|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
22/09/2006 |
|
Data da última atualização: |
22/09/2006 |
|
Autoria: |
RAMÍREZ CASILLAS, I. C.; SANTILLÁN FLORES, M. A.; ARELLANO REYNOSO, B.; MORALES ÁLVAREZ, F.; TENORIO GUTIÉRREZ, V. R. |
|
Título: |
Detección de secuencias nucleotídicas de Mycobacterium bovis a partir de adn de moco nasal de caprinos inoculados experimentalmente. |
|
Ano de publicação: |
2006 |
|
Fonte/Imprenta: |
Veterinaria México, v. 37, n. 2, p. 191-196, abr./jun., 2006. |
|
Idioma: |
Português |
|
Conteúdo: |
Excretion of M. bovis through aerosols and their inhalation is considered the main route of transmission and infection in cattle. The traditional methods used for monitoring the elimination of the microorganism by the nasal route are the bacteriological culture and the radiometric method. Recently developed molecular biology methods have favored the fast and reliable diagnosis of mycobacteria in the field. In the present work, the M-PCR technique was used to determine the time at which M. bovis' DNA could be detected from the nasal mucus of 14 experimentally-infected goats. Samples were taken from the nasal cavity at different time intervals after the challenge, and bacteriological culture and DNA amplification by M-PCR were carried out. Sixty days after challenge, the M-PCR techniques detected six out of 14 animals as positive and at day 90, these same six animals plus four more were detected as positive. No sample was positive to the bacteriological culture. It is known that the bacteriological isolation of mycobacteria is difficult and that the reduced number of viable bacteria in each sample influences the result of the diagnosis. In this work, the advantage of using the M-PCR technique in order to obtain positive results in just a short time was demonstrated, which favors a quick and timely identification of the animals that represent a risk of infection in the herd and that could represent reservoirs of the disease. |
|
Palavras-Chave: |
Mycobactereium bovis. |
|
Thesagro: |
Caprino; Doença Animal; Micobactéria. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02155naa a2200217 a 4500
001 1532145
005 2006-09-22
008 2006 bl uuuu u00u1 u #d
100 1 $aRAMÍREZ CASILLAS, I. C.
245 $aDetección de secuencias nucleotídicas de Mycobacterium bovis a partir de adn de moco nasal de caprinos inoculados experimentalmente.
260 $c2006
520 $aExcretion of M. bovis through aerosols and their inhalation is considered the main route of transmission and infection in cattle. The traditional methods used for monitoring the elimination of the microorganism by the nasal route are the bacteriological culture and the radiometric method. Recently developed molecular biology methods have favored the fast and reliable diagnosis of mycobacteria in the field. In the present work, the M-PCR technique was used to determine the time at which M. bovis' DNA could be detected from the nasal mucus of 14 experimentally-infected goats. Samples were taken from the nasal cavity at different time intervals after the challenge, and bacteriological culture and DNA amplification by M-PCR were carried out. Sixty days after challenge, the M-PCR techniques detected six out of 14 animals as positive and at day 90, these same six animals plus four more were detected as positive. No sample was positive to the bacteriological culture. It is known that the bacteriological isolation of mycobacteria is difficult and that the reduced number of viable bacteria in each sample influences the result of the diagnosis. In this work, the advantage of using the M-PCR technique in order to obtain positive results in just a short time was demonstrated, which favors a quick and timely identification of the animals that represent a risk of infection in the herd and that could represent reservoirs of the disease.
650 $aCaprino
650 $aDoença Animal
650 $aMicobactéria
653 $aMycobactereium bovis
700 1 $aSANTILLÁN FLORES, M. A.
700 1 $aARELLANO REYNOSO, B.
700 1 $aMORALES ÁLVAREZ, F.
700 1 $aTENORIO GUTIÉRREZ, V. R.
773 $tVeterinaria México$gv. 37, n. 2, p. 191-196, abr./jun., 2006.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
