of the interaction soybean - Asian rust. Crop Breeding Applied Biotechnology, Viçosa, MG, v. 12, n. 1, p. 1-7, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 16. | | SANTANA, R. H.; VERAS, H. C. T.; ABDELNOOR, R. V.; BARROS, L. M. G. Análise experimental da expressão diferencial de um transcrito de soja identificado através de northern eletrônico. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Resumo 011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | SILVA, J. F. V.; ABDELNOOR, R. V.; COSTA, C. L.; BAÍA, G. S. Análise de RAPD em isolados de Fusarium spp. parasitas de ovos de Heterodera glycines ou de plantas de soja. Fitopatologia Brasileira, Brasilia, DF, v. 25, n. 3, p. 491-496, set. 2000.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Soja. |
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| 18. | | LEMOS, N. G.; BRACCINI, A. de L.; ABDELNOOR, R. V.; SUENAGA, K.; YAMANAKA, N. Análise da resistência genética de linhagens de soja contendo genes de resistência para a ferrugem asiática piramidados. In: CONGRESO DE LA SOJA DEL MERCOSUR, 5.; FORO DE LA SOJA ASIA, 1., 2011, Rosário. Un grano: un universo. [Rosário: Asociación de la Cadena de la Soja Argentina], 2011. 4 p. 1 CD-ROM. MERCOSOJA 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 19. | | YAMANAKA, N.; HERCULANO, S. F. H.; ABDELNOOR, R. V.; NEPOMUCENO, A. L.; YORINORI, J. T. Avaliação de resistência a SDS utilizando isolado brasileiro em genótipos de soja chineses e japoneses resistentes a isolado argentino. In: CONGRESSO BRASILEIRO DE SOJA, 4., 2006, Londrina. Resumos... Londrina: Embrapa Soja, 2006. p. 104. Organizado por Odilon Ferreira Saraiva, Simone Ery Grosskopf.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 374 | |
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