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Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
18/02/2016 |
Data da última atualização: |
15/04/2016 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
MENDES, R.; ROMAGNOLI, E. M.; DUNLAP, C.; ABDALLA, A. L. |
Afiliação: |
RODRIGO MENDES, CNPMA; E. M. ROMAGNOLI; C. DUNLAP, National Center for Agricultural Utilization Research, Peoria, United States; A. L. ABDALLA, CENA/ESALQ USP. |
Título: |
Sugar cane bagasse affects bacterial community dynamics in the sheep rumen. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
In: SYMPOSIUM ON BACTERIAL GENETICS AND ECOLOGY, 13., 2015, Milan. The microbial continuity across changing ecosystems: proceedings... Milan: 2015. Ref. P SYM 5. |
Páginas: |
200 |
Idioma: |
Inglês |
Notas: |
13th BAGECO13 |
Conteúdo: |
Ruminants are herbivores and have evolved a symbiotic host-microbe relationship with a complex microbial community inhabiting the rumen allowing the use of lignocellulosic biomass as their main energy source. Considering that diet is one of the main drivers shaping the structure of the rumen microbiome, we investigated the impact of sugarcane bagasse in the rumen bacterial community dynamic using 16S rRNA (V3 and V6 regions) amplicon sequencing. We assessed three rumencannulated adult male sheep (Ovis aries) fed on a diet consisted of 30% concentrate and 70% roughage (control treatment) and three sheep fed on the same diet, but with 14% of the roughage portion replaced by sugarcane bagasse. Fluid and fiber were separately sampled 3 hours and 15, 30, 45, and 60 days after starting the experiment. Total genomic DNA was extracted from 60 independent samples (2 treatments X 3 replicates X 5 time points X 2 types of samples, i.e. fluid or fiber) for downstream analysis. The DNA was used as a template for amplification of V3 and V6 regions of the bacterial 16S rRNA gene and then sequenced using the PGMTM (Ion Torrent). Overall, the two dominant phyla were Bacteroidetes (42%) and Firmicutes (37%). The most abundant bacterial genus was Prevotella (20%), followed by Clostridium (9%), Ruminococcus (8%) and Butyrivibrio (2%). The principal coordinate analysis (PcoA) showed that the bacterial community was significantly different in both treatments at 60 days. Bacteroidales, Actinomycetales and Clostridiales were the top dynamic bacterial orders that significantly increased in relative abundance in the treatment with sugar cane bagasse after 60 days. Canonical correspondence analysis (CCA) revealed that the Clostridiales and Bacteroidales are positively correlated with propionate, butyrate, ammonia, and pH. These results indicate that a small replacement in the diet roughage portion influences de dynamic of specific bacterial taxa. This strategy can be used to reshape the bacterial community in the sheep rumen aiming to enrich the targeted bacterial taxa. Support FAPESP 2012/03848-8, 2012/24588-4 and 2014/00448-4. MenosRuminants are herbivores and have evolved a symbiotic host-microbe relationship with a complex microbial community inhabiting the rumen allowing the use of lignocellulosic biomass as their main energy source. Considering that diet is one of the main drivers shaping the structure of the rumen microbiome, we investigated the impact of sugarcane bagasse in the rumen bacterial community dynamic using 16S rRNA (V3 and V6 regions) amplicon sequencing. We assessed three rumencannulated adult male sheep (Ovis aries) fed on a diet consisted of 30% concentrate and 70% roughage (control treatment) and three sheep fed on the same diet, but with 14% of the roughage portion replaced by sugarcane bagasse. Fluid and fiber were separately sampled 3 hours and 15, 30, 45, and 60 days after starting the experiment. Total genomic DNA was extracted from 60 independent samples (2 treatments X 3 replicates X 5 time points X 2 types of samples, i.e. fluid or fiber) for downstream analysis. The DNA was used as a template for amplification of V3 and V6 regions of the bacterial 16S rRNA gene and then sequenced using the PGMTM (Ion Torrent). Overall, the two dominant phyla were Bacteroidetes (42%) and Firmicutes (37%). The most abundant bacterial genus was Prevotella (20%), followed by Clostridium (9%), Ruminococcus (8%) and Butyrivibrio (2%). The principal coordinate analysis (PcoA) showed that the bacterial community was significantly different in both treatments at 60 days. Bacteroidales, Actinomycet... Mostrar Tudo |
