Data/hora: 02/06/2024 02:16:44

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 28/07/1995
Data da última atualização: | 09/11/1995
Autoria: | ESSERS, A. J. A.
Afiliação: | Department of Food Science. Wageningen Agricultural University, Bomenweg 2, 6703 HD Wageningen. The Netherlands.
Título: | Further improving the enzymic assay for cyanogens in cassava products.
Ano de publicação: | 1994
Fonte/Imprenta: | Acta Horticulturae, n.375, p.97-104, 1994.
ISSN: | 0567-7572
Idioma: | Inglês
Conteúdo: | A commonly used assay for routine determination of cyanogens in the enzymic assay developed by Cooke (1979), and improved by O'Brien et al. (1991). It compared well with earlier methods and was further improvedby more acceptable coloration and more accurate calculation of the cyanogenic glucosides and cyanohydrins (Essers et al., 1993). Isonicotinate/1,3-dimethyl barbiturate, the reagent used in the Konig reaction, was tested and found to have the following advantages over pyridine/pyrazolone, the color reagent used by Cooke and O'Brien et al.: it is less toxic and does not release repulsive vapors; it is quicker, cheaper and easier to handle; it has increased sensitivity and can be stored for longer. The new coloration has been applied successfully in laboratories in Uganda, The Netherlands and Malawi. An assay protocol is summarised. At Wageningen Agricultural University, the new assay was automated using a Gilson automated pipette station. The need for further improvements is discussed.
Palavras-Chave: | Analysis; Analysis reaction; Cyanide; Isonicotinate; Konig reaction; Linamarin.
Thesaurus Nal: | cassava.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)

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CNPMF - (3243-1) - ADC - AP - -- - 635.05 -  - 635.05 - DIS
CNPMF - (3527-1) - ADC - AP - -- - 635.05 -  - 635.05 - DIS