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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
13/01/2009 |
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Data da última atualização: |
14/01/2009 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MACHADO, M. A.; ÁSTUA, J. F.; AMARAL, A. M. do; MALOSSO, A.; MAZZAFERA, P. |
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Afiliação: |
M. A. Machado, APTA; Juliana Freitas-Ástua, CNPMF/APTA; A. M. do Amaral, APTA; A. Malosso, APTA; P. Mazzafera, APTA. |
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Título: |
Gene expression of carotenoid biosynthesis pathway and carotenoid content in different varieties of sweet orange (Citrus sinensis L. Osb.). |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan,China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 60. 105. |
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Idioma: |
Inglês |
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Conteúdo: |
Varieties of sweet orange are usually distinguishable by the content and quality of the juice. Such traits are important to characterize early, middle and late varieties. Juice quality is a complex trait that changes according with the content and quality of carotenoids, flavanoids, sugars and organic acids. The balance among such compounds is the main characteristic typifies the fruits of almost all varieties of sweet orange. The studies of biochemical pathway of carotenoid synthesis are hindered by the difficulties to evaluate enzyme activities which substracts and products are hydrophobic compounds. Thus the goal of this work was to evaluate the expression of genes of carotenoid synthesis in fruits of sweet orange Hamlin (early), Pera (middle), and Valencia (late), and to correlate with the content of carotenoids during fruit development. A pool of three fruits of 12 years old trees of sweet orange grafted on Rangpur lime was evaluate for fruit quality (juice content, Brix, ratio, content of total carotenoids), gene expression by Rt-qPCR in flavedo (epicarp) (Phytoene synthase, psy; Phytoene desaturase, pds; Z-carotene desaturase, zds; Carotenoid isomerase, crtiso; Lycopene cyclase, lcyb; Beta-hydroxylase, hyb; Zexanthin epoxidase, zep; Lycopene cyclase, lcye). Primers design was based on CitEST and public database. Rt-qPCR was performed using Sybergreen. The higher gene expression (7 to 19 times) was in sweet orange Hamlin at the end of the season (June, in Brazil), pointed out an active synthesis varieties the higher expression was in the middle of the cycle (July) before the picking time. Not always a direct relationship between gene expression and content of carotenoids could be inferred. MenosVarieties of sweet orange are usually distinguishable by the content and quality of the juice. Such traits are important to characterize early, middle and late varieties. Juice quality is a complex trait that changes according with the content and quality of carotenoids, flavanoids, sugars and organic acids. The balance among such compounds is the main characteristic typifies the fruits of almost all varieties of sweet orange. The studies of biochemical pathway of carotenoid synthesis are hindered by the difficulties to evaluate enzyme activities which substracts and products are hydrophobic compounds. Thus the goal of this work was to evaluate the expression of genes of carotenoid synthesis in fruits of sweet orange Hamlin (early), Pera (middle), and Valencia (late), and to correlate with the content of carotenoids during fruit development. A pool of three fruits of 12 years old trees of sweet orange grafted on Rangpur lime was evaluate for fruit quality (juice content, Brix, ratio, content of total carotenoids), gene expression by Rt-qPCR in flavedo (epicarp) (Phytoene synthase, psy; Phytoene desaturase, pds; Z-carotene desaturase, zds; Carotenoid isomerase, crtiso; Lycopene cyclase, lcyb; Beta-hydroxylase, hyb; Zexanthin epoxidase, zep; Lycopene cyclase, lcye). Primers design was based on CitEST and public database. Rt-qPCR was performed using Sybergreen. The higher gene expression (7 to 19 times) was in sweet orange Hamlin at the end of the season (June, in Brazil), poi... Mostrar Tudo |
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Palavras-Chave: |
Citros. |
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Thesagro: |
Variedade; Vitamina. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02435naa a2200205 a 4500
001 1655661
005 2009-01-14
008 2008 bl uuuu u00u1 u #d
100 1 $aMACHADO, M. A.
245 $aGene expression of carotenoid biosynthesis pathway and carotenoid content in different varieties of sweet orange (Citrus sinensis L. Osb.).
260 $c2008
520 $aVarieties of sweet orange are usually distinguishable by the content and quality of the juice. Such traits are important to characterize early, middle and late varieties. Juice quality is a complex trait that changes according with the content and quality of carotenoids, flavanoids, sugars and organic acids. The balance among such compounds is the main characteristic typifies the fruits of almost all varieties of sweet orange. The studies of biochemical pathway of carotenoid synthesis are hindered by the difficulties to evaluate enzyme activities which substracts and products are hydrophobic compounds. Thus the goal of this work was to evaluate the expression of genes of carotenoid synthesis in fruits of sweet orange Hamlin (early), Pera (middle), and Valencia (late), and to correlate with the content of carotenoids during fruit development. A pool of three fruits of 12 years old trees of sweet orange grafted on Rangpur lime was evaluate for fruit quality (juice content, Brix, ratio, content of total carotenoids), gene expression by Rt-qPCR in flavedo (epicarp) (Phytoene synthase, psy; Phytoene desaturase, pds; Z-carotene desaturase, zds; Carotenoid isomerase, crtiso; Lycopene cyclase, lcyb; Beta-hydroxylase, hyb; Zexanthin epoxidase, zep; Lycopene cyclase, lcye). Primers design was based on CitEST and public database. Rt-qPCR was performed using Sybergreen. The higher gene expression (7 to 19 times) was in sweet orange Hamlin at the end of the season (June, in Brazil), pointed out an active synthesis varieties the higher expression was in the middle of the cycle (July) before the picking time. Not always a direct relationship between gene expression and content of carotenoids could be inferred.
650 $aVariedade
650 $aVitamina
653 $aCitros
700 1 $aÁSTUA, J. F.
700 1 $aAMARAL, A. M. do
700 1 $aMALOSSO, A.
700 1 $aMAZZAFERA, P.
773 $tIn: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan,China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 60. 105.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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