Thesagro: |
Bactéria; Bagaço; Cana de açúcar; Rúmen. |
Thesaurus Nal: |
Sugarcane bagasse. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139320/1/2015RA-007.pdf
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Marc: |
LEADER 02885nam a2200229 a 4500 001 2037517 005 2016-04-15 008 2015 bl uuuu u00u1 u #d 100 1 $aMENDES, R. 245 $aSugar cane bagasse affects bacterial community dynamics in the sheep rumen.$h[electronic resource] 260 $aIn: SYMPOSIUM ON BACTERIAL GENETICS AND ECOLOGY, 13., 2015, Milan. The microbial continuity across changing ecosystems: proceedings... Milan: 2015. Ref. P SYM 5.$c2015 300 $a200 500 $a13th BAGECO13 520 $aRuminants are herbivores and have evolved a symbiotic host-microbe relationship with a complex microbial community inhabiting the rumen allowing the use of lignocellulosic biomass as their main energy source. Considering that diet is one of the main drivers shaping the structure of the rumen microbiome, we investigated the impact of sugarcane bagasse in the rumen bacterial community dynamic using 16S rRNA (V3 and V6 regions) amplicon sequencing. We assessed three rumencannulated adult male sheep (Ovis aries) fed on a diet consisted of 30% concentrate and 70% roughage (control treatment) and three sheep fed on the same diet, but with 14% of the roughage portion replaced by sugarcane bagasse. Fluid and fiber were separately sampled 3 hours and 15, 30, 45, and 60 days after starting the experiment. Total genomic DNA was extracted from 60 independent samples (2 treatments X 3 replicates X 5 time points X 2 types of samples, i.e. fluid or fiber) for downstream analysis. The DNA was used as a template for amplification of V3 and V6 regions of the bacterial 16S rRNA gene and then sequenced using the PGMTM (Ion Torrent). Overall, the two dominant phyla were Bacteroidetes (42%) and Firmicutes (37%). The most abundant bacterial genus was Prevotella (20%), followed by Clostridium (9%), Ruminococcus (8%) and Butyrivibrio (2%). The principal coordinate analysis (PcoA) showed that the bacterial community was significantly different in both treatments at 60 days. Bacteroidales, Actinomycetales and Clostridiales were the top dynamic bacterial orders that significantly increased in relative abundance in the treatment with sugar cane bagasse after 60 days. Canonical correspondence analysis (CCA) revealed that the Clostridiales and Bacteroidales are positively correlated with propionate, butyrate, ammonia, and pH. These results indicate that a small replacement in the diet roughage portion influences de dynamic of specific bacterial taxa. This strategy can be used to reshape the bacterial community in the sheep rumen aiming to enrich the targeted bacterial taxa. Support FAPESP 2012/03848-8, 2012/24588-4 and 2014/00448-4. 650 $aSugarcane bagasse 650 $aBactéria 650 $aBagaço 650 $aCana de açúcar 650 $aRúmen 700 1 $aROMAGNOLI, E. M. 700 1 $aDUNLAP, C. 700 1 $aABDALLA, A. L.
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2. | | SCANAVACA JUNIOR, L. Água para todos. SOS Rios do Brasil, 20 mar. 2012. www.sosriosdobrasil.blogspot.com.brTipo: Artigo de Divulgação na Mídia |
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5. | | SCANAVACA JÚNIOR, L. Base teórica para o crédito de carbono. In: CONGRESSO BRASILEIRO SOBRE FLORESTAS ENERGÉTICAS, 1., 2009, Belo Horizonte. Anais. Colombo: Embrapa Florestas, 2009. 1 CD-ROM. (Embrapa Florestas. Documentos, 178). Seção: Sustentabilidade Ambiental, Social e Econômica.Biblioteca(s): Embrapa Florestas. |
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6. | | SCANAVACA JUNIOR, L. Base teórica para o crédito de carbono. In: CONGRESSO BRASILEIRO SOBRE FLORESTAS ENERGÉTICAS, 1., 2009, Belo Horizonte. Anais. Colombo: Embrapa Florestas, 2009. 1 CD-ROM. 4 p. (Embrapa Florestas. Documentos, 178).Tipo: Artigo em Anais de Congresso |
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