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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
06/07/2017 |
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Data da última atualização: |
21/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PIZAURO, L. J. L.; ALMEIDA, C. C. de; SOLTES, G. A.; SLAVIC, D.; ROSSI JUNIOR, O. D.; AVILA, F. A. de; ZAFALON, L. F.; MACINNES, J. I. |
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Afiliação: |
Lucas José Luduverio Pizauro, UNESP; Camila Chioda de Almeida, UNESP; Glenn A. Soltes, University of Guelph; Durda Slavic, University of Guelph; Oswaldo Durival Rossi Junior, UNESP; Fernando Antonio de Avila, UNESP; LUIZ FRANCISCO ZAFALON, CPPSE; Janet I. MacInnes, University of Guelph. |
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Título: |
Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization-time of flight mass spectrometry and cydB real-time quantitative PCR. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Veterinary Microbiology, v. 204, p. 8-14, 2017. |
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ISSN: |
0378-1135 |
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DOI: |
https://doi.org/10.1016/j.vetmic.2017.03.036 |
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Idioma: |
Inglês |
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Conteúdo: |
Incorrect identification of Staphylococcus spp. can have serious clinical and zoonotic repercussions. Accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization?time of flight mass spectrometry (MALDI-TOF MS) and/or cydB real- time quantitative PCR (qPCR) could be used to accurately identify coagulase negative Staphylococcus spp. (CoNS) obtained from buffalo milk and milking environment samples. Seventy-five of 84 CoNS isolates could be identified to the species level (score value >1.99) using MALDI-TOF MS. However, as determined by cytochrome d ubiquinol oxidase subunit II (cydB) qPCR and by 16S RNA and cydB gene sequencing, 10 S. agnetis strains were wrongly identified as S. hyicus by MALDI-TOF MS. In addition, 9 isolates identified by MALDI-TOF only to the genus level (score values between 1.70 and 1.99) could be identified to species by cydB qPCR. Our findings suggest that MALDI-TOF MS is a reliable method for rapid identification of S. chromogenes and S. epidermidis (species of interest both in human and veterinary medicine) and may be able to correctly identify other Staphylococcus spp. However, at present not all Staphylococcus spp. found in buffalo milk can be accurately identified by MALDI-TOF MS and for these organisms, the cydB qPCR developed in the current study may provide a reliable alternative method for rapid identification of CoNS species. |
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Palavras-Chave: |
CydB qPCR; Staphylococcus agnetis. |
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Thesaurus Nal: |
milk; Staphylococcus hyicus. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02359naa a2200277 a 4500
001 2072032
005 2017-11-21
008 2017 bl uuuu u00u1 u #d
022 $a0378-1135
024 7 $ahttps://doi.org/10.1016/j.vetmic.2017.03.036$2DOI
100 1 $aPIZAURO, L. J. L.
245 $aSpecies level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization-time of flight mass spectrometry and cydB real-time quantitative PCR.$h[electronic resource]
260 $c2017
520 $aIncorrect identification of Staphylococcus spp. can have serious clinical and zoonotic repercussions. Accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization?time of flight mass spectrometry (MALDI-TOF MS) and/or cydB real- time quantitative PCR (qPCR) could be used to accurately identify coagulase negative Staphylococcus spp. (CoNS) obtained from buffalo milk and milking environment samples. Seventy-five of 84 CoNS isolates could be identified to the species level (score value >1.99) using MALDI-TOF MS. However, as determined by cytochrome d ubiquinol oxidase subunit II (cydB) qPCR and by 16S RNA and cydB gene sequencing, 10 S. agnetis strains were wrongly identified as S. hyicus by MALDI-TOF MS. In addition, 9 isolates identified by MALDI-TOF only to the genus level (score values between 1.70 and 1.99) could be identified to species by cydB qPCR. Our findings suggest that MALDI-TOF MS is a reliable method for rapid identification of S. chromogenes and S. epidermidis (species of interest both in human and veterinary medicine) and may be able to correctly identify other Staphylococcus spp. However, at present not all Staphylococcus spp. found in buffalo milk can be accurately identified by MALDI-TOF MS and for these organisms, the cydB qPCR developed in the current study may provide a reliable alternative method for rapid identification of CoNS species.
650 $amilk
650 $aStaphylococcus hyicus
653 $aCydB qPCR
653 $aStaphylococcus agnetis
700 1 $aALMEIDA, C. C. de
700 1 $aSOLTES, G. A.
700 1 $aSLAVIC, D.
700 1 $aROSSI JUNIOR, O. D.
700 1 $aAVILA, F. A. de
700 1 $aZAFALON, L. F.
700 1 $aMACINNES, J. I.
773 $tVeterinary Microbiology$gv. 204, p. 8-14, 2017.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
06/11/2013 |
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Data da última atualização: |
23/01/2014 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ARAÚJO, E. S.; GUIMARÃES, A. P.; RUMJANEK, N. G.; BODDEY, R. M.; URQUIAGA, S. |
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Afiliação: |
Ediana Silva Araújo, bolsista de mestrado da UFRRJ; Ana Paula Guimarães, pós doutorando da UFRRJ; NORMA GOUVEA RUMJANEK, CNPAB; ROBERT MICHAEL BODDEY, CNPAB; SEGUNDO SACRAMENTO U CABALLERO, CNPAB. |
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Título: |
Efeito da soja inoculada com quatro estirpes de Bradyrhizobium spp quanto à fixação biológica de nitrogênio |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 13., 2013, Seropédica Ciência, saúde e esporte: caderno de resumos... Seropédica: Embrapa Agrobiologia, 2013. |
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Idioma: |
Português |
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Palavras-Chave: |
Bradyrzobium; Glycine max L. |
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Categoria do assunto: |
-- |
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URL: |
https://ojs.cnpab.embrapa.br/index.php/scjd/article/view/1703/416
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Marc: |
LEADER 00662nam a2200169 a 4500
001 1970540
005 2014-01-23
008 2013 bl uuuu u01u1 u #d
100 1 $aARAÚJO, E. S.
245 $aEfeito da soja inoculada com quatro estirpes de Bradyrhizobium spp quanto à fixação biológica de nitrogênio$h[electronic resource]
260 $aIn: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 13., 2013, Seropédica Ciência, saúde e esporte: caderno de resumos... Seropédica: Embrapa Agrobiologia$c2013
653 $aBradyrzobium
653 $aGlycine max L
700 1 $aGUIMARÃES, A. P.
700 1 $aRUMJANEK, N. G.
700 1 $aBODDEY, R. M.
700 1 $aURQUIAGA, S.
